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Applied Microbiology and Biotechnology

, Volume 102, Issue 19, pp 8493–8500 | Cite as

Imaging mass spectrometry-guided fast identification of antifungal secondary metabolites from Penicillium polonicum

  • Jing Bai
  • Peng Zhang
  • Guanhu Bao
  • Jin-Gang Gu
  • Lida Han
  • Li-Wen Zhang
  • Yuquan Xu
Applied microbial and cell physiology

Abstract

The discovery of antibiotics from microorganisms using classic bioactivity screens suffers from heavy labor and high re-discovery rate. Recently, largely uncovered biosynthetic potentials were unveiled by new approaches, such as genetic manipulation of “silent” biosynthetic gene clusters, innovative data acquisition, and processing methods. In this work, a fast and efficient antibiotic identification pipeline based on the MALDI-TOF imaging mass spectrometry was applied to study the antifungal metabolites during the confrontation of two fungal species, Penicillium polonicum and wilt-inducing fungus Fusarium oxysporum. By visualizing the spatial distribution of metabolites directly on the microbial colony and surrounding media, we predicted the antifungal candidates before isolating pure compounds and individually testing their bioactivity, which subsequently guided the identification of target molecules using classic chromatographic methods. Via this procedure, we successfully identified two antifungal metabolites, fructigenine A and B, which belong to indole alkaloid class and were not reported for antifungal activity. Our work assigned new bioactivity to previously reported compounds and more importantly showed the efficiency of this approach towards quick discovery of bioactive compounds, which can help study the vast unexploited synthetic potential of microbial secondary metabolites.

Keywords

MALDI-TOF imaging mass spectrometry Penicillium polonicum Antifungal secondary metabolite Indole alkaloid 

Notes

Acknowledgments

We thank Agricultural Culture Collection of China (ACCC) and Professor Bingyan Xie from Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, for generously providing the Penicillium polonicum strains. We also thank the Research Facility Center of the Biotechnology Institute for providing equipment, HPLC-HRMS (Agilent, USA), and the software.

Funding

This work was supported by the “948” Project of the Ministry of Agriculture of China (2016-X43 to Y.X.), National Natural Science Foundation of China (31500079 to LW.Z. and 31570093 to Y.X.), The Agricultural Science and Technology Innovation Program (CAAS-XTCX2016012 to Y.X.), and the key project of the China National Tobacco Corporation (110201502019 to Y.X.).

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare no conflict of interest, and that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary material

253_2018_9218_MOESM1_ESM.pdf (1.2 mb)
ESM 1 (PDF 1252 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jing Bai
    • 1
  • Peng Zhang
    • 1
    • 2
  • Guanhu Bao
    • 2
  • Jin-Gang Gu
    • 3
  • Lida Han
    • 1
  • Li-Wen Zhang
    • 1
  • Yuquan Xu
    • 1
  1. 1.Biotechnology Research InstituteChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  2. 2.Natural Products Laboratory, International Joint Lab of Tea Chemistry and Health effects, State Key Laboratory of Tea Plant Biology and UtilizationAnhui Agricultural UniversityHefeiPeople’s Republic of China
  3. 3.Agricultural Culture Collection of China, Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China

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