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Quantitative determination of trace metals in single yeast cells by time-resolved ICP-MS using dissolved standards for calibration

  • Zihui Liu
  • Aifang Xue
  • Hao Chen
  • Shengqing LiEmail author
Methods and protocols

Abstract

Time-resolved inductively coupled plasma mass spectrometry (ICP-MS) for studying cellular heterogeneity or detecting metals in single cells draws increasing attention in the recent years. Considering the full width of a single-cell event is about 0.5–0.9 ms, dependent on the duration of the ion plume generation in typical ICP condition, dwell time shorter than the transient event was suggested for fully profiling it. Herein we investigated the effect of dwell time (0.1–10.0 ms) on the signal profiles of single-cell events, signal-to-background ratio, duty cycle of detection, the limit of detection, and the ability of cell counting by the time-resolved ICP-MS, using yeast (Saccharomyces cerevisiae) cells as example. Two calibration equations with respect to the length of dwell time (0.1 ms or 5.0 ms), simply using dissolved metal standard solutions, were constructed and successfully applied to the determination of K, Mg, Zn, Mn, and Cu in single yeast cells. The limit of detection (LOD, fg per cell) obtained at dwell time 0.1 ms was 1.68 (K), 0.29 (Mg), 0.17 (Zn), 0.01 (Mn), and 0.02 (Cu) for single-cell analysis of yeast cells; the LOD (fg per cell) at 5.0 ms was 80.0 (K), 10.3 (Mg), 1.6 (Zn), 0. 14 (Mn), and 0.24 (Cu), respectively. The results showed that a short dwell time leading to high signal-to-background ratio and low LOD was a prerequisite for the quantitative analysis of ultra-trace content of metals in single cells.

Keywords

Single cell analysis Time-resolved ICP-MS Dwell time Yeast (S. cerevisiaeTrace element 

Notes

Funding information

The authors gratefully appreciate the Fundamental Research Funds for the Central Universities (2662018JC009, 2662015PY047) and the financial support from the National Natural Science Foundation of China (21207045).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

253_2018_9587_MOESM1_ESM.pdf (920 kb)
ESM 1 (PDF 919 kb)

References

  1. Bandura DR, Baranov VI, Ornatsky OI, Antonov A, Kinach R, Lou X, Pavlov S, Vorobiev S, Dick JE, Tanner SD (2009) Mass cytometry: technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry. Anal Chem 81:6813–6822CrossRefGoogle Scholar
  2. Degueldre C, Favarger PY, Wold S (2006) Gold colloid analysis by inductively coupled plasma-mass spectrometry in a single particle mode. Anal Chim Acta 555:263–268CrossRefGoogle Scholar
  3. Groombridge AS, Miyashita S, Fujii S, Nagasawa K, Okahashi T, Ohata M, Umemura T, Takatsu A, Inagaki K, Chiba K (2013) High sensitive elemental analysis of single yeast cells (Saccharomyces cerevisiae) by time-resolved inductively-coupled plasma massspectrometry using a high efficiency cell introduction system. Anal Sci 29:597–603CrossRefGoogle Scholar
  4. Haraguchi H (2004) Metallomics as integrated biometal science. J Anal At Spectrom 19:5–14CrossRefGoogle Scholar
  5. Hineman A, Stephan C (2014) Effect of dwell time on single particle inductively coupled plasma mass spectrometry data acquisition quality. J Anal At Spectrom 29:1252–1257CrossRefGoogle Scholar
  6. Ho KS, Chan WT (2010) Time-resolved ICP-MS measurement for single-cell analysis and on-line cytometry. J Anal At Spectrom 25:1114–1122CrossRefGoogle Scholar
  7. Lau WY, Chun KH, Chan WT (2017) Correlation of single-cell ICP-MS intensity distributions for the study of heterogeneous cellular responses to environmental stresses. J Anal At Spectrom 32:807–815CrossRefGoogle Scholar
  8. Li FM, Armstrong DW, Houk RS (2005) Behavior of bacteria in the inductively coupled plasma: atomization and production of atomic ions for mass spectrometry. Anal Chem 77:1407–1413CrossRefGoogle Scholar
  9. Miyashita S, Groombridge AS, Fujii S, Minoda A, Takatsu A, Hioki A, Chiba K, Inagaki K (2014) Highly efficient single-cell analysis of microbial cells by time-resolved inductively coupled plasma mass spectrometry. J Anal At Spectrom 29:1598–1606CrossRefGoogle Scholar
  10. Montano MD, Majestic BJ, Jamting AK, Westerhoff P, Ranville JF (2016) Methods for the detection and characterization of silica colloids by microsecond spICP-MS. Anal Chem 88:4733–4741CrossRefGoogle Scholar
  11. Olesik JW, Gray PJ (2012) Considerations for measurement of individual nanoparticles or microparticles by ICP-MS: determination of the number of particles and the analyte mass in each particle. J Anal At Spectrom 27:1143–1155CrossRefGoogle Scholar
  12. Pace HE, Rogers NJ, Jarolimek CM, Coleman VA, Higgins CP, Ranville JF (2011) Determining transport efficiency for the purpose of counting and sizing nanoparticles via single particle inductively coupled plasma mass spectrometry. Anal Chem 83:9361–9369CrossRefGoogle Scholar
  13. Ramkorun-Schmidt B, Pergantis SA, Esteban-Fernandez D, Jakubowski N, Gunther D (2015) Investigation of a combined microdroplet generator and pneumatic nebulization system for quantitative determination of metal-containing nanoparticles using ICPMS. Anal Chem 87:8687–8694CrossRefGoogle Scholar
  14. Rodriguez MC, Garcia RAF, Blanco E, Bettmer J, Montes-Bayon M (2017) Quantitative evaluation of cisplatin uptake in sensitive and resistant individual cells by single-cell ICP-MS (SC-ICP-MS). Anal Chem 89:11491–11497CrossRefGoogle Scholar
  15. Shigeta K, Koellensperger G, Rampler E, Traub H, Rottmann L, Panne U, Okino A, Jakubowski N (2013) Sample introduction of single selenized yeast cells (Saccharomyces cerevisiae) by micro droplet generation into an ICP-sector field mass spectrometer for label-free detection of trace elements. J Anal At Spectrom 28:637–645CrossRefGoogle Scholar
  16. Tsang CN, Ho KS, Sun H, Chan WT (2011) Tracking bismuth antiulcer drug uptake in single Helicobacter pylori cells. J Am Chem Soc 133:7355–7357CrossRefGoogle Scholar
  17. Wang H, Wang B, Wang M, Zheng L, Chen H, Chai Z, Zhao Y, Feng W (2015) Time-resolved ICP-MS analysis of mineral element contents and distribution patterns in single cells. Analyst 140:523–531CrossRefGoogle Scholar
  18. Wang H, Wang M, Wang B, Zheng L, Chen H, Chai Z, Feng W (2017a) Interrogating the variation of element masses and distribution patterns in single cells using ICP-MS with a high efficiency cell introduction system. Anal Bioanal Chem 409:1415–1423CrossRefGoogle Scholar
  19. Wang H, Chen B, He M, Hu B (2017b) A facile droplet-chip-time-resolved inductively coupled plasma mass spectrometry online system for determination of zinc in single cell. Anal Chem 89:4931–4938CrossRefGoogle Scholar
  20. Wei X, Hu LL, Chen ML, Yang T, Wang JH (2016) Analysis of the distribution pattern of chromium species in single cells. Anal Chem 88:12437–12444CrossRefGoogle Scholar
  21. Yang B, Zhang Y, Chen B, He M, Yin X, Wang H, Li X, Hu B (2017) A multifunctional probe for ICP-MS determination and multimodal imaging of cancer cells. Biosens Bioelectron 96:77–83CrossRefGoogle Scholar
  22. Zhang L, Vertes A (2018) Single-cell mass spectrometry approaches to explore cellular heterogeneity. Angew Chem Int Ed 57:4466–4477CrossRefGoogle Scholar
  23. Zheng LN, Wang M, Wang B, Chen HQ, Ouyang H, Zhao YL, Chai ZF, Feng WY (2013) Determination of quantum dots in single cells by inductively coupled plasma mass spectrometry. Talanta 116:782–787CrossRefGoogle Scholar
  24. Zheng LN, Wang M, Zhao LC, Sun BY, Wang B, Chen HQ, Zhao YL, Chai ZF, Feng WY (2015) Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry. Anal Bioanal Chem 407:2383–2391CrossRefGoogle Scholar
  25. Zhou Y, Li H, Sun H (2017) Cytotoxicity of arsenic trioxide in single leukemia cells by time-resolved ICP-MS together with lanthanide tags. Chem Commun 53:2970–2973CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Agricultural Microbiology, College of ScienceHuazhong Agricultural UniversityWuhanChina

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