Analytical impact of Metschnikowia pulcherrima in the volatile profile of Verdejo white wines
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Most wine aroma compounds, including the varietal fraction, are produced or released during wine production and derived from microbial activity. Varietal aromas, typically defined as terpenes and thiols, have been described as derived from their non-volatile precursors, released during wine fermentation by different yeast hydrolytic enzymes. The perception of these minority aroma compounds depends on the chemical matrix of the wine, especially on the presence of majority aroma compounds, such as esters or higher alcohols. Strategies aiming to reduce the production of these masking flavors are on the spotlight of enology research as a way to better define varietal standard profiles for the global market. Using a natural white must from Verdejo variety (defined as a thiol grape variety), here we describe the analytical and sensorial impact of using, in sequential inoculations, a selected strain of Metschnikowia pulcherrima, in combination with two different Saccharomyces cerevisiae strains. An increase in the levels of the thiol 4-MSP (4-methyl-4-sulfanylpentan-2-one) over its sensory threshold, together with a decrease in higher alcohol production, was observed when M. pulcherrima was used. This has an important impact on these wines, making them fruitier and fresher, always preferred by the sensory panel.
KeywordsNon-Saccharomyces Metschnikowia pulcherrima Polyfunctional thiols Verdejo wine Varietal profile
Funding for the research in this paper was provided by Agrovin S.A, under the framework of the CDTI-financed project IDI-20160102 and by Pago de Carraovejas S.L. CDTI-financed project IDI-20160750 (Centre for Industrial Technological Development, Spanish Ministry of Economy, Industry and Competitiveness, Spain).
Compliance with ethical standards
Ten experienced wine tasters were involved in the study.
Conflict of interest
I. Belda is now working at Biome Makers Inc.; E. Navascués is employee of Agrovin S.A.; all other authors declare that they have no conflict of interest.
- Chen K, Escott C, Loira I, del Fresno JM, Morata A, Tesfaye W, Calderon F, Suárez-Lepe JA, Han S, Benito S (2018) Use of non-Saccharomyces yeasts and oenological tannin in red winemaking: influence on colour, aroma and sensorial properties of young wines. Food Microbiol 69:51–63CrossRefPubMedGoogle Scholar
- Clemente-Jiménez JF, Mingorance-Cazorla L, Martínez-Rodríguez S, Las Heras-Vázquez FJ, Rodríguez-Vico F (2004) Molecular characterization and oenological properties of wine yeasts isolated during spontaneous fermentation of six varieties of grape must. Food Microbiol 21:149–155CrossRefGoogle Scholar
- Dubourdieu D, Tominaga T, Masneuf I, des Gachons CP, Murat ML (2006) The role of yeasts in grape flavor development during fermentation: the example of sauvignon blanc. Am J Enol Vitic 57:81–88Google Scholar
- González-Royo E, Pascual O, Kontoudakis N, Esteruelas M, Esteve-Zarzoso B, Mas A, Canals JM, Zamora F (2015) Oenological consequences of sequential inoculation with non-Saccharomyces yeasts (Torulaspora delbrueckii or Metschnikowia pulcherrima) and Saccharomyces cerevisiae in base wine for sparkling wine production. Eur Food Res Technol 240:999–1012CrossRefGoogle Scholar
- Kapaklis A (2014) Impact of specific volatile thiols on varietal aroma of wines produced from Greek and some international grape varieties. Ph.D. thesis, Justus- Liebig-University Gießen, Germany, URN: urn: nbn: de: hebis: 26- opus-122144. http://geb.uni-giessen.de/geb/volltexte/2016/12214/
- Lambrechts MG, Pretorius IS (2000) Yeast and its importance to wine aroma. A review. S Afr J Enol Vitic 21:97–129Google Scholar
- Rapp A, Yavas I, Hastrich U (1994) Einfache und schnelle Anreicherung (Kaltronmethode) von Aromastoffen des Weines und deren quantitative Bestimmung mittels Kapillargaschromatographie. Deutsche Lebensmittel-Rundschau 90:171–174Google Scholar
- Renault P, Coulon J, Moine V, Thibon C, Bely M (2016) Enhanced 3-sulfanylhexan-1-ol production in sequential mixed fermentation with Torulaspora delbrueckii/Saccharomyces cerevisiae reveals a situation of synergistic interaction between two industrial strains. Front Microbiol 7:293CrossRefPubMedPubMedCentralGoogle Scholar
- Sadoudi M, Tourdot-Maréchal R, Rousseaux S, Steyer D, Gallardo-Chacón JJ, Ballester J, Vichi S, Guerin-Schneider R, Caixach J, Alexandre H (2012) Yeast-yeast interactions revealed by aromatic profile analysis of sauvignon blanc wine fermented by single or co-culture of non-Saccharomyces and Saccharomyces yeasts. Food Microbiol 32:243–253CrossRefPubMedGoogle Scholar
- Tominaga T, Masneuf I, Dubourdieu D (1995) A S-cysteine conjugate precursor of aroma of white sauvignon. J Int Sci Vigne Vin 29:227–232Google Scholar