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Applied Microbiology and Biotechnology

, Volume 102, Issue 15, pp 6647–6658 | Cite as

Cold plasma treatment triggers antioxidative defense system and induces changes in hyphal surface and subcellular structures of Aspergillus flavus

  • Juliana Šimončicová
  • Barbora Kaliňáková
  • Dušan Kováčik
  • Veronika Medvecká
  • Boris Lakatoš
  • Svetlana Kryštofová
  • Lucia Hoppanová
  • Veronika Palušková
  • Daniela Hudecová
  • Pavol Ďurina
  • Anna Zahoranová
Applied microbial and cell physiology
  • 116 Downloads

Abstract

The cold atmospheric-pressure plasma (CAPP) has become one of the recent effective decontamination technologies, but CAPP interactions with biological material remain the subject of many studies. The CAPP generates numerous types of particles and radiations that synergistically affect cells and tissues differently depending on their structure. In this study, we investigated the effect of CAPP generated by diffuse coplanar surface barrier discharge on hyphae of Aspergillus flavus. Hyphae underwent massive structural changes after plasma treatment. Scanning electron microscopy showed drying hyphae that were forming creases on the hyphal surface. ATR-FTIR analysis demonstrated an increase of signal intensity for C=O and C-O stretching vibrations indicating chemical changes in molecular structures located on hyphal surface. The increase in membrane permeability was detected by the fluorescent dye, propidium iodide. Biomass dry weight determination and increase in permeability indicated leakage of cell content and subsequent death. Disintegration of nuclei and DNA degradation confirmed cell death after plasma treatment. Damage of plasma membrane was related to lipoperoxidation that was determined by higher levels of thiobarbituric acid reactive species after plasma treatment. The CAPP treatment led to rise of intracellular ROS levels detected by fluorescent microscopy using 2′,7′-dichlorodihydrofluorescein diacetate. At the same time, antioxidant enzyme activities increased, and level of reduced glutathione decreased. The results in this study indicated that the CAPP treatment in A. flavus targeted both cell surface structures, cell wall, and plasma membrane, inflicting injury on hyphal cells which led to subsequent oxidative stress and finally cell death at higher CAPP doses.

Keywords

Antioxidant defense system Aspergillus flavus Cold atmospheric pressure plasma FTIR Lipid peroxidation Oxidative stress 

Notes

Funding information

This work was supported by the Slovak Research and Development Agency under the contract no. APVV-16-0216 and by a project for the building of infrastructure for the modern research of civilization diseases, ITMS 26230120006.

Compliance with ethical standard

Conflict of interest

The authors declare they have no conflict of interest.

Ethical approval

This paper does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Juliana Šimončicová
    • 1
  • Barbora Kaliňáková
    • 1
  • Dušan Kováčik
    • 2
  • Veronika Medvecká
    • 2
  • Boris Lakatoš
    • 1
  • Svetlana Kryštofová
    • 1
  • Lucia Hoppanová
    • 1
  • Veronika Palušková
    • 1
  • Daniela Hudecová
    • 1
  • Pavol Ďurina
    • 2
  • Anna Zahoranová
    • 2
  1. 1.Institute of Biochemistry and Microbiology, Faculty of Chemical and Food TechnologySlovak University of TechnologyBratislavaSlovakia
  2. 2.Department of Experimental Physics, Faculty of Mathematics, Physics, and InformaticsComenius UniversityBratislavaSlovakia

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