Abstract
In the post-genome age, proteomics is receiving significant attention because they provide an invaluable source of biological structures and functions at the protein level. The search for disease-specific biomarkers for diagnostic and/or therapeutic applications is one of the areas that proteomics is having a significant impact. Thus, the identification of a “good” biomarker enables a more accurate early diagnosis and prognosis of disease. Rapid advancements in mass spectrometry (MS) instrumentation, liquid chromatography MS (LCMS), protein microarray technology, and other protein profiling methodologies have a substantial expansion of our toolbox to identify disease-specific protein and peptide biomarkers. This review covers a selection of widely used proteomic technologies for biomarker discovery. In addition, we describe the most commonly used approaches for diagnosis based on proteomic biomarkers and further discuss trends and critical challenges during development of cost-effective rapid diagnostic tests and microfluidic diagnostic systems based on proteomic biomarkers.
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Abdallah C, Dumas-Gaudot E, Renaut J, Sergeant K (2012) Gel-based and gel-free quantitative proteomics approaches at a glance. International Journal of Plant Genomics 20:1–17
Aebersold R (2003) A mass spectrometric journey into protein and proteome research. J Am Soc Mass Spectrom 14(7):685–695
Aebersold R, Mann M (2003) Mass spectrometry-based proteomics. Nature 422(6928):198–207. doi:10.1038/nature01511
Alban A, David SO, Bjorkesten L, Andersson C, Sloge E, Lewis S, Currie I (2003) A novel experimental design for comparative two-dimensional gel analysis: two-dimensional difference gel electrophoresis incorporating a pooled internal standard. Proteomics 3(1):36–44. doi:10.1002/pmic.200390006
Allred DC, Harvey JM, Berardo M, Clark GM (1998) Prognostic and predictive factors in breast cancer by immunohistochemical analysis. Mod Pathol 11(2):155–168
Andersen LP, Espersen F (1992) Immunoglobulin G antibodies to Helicobacter pylori in patients with dyspeptic symptoms investigated by the western immunoblot technique. J Clin Microbiol 30(7):1743–1751
Beretov J, Wasinger VC, Graham PH, Millar EK, Kearsley JH, Li Y (2014) Proteomics for breast cancer urine biomarkers. Adv Clin Chem 63:123–167
Bertrand E, Faupel M (2007) Subcellular proteomics: from cell deconstruction to system reconstruction. Springer, Dordrecht, London
Bhalla S, Tandon S, Satyamoorthy K (2010) Salivary proteins and early childhood caries: a gel electrophoretic analysis. Contemp Clin Dent 1(1):17–22. doi:10.4103/0976-237X.62515
Chandramouli K, Qian PY (2009) Proteomics: challenges, techniques and possibilities to overcome biological sample complexity. Hum Genomics Proteomics 2009 doi:10.4061/2009/239204
Chen Y, Azman SN, Kerishnan JP, Zain RB, Chen YN, Wong Y-L, Gopinath SC (2014) Identification of host-immune response protein candidates in the sera of human oral squamous cell carcinoma patients
Chen Y, Chan CK, Kerishnan JP, Lau YL, Wong Y-L, Gopinath SC (2015) Identification of circulating biomarkers in sera of Plasmodium knowlesi-infected malaria patients—comparison against Plasmodium vivax infection. BMC Infect Dis 15(1):49
Chen Z, Mauk MG, Wang J, Abrams WR, Corstjens PLAM, Niedbala RS, Malamud D, Bau HH (2007) A microfluidic system for saliva-based detection of infectious diseases. Ann N Y Acad Sci 1098:429–436
Chenau J, Michelland S, Sidibe J, Seve M (2008) Peptides OFFGEL electrophoresis: a suitable pre-analytical step for complex eukaryotic samples fractionation compatible with quantitative iTRAQ labeling. F Proteome Sci 6:6–9
Cleary MD, Meiering CD, Jan E, Guymon R, Boothroyd JC (2005) Biosynthetic labeling of RNA with uracil phosphoribosyltransferase allows cell-specific microarray analysis of mRNA synthesis and decay. Nat Biotechnol 23(2):232–237
Corp M (1996) A short guide: developing immunochromatographic test strips. Millipore Bedford, MA
Damhorst GL, Murtagh M, Rodriguez WR, Bashir R (2015) Microfluidics and nanotechnology for detection of global infectious diseases. Proc IEEE 103(2):150–160
Das S, Sylvain MR, Fernand VE, Losso JN, El-Zahab B, Warner IM (2011) Positive cooperative mechanistic binding of proteins at low concentrations: a comparison of poly (sodium N-undecanoyl sulfate) and sodium dodecyl sulfate. J Colloid Interface Sci 363(2):585–594
Diez P, Dasilva N, Gonzalez-Gonzalez M, Matarraz S, Casado-Vela J, Orfao A, Fuentes M (2012) Data analysis strategies for protein microarrays. Microarrays (Basel) 1(2):64–83. doi:10.3390/microarrays1020064
Egidi E, Sestili F, Janni M, D’Ovidio R, Lafiandra D, Ceriotti A, Vensel WH, Kasarda DD, Masci S (2014) An asparagine residue at the N-terminus affects the maturation process of low molecular weight glutenin subunits of wheat endosperm. BMC Plant Biol 14(1):64
El-Ali J, Sorger PK, Jensen KF (2006) Cells on chips. Nature 442(7101):403–411
el-Zaatari FA, Oweis SM, Graham DY (1997) Uses and cautions for use of polymerase chain reaction for detection of Helicobacter pylori. Dig Dis Sci 42(10):2116–2119
Elshimali YI, Khaddour H, Sarkissyan M, Wu Y, Vadgama JV (2013) The clinical utilization of circulating cell free DNA (CCFDNA) in blood of cancer patients. Int J Mol Sci 14(9):18925–18958
Eteshola E, Balberg M (2004) Microfluidic ELISA: on-chip flourescence imaging. Biomed Microdevices 6:7–9. doi:10.1023/B:BMMD.0000013360.65653.c2
Eteshola E, Leckband D (2001) Development and characterization of an ELISA assay in PDMS microfluidic channels. Sensors Actuators B Chem 72(2):129–133
Feng X, Wen H, Zhang Z, Chen X, Ma X, Zhang J, Qi X, Bradshaw H, Vuitton D, Craig PS (2010) Dot immunogold filtration assay (DIGFA) with multiple native antigens for rapid serodiagnosis of human cystic and alveolar echinococcosis. Acta Trop 113(2):114–120. doi:10.1016/j.actatropica.2009.10.003
Francis G, Stein S (2015) Circulating cell-free tumour DNA in the management of cancer. Int J Mol Sci 16(6):14122–14142. doi:10.3390/ijms160614122
Ge Y, Molloy MP, Chamberlain JS, Andrews PC (2003) Proteomic analysis of mdx skeletal muscle: great reduction of adenylate kinase 1 expression and enzymatic activity. Proteomics 3(10):1895–1903. doi:10.1002/pmic.200300561
Gevaert K, Vandekerckhove J (2000) Protein identification methods in proteomics. Electrophoresis 21(6):1145–1154. doi:10.1002/(SICI)1522-2683(20000401)21:6<1145::AID-ELPS1145>3.0
Gharbi S, Gaffney P, Yang A, Zvelebil MJ, Cramer R, Waterfield MD, Timms JF (2002) Evaluation of two-dimensional differential gel electrophoresis for proteomic expression analysis of a model breast cancer cell system. Mol Cell Proteomics 1(2):91–98
Glassman MS, Dallal S, Berezin SH, Bostwick HE, Newman LJ, Perez-Perez GI, Blaser MJ (1990) Helicobacter pylori-related gastroduodenal disease in children. Diagnostic utility of enzyme-linked immunosorbent assay. Dig Dis Sci 35(8):993–997
Gorg A, Weiss W, Dunn MJ (2004) Current two-dimensional electrophoresis technology for proteomics. Proteomics 4(12):3665–3685. doi:10.1002/pmic.200401031
Gygi SP, Aebersold R (1999) Absolute quantitation of 2-D protein spots. Methods Mol Biol 112:417–421
Hall DA, Ptacek J, Snyder M (2007) Protein microarray technology. Mech Ageing Dev 128(1):161–167
Hamdan MH (2006) Cancer biomarkers: analytical techniques for discovery. Wiley & Sons. Inc., Hoboken, New Jersey
Han X, Aslanian A, Yates JR 3rd (2008) Mass spectrometry for proteomics. Curr Opin Chem Biol 12(5):483–490
Heller M, Michel PE, Morier P, Crettaz D, Wenz C, Tissot JD, Reymond F, Rossier JS (2005) Two-stage off-gel (TM) isoelectric focusing: protein followed by peptide fractionation and application to proteome analysis of human plasma. Electrophoresis 26(6):1174–1188. doi:10.1002/elps.200410106
Hujakka H, Koistinen V, Kuronen I, Eerikainen P, Parviainen M, Lundkvist A, Vaheri A, Vapalahti O, Narvanen A (2003) Diagnostic rapid tests for acute hantavirus infections: specific tests for Hantaan, Dobrava and Puumala viruses versus a hantavirus combination test. J Virol Methods 108(1):117–122
ICN2 N (2015) Nanomicrofluidics. Publishing NANO Bioelectric & Biosensor Group. http://www.nanobiosensors.org/research/electrochemical-sensors-based-on-nanostructurated-materials-i-e-carbon-nanotubes-etc-for-environmental-monitoring-and-other-industrial-applications/. May 13 2015
Ikeda M, Yamaguchi N, Tani K, Nasu M (2006) Rapid and simple detection of food poisoning bacteria by bead assay with a microfluidic chip-based system. J Microbiol Methods 67:241–247. doi:10.1016/j.mimet.2006.03.014
Inagaki N, Katsuta K (2004) Large gel two-dimensional electrophoresis: improving recovery of cellular proteome. Current Proteomics 1:35–39
Jonscher KR, Yates JR 3rd (1997) The quadrupole ion trap mass spectrometer—a small solution to a big challenge. Anal Biochem 244(1):1–15
Kai J, Puntambekar A, Santiago N, Lee SH, Sehy DW, Moore V, Han J, Ahn CH (2012) A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA). Lab Chip 12(21):4257–4262. doi:10.1039/c2lc40585g
Karami A, Naghavi KH, Sorouri R, Ranjbar R, Khalilpour A (2008) Use of a MAMA-PCR method to detect GyrA mutations in nalidixic acid resistant clinical isolates of Escherichia coli. Iran J Public Health 37(1):42–47
Karami A, Biramijamal F, Ghanei M, Arjmand S, Eshraghi M, Khalilpoor A (2007) New p53 gene mutation in non-cancerous mustard gas exposed lung. Iranian Journal of Basic Medical Sciences 10(2):111–117
Karami A, Ahmadi Z, Safiri Z, Khalilpour A, Morovati S (2006a) Development of an ultra rapid and simple multiplex polymerase chain reaction technique for detection of Salmonella typhi. Saudi Med J 27(8):1134–1138
Karami A, Hindeersson P, Hoiby N, Morovvati S, Khalilpour A (2006b) Linear and circular plasmids in skin and cerebrospinal fluid isolates of Borrelia burgdorferi agent of Lyme disease. Pak J Biol Sci 6(15):2787–2793
Khalilpour A (2016) Helicobacter pylori biomarkers for diagnostic kits and vaccines. LAP LAMBERT Academic Publishing
Khalilpour A, Kazemzadeh-Narbat M, Tamayol A, Oklu R, Khademhosseini A (2016) Biomarkers and diagnostic tools for detection of Helicobacter pylori. Appl Microbiol Biotechnol 100(11):4723–4734. doi:10.1007/s00253-016-7495-7
Khalilpour A, Osman S, Yunus MH, Santhanam A, Vellasamy N, Noordin R (2014a) Helicobacter pylori recombinant UreG protein: cloning, expression, and assessment of its seroreactivity. BMC Res Notes 7:809. doi:10.1186/1756-0500-7-809
Khalilpour A, Sadjjadi SM, Moghadam ZK, Yunus MH, Zakaria ND, Osman S, Noordin R (2014b) Lateral flow test using Echinococcus granulosus native antigen B and comparison of IgG and IgG4 dipsticks for detection of human cystic echinococcosis. AmJTrop Med Hyg 91(5):994–999. doi:10.4269/ajtmh.14-0170
Khalilpour A, Santhanam A, Wei LC, Saadatnia G, Velusamy N, Osman S, Mohamad AM, Noordin R (2013) Antigenic proteins of Helicobacter pylori of potential diagnostic value. Asian Pac J Cancer Prev 14(3):1635–1642
Khalilpour A, Santhanam A, Wei LC, Mohamad AM, Osman S, Noordin R (2012) Helicobacter pylori proteins with diagnostic potential identified using proteomic approach. Int J Infect Dis 16(Supplement 1):e396–e397
Klose J, Kobalz U (1995) Two-dimensional electrophoresis of proteins: an updated protocol and implications for a functional analysis of the genome. Electrophoresis 16(6):1034–1059
Knowles MR, Cervino S, Skynner HA, Hunt SP, de Felipe C, Salim K, Meneses-Lorente G, McAllister G, Guest PC (2003) Multiplex proteomic analysis by two-dimensional differential in-gel electrophoresis. Proteomics 3(7):1162–1171. doi:10.1002/pmic.200300437
Krijgsveld J (2012) Proteiomics of biological systems: protein phosphorylation using mass spectrometry techniques. By Bryan M. Ham. Wiley Online Library
Kulasingam V, Diamandis EP (2008) Strategies for discovering novel cancer biomarkers through utilization of emerging technologies. Nat Clin Pract Oncol 5(10):588–599. doi:10.1038/ncponc1187
Lai S, Wang S, Luo J, Lee LJ, Yang ST, Madou MJ (2004) Design of a compact disk-like microfluidic platform for enzyme-linked immunosorbent assay. Anal Chem 76:1832–1837. doi:10.1021/ac0348322
Lee WG, Kim Y-G, Chung BG, Demirci U, Khademhosseini A (2010a) Nano/microfluidics for diagnosis of infectious diseases in developing countries. Adv Drug Deliv Rev 62:449–457. doi:10.1016/j.addr.2009.11.016
Lee WG, Kim YG, Chung BG, Demirci U, Khademhosseini A (2010b) Nano/microfluidics for diagnosis of infectious diseases in developing countries. Adv Drug Deliv Rev 62(4–5):449–457. doi:10.1016/j.addr.2009.11.016
Lee Y-F, Lien K-Y, Lei H-Y, Lee G-B (2009) An integrated microfluidic system for rapid diagnosis of dengue virus infection. Biosensors & Bioelectronics 25:745–752. doi:10.1016/j.bios.2009.08.020
Link AJ, Hays LG, Carmack EB, Yates JR 3rd (1997) Identifying the major proteome components of Haemophilus influenzae type-strain NCTC 8143. Electrophoresis 18(8):1314–1334. doi:10.1002/elps.1150180808
Lion N, Rohner TC, Dayon L, Arnaud IL, Damoc E, Youhnovski N, Wu ZY, Roussel C, Josserand J, Jensen H, Rossier JS, Przybylski M, Girault HH (2003) Microfluidic systems in proteomics. Electrophoresis 24(21):3533–3562. doi:10.1002/elps.200305629
Liu Y, Wang H, Huang J, Yang J, Liu B, Yang P (2009) Microchip-based ELISA strategy for the detection of low-level disease biomarker in serum. Anal Chim Acta 650:77–82. doi:10.1016/j.aca.2009.06.048
Lodish H, Berk A, Zipursky SL, Matsudaira P, Baltimore D, Darnell J (2000a) Purifying, detecting, and characterizing proteins
Lodish HF, Berk A, Zipursky SL, Matsudaira P, Baltimore D, Darnell J (2000b) Molecular cell biology, 4th edn. Citeseer, NY
Macfarlane DE (1989) Two dimensional benzyldimethyl-n-hexadecylammonium chloride-sodium dodecyl sulfate preparative polyacrylamide gel electrophoresis: a high capacity high resolution technique for the purification of proteins from complex mixtures. Anal Biochem 176(2):457–463
Madu CO, Lu Y (2010) Novel diagnostic biomarkers for prostate cancer. J Cancer 1:150
Maghsoudi N, Khalilpour A, Kamali M, Zeinoddini M (2007) Cloning and expression of coxsakievirus B3 viral protein-1 in E. coli. Iran Biomed J 11(3):147–152
Mairhofer J, Roppert K, Ertl P (2009) Microfluidic systems for pathogen sensing: a review. Sensors (Basel) 9(6):4804–4823. doi:10.3390/s90604804
Mamuti W, Yamasaki H, Sako Y, Nakaya K, Nakao M, Lightowlers MW, Ito A (2002) Usefulness of hydatid cyst fluid of Echinococcus granulosus developed in mice with secondary infection for serodiagnosis of cystic Echinococcosis in humans. Clin Diagn Lab Immunol 9(3):573–576
Manes G, Zanetti MV, Piccirillo MM, Lombardi G, Balzano A, Pieramico O (2005) Accuracy of a new monoclonal stool antigen test in post-eradication assessment of Helicobacter pylori infection: comparison with the polyclonal stool antigen test and urea breath test. Dig Liver Dis 37(10):751–755
Marzese DM, Hirose H, Hoon DS (2013) Diagnostic and prognostic value of circulating tumor-related DNA in cancer patients. Expert Rev Mol Diagn 13(8):827–844. doi:10.1586/14737159.2013.845088
Matthiesen R (2007) Mass spectrometry data analysis in proteomics Humana press Totowa. Jersey, New
McDonald JC, Whitesides GM (2002) Poly(dimethylsiloxane) as a material for fabricating microfluidic devices. Acc Chem Res 35:491–499. doi:10.1021/ar010110q
Messina GA, Panini NV, Martinez NA, Raba J (2008) Microfluidic immunosensor design for the quantification of interleukin-6 in human serum samples. Anal Biochem 380:262–267. doi:10.1016/j.ab.2008.05.055
Michael Hamacher KM, Stühler K, Dipl.-Oec. André van Hall, Warscheid B, Meyer HE (2006) Proteomics in Drug Research
Michel PE, Reymond F, Arnaud IL, Josserand J, Girault HH, Rossier JS (2003) Protein fractionation in a multicompartment device using off-gel (TM) isoelectric focusing. Electrophoresis 24(1–2):3–11
Miwa H, Akamatsu S, Tachikawa T, Sogabe T, Ohtaka K, Nagahara A, Sugiyama Y, Sato N (2001) On-site diagnosis of H. pylori infection by urine. Diagn Microbiol Infect Dis 39(2):95–97
Moghadam ZK, Ghaffarifar F, Khalilpour A, Abdul Aziz F, Saadatnia G, Noordin R (2013) IgG4 detection of Echinococcus granulosus paramyosin is a useful diagnostic test for human hydatidosis. Clin Vaccine Immunol 20(4):501–505. doi:10.1128/CVI.00019-13
Molloy MP, Herbert BR, Slade MB, Rabilloud T, Nouwens AS, Williams KL, Gooley AA (2000) Proteomic analysis of the Escherichia coli outer membrane. Eur J Biochem 267(10):2871–2881
Molloy MP, Herbert BR, Walsh BJ, Tyler MI, Traini M, Sanchez JC, Hochstrasser DF, Williams KL, Gooley AA (1998) Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis 19(5):837–844. doi:10.1002/elps.1150190539
Moore C (2009) Introduction to western blotting. AbD serotec
Morand JP, Macri J, Adeli K (2005) Proteomic profiling of hepatic endoplasmic reticulum-associated proteins in an animal model of insulin resistance and metabolic dyslipidemia. J Biol Chem 280(18):17626–17633
Moreda-Pineiro A, Garcia-Otero N, Bermejo-Barrera P (2014) A review on preparative and semi-preparative offgel electrophoresis for multidimensional protein/peptide assessment. Anal Chim Acta 836:1–17. doi:10.1016/j.aca.2014.04.053
Ng AH, Uddayasankar U, Wheeler AR (2010) Immunoassays in microfluidic systems. Anal Bioanal Chem 397(3):991–1007. doi:10.1007/s00216-010-3678-8
Nooradin R, Santhanam A, Khalilpour A, Lee CW, Osman S (2013) Helicobacter pylori proteins for diagnostic kit and vaccine
Novo P, França Prazeres DM, Chu V, Conde JP (2011) Microspot-based ELISA in microfluidics: chemiluminescence and colorimetry detection using integrated thin-film hydrogenated amorphous silicon photodiodes. Lab Chip 11:4063
Olut AI, Erguven S, Emri S, Ozunlu H, Akay H (2005) Diagnostic value of a dot immunobinding assay for human pulmonary hydatidosis. Korean J Parasitol 43(1):15–18
Pan S, Aebersold R, Chen R, Rush J, Goodlett DR, McIntosh MW, Zhang J, Brentnall TA (2009) Mass spectrometry based targeted protein quantification: methods and applications. J Proteome Res 8(2):787–797. doi:10.1021/pr800538n
Pappas MG, Schantz PM, Cannon LT Sr, Wahlquist SP (1986) Dot-ELISA for the rapid serodiagnosis of human hydatid disease. Diagn Immunol 4(6):271–276
Parsa H, Chin CD, Mongkolwisetwara P, Lee BW, Wang JJ, Sia SK (2008) Effect of volume- and time-based constraints on capture of analytes in microfluidic heterogeneous immunoassays. Lab Chip 8(12):2062–2070. doi:10.1039/b813350f
Pelerito A, Oleastro M, Lopes AI, Ramalho P, Cabral J, Monteiro L (2006) Evaluation of rapid test assure helicobacter pylori for diagnosis of H. pylori in pediatric population. J Microbiol Methods 66(2):331–335
Rabilloud T, Lelong C (2011) Two-dimensional gel electrophoresis in proteomics: a tutorial. J Proteome 74(10):1829–1841. doi:10.1016/j.jprot.2011.05.040
Rabilloud T, Vaezzadeh AR, Potier N, Lelong C, Leize-Wagner E, Chevallet M (2009) Power and limitations of electrophoretic separations in proteomics strategies. Mass Spectrom Rev 28(5):816–843
Reisdorph NA, Reisdorph R, Bowler R, Broccardo C (2009) Proteomics methods and applications for the practicing clinician. Ann Allergy Asthma Immunol 102(6):523–529
Riazi M, Zainul FZ, Bahaman AR, Amran F, Khalilpour A (2014) Role of 72 kDa protein of Leptospira interrogans as a diagnostic marker in acute leptospirosis. Indian J Med Res 139(2):308–313
Ros A, Faupel M, Mees H, Oostrum J, Ferrigno R, Reymond F, Michel P, Rossier JS, Girault HH (2002) Protein purification by off-gel electrophoresis. Proteomics 2(2)
Saadatnia G, Ghaffarifar F, Khalilpour A, Amerizadeh A, Rahmah N (2011) A Toxoplasma gondii 10 kDa in vitro excretory secretory antigen reactive with human IgM and IgA antibodies. Trop Biomed 28(3):606–614
Saidin S, Yunus MH, Zakaria ND, Razak KA, Huat LB, Othman N, Noordin R (2014) Production of recombinant Entamoeba histolytica pyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess. BMC Infect Dis 14:182. doi:10.1186/1471-2334-14-182
Sasidharan S, Uyub AM (2009) Antibody response to Helicobacter pylori excretory antigen and the cross reaction study. J Immunoassay Immunochem 30(1):70–81
Seillier-Moiseiwitsch F, Trost DC, Moiseiwitsch J (2002) Statistical methods for proteomics. Methods Mol Biol 184:51–80
Shirran SL, Botting CH (2010) A comparison of the accuracy of iTRAQ quantification by nLC-ESI MSMS and nLC-MALDI MSMS methods. J Proteome 73(7):1391–1403
Simor AE, Lin E, Saibil F, Cohen L, Louie M, Pearen S, Donhoffer HA (1996) Evaluation of enzyme immunoassay for detection of salivary antibody to Helicobacter pylori. J Clin Microbiol 34(3):550–553
Spengler B (1997) Post-source decay analysis in matrix-assisted laser desorption/ionization mass spectrometry of biomolecules. J Mass Spectrom 32:1019–1036
Stokes D, Griffin GD, Vo-Dinh T (2001a) Detection of E. coli using a microfluidics-based antibody biochip detection system. Fresenius J Anal Chem 369(3–4):295–301
Stokes DL, Griffin GD, Vo-Dinh T (2001b) Detection of E. coli using a microfluidics-based antibody biochip detection system. Fresenius J Anal Chem 369:295–301. doi:10.1007/s002160000660
Tanca A, Palomba A, Deligios M, Cubeddu T, Fraumene C, Biosa G, Pagnozzi D, Addis MF, Uzzau S (2013) Evaluating the impact of different sequence databases on metaproteome analysis: insights from a lab-assembled microbial mixture. PLoS One 8(12):e82981
Tao SC, Chen CS, Zhu H (2007) Applications of protein microarray technology. Comb Chem High Throughput Screen 10(8):706–718
Taylor JA, Johnson RS (2001) Implementation and uses of automated de novo peptide sequencing by tandem mass spectrometry. Anal Chem 73:2594–2604
Tonge R, Shaw J, Middleton B, Rowlinson R, Rayner S, Young J, Pognan F, Hawkins E, Currie I, Davison M (2001) Validation and development of fluorescence two-dimensional differential gel electrophoresis proteomics technology. Proteomics 1(3):377–396. doi:10.1002/1615-9861(200103)1:3<377::AID-PROT377>3.0.CO;2-6
Unlü M, Morgan ME, Minden JS (1997) Difference gel electrophoresis: a single gel method for detecting changes in protein extracts. Electrophoresis 18:2071–2077
Van Hoof D, Heck AJ, Krijgsveld J, Mummery CL (2008) Proteomics and human embryonic stem cells. Stem Cell Res 1(3):169–182. doi:10.1016/j.scr.2008.05.003
Veenstra, D T, Smith RD (2003) Proteome characterization and proteomics
Viswanathan S, Unlu M, Minden JS (2006) Two-dimensional difference gel electrophoresis. Nat Protoc 1(3):1351–1358
Weiss LM, Kim K (2011) Toxoplasma gondii: the model apicomplexan. Perspectives and methods. Academic Press
Wessling B (1996) Wessling, conductive polymer/solvent systems: solutions or dispersions?, University of Wisconsin-Madison: making and conjugating colloidal metals
Xiang Q, Hu G, Gao Y, Li D (2006) Miniaturized immunoassay microfluidic system with electrokinetic control. Biosens Bioelectron 21(10):2006–2009
Yakovleva J, Davidsson R, Lobanova A, Bengtsson M, Eremin S, Laurell T, Emneus J (2002) Microfluidic enzyme immunoassay using silicon microchip with immobilized antibodies and chemiluminescence detection. Anal Chem 74(13):2994–3004
Yates J (2016) MudPIT (Multidimensional Protein Identification Technology). Publishing IMAT program. https://imat.cancer.gov/about/outputs/tech/mudpit.asp. 20 Nov 2016
Yates JR 3rd, Link AJ, Schieltz D (2000) Direct analysis of proteins in mixtures. Application to protein complexes. Methods Mol Biol 146:17–26. doi:10.1385/1-59259-045-4:17
Yates JR 3rd, McCormack AL, Link AJ, Schieltz D, Eng J, Hays L (1996) Future prospects for the analysis of complex biological systems using micro-column liquid chromatography-electrospray tandem mass spectrometry. Analyst 121(7):65R–76R
Zhao J, Fan Y-X, Yang Y, Liu D-L, Wu K, Wen F-B, Zhang C-Y, Zhu D-Y, Zhao S (2015) Identification of potential plasma biomarkers for esophageal squamous cell carcinoma by a proteomic method. International Journal of Clinical and Experimental Pathology 8(2):1535
Zhou J, Khodakov DA, Ellis AV, Voelcker NH (2012) Surface modification for PDMS-based microfluidic devices. Electrophoresis 33:89–104
Zuniga-Noriega JR, Bosques-Padilla FJ, Perez-Perez GI, Tijerina-Menchaca R, Flores-Gutierrez JP, Maldonado Garza HJ, Garza-Gonzalez E (2006) Diagnostic utility of invasive tests and serology for the diagnosis of Helicobacter pylori infection in different clinical presentations. Arch Med Res 37(1):123–128
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The authors would like to thank Prof. Ali Khademhosseini and Dr. Ali Tamayol from Harvard Medical School for their helpful comments and suggestions.
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Khalilpour, A., Kilic, T., Khalilpour, S. et al. Proteomic-based biomarker discovery for development of next generation diagnostics. Appl Microbiol Biotechnol 101, 475–491 (2017). https://doi.org/10.1007/s00253-016-8029-z
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DOI: https://doi.org/10.1007/s00253-016-8029-z