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The NT11, a novel fusion tag for enhancing protein expression in Escherichia coli

  • Thi Khoa My Nguyen
  • Mi Ran Ki
  • Ryeo Gang Son
  • Seung Pil PackEmail author
Biotechnologically relevant enzymes and proteins
  • 93 Downloads

Abstract

The Escherichia coli (E. coli) expression system has been widely used to produce recombinant proteins. However, in some heterologous expressions, there are still difficulties in large-scale production. The use of fusion partners is one of the strategies for improving the expression levels of proteins in E. coli host. Here, we demonstrate a novel fusion element, the NT11-tag, which enhances protein expression. The NT11-tag was derived from the first 11 amino acid residues within the N-terminal N-half domain of a duplicated carbonic anhydrase (dCA) from Dunaliella species. Previously, we have found that the tag improves expression of the C-half domain of dCA when linked to its N-terminus. To verify its use as a protein production enhancer tag, two kinds of CAs derived from Hahella chejuensis (Hc-CA) and Thermovibrio ammonifican (Ta-CA) and the yellow fluorescent protein (YFP) were used as model proteins to measure their increased expression upon fusion with the NT11-tag. The NT11-tag amplified protein expression in E. coli by 6.9- and 7.6-fold for Ta-CA and YFP, respectively. Moreover, the tag also enhanced the soluble expression of Hc-CA, Ta-CA, and YFP by 1.7-, 5.0-, and 3.2-fold, respectively. Furthermore, protein yield was increased without inhibiting protein function. These results indicate that the use of the NT11-tag is a promising method for improving protein production in E. coli.

Keywords

Fusion partner Solubility enhancement tag Esterase activity CO2 hydration 

Notes

Funding information

This work was supported by the Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) funded by the Ministry of Science, ICT and Future Planning, Korea (NRF-2015M1A5A1037054) and a Marine Biomaterials Research Center grant from the Marine Biotechnology Program funded by the Ministry of Oceans and Fisheries, Korea. This work was also supported by Research Fellow Funding grant funded by the Ministry of Education, Korea (NRF-2014R1A1A2008088) and BK21 plus.

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

253_2018_9595_MOESM1_ESM.pdf (643 kb)
ESM 1 (PDF 643 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Thi Khoa My Nguyen
    • 1
  • Mi Ran Ki
    • 1
  • Ryeo Gang Son
    • 1
  • Seung Pil Pack
    • 1
    Email author
  1. 1.Department of Biotechnology and BioinformaticsKorea UniversitySejongSouth Korea

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