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Operational temperature regulates anodic biofilm growth and the development of electrogenic activity

Abstract

The operational temperature of microbial fuel cell reactors influences biofilm development, and this has an impact on anodic biocatalytic activity. In this study, we compared three microbial fuel cell (MFC) reactors acclimated at 10°C, 20°C and 35°C to investigate the effect on biomass development, methanogenesis and electrogenic activity over time. The start-up time was inversely influenced by temperature, but the amount of biomass accumulation increased with increased temperatures, the 10°C, 20°C and 35°C acclimated biofilms resulted in 0.57, 0.82 and 5.43 g biomass (volatile suspended solids) per litre respectively at 56 weeks of operation. Biofilm build-up on the 35°C anode was further demonstrated by scanning electron microscopy, which showed large aggregations of biomass accumulating on the anode when compared to 10°C and 20°C biofilms. Biomass accumulation had a direct impact on biocatalytic performance, with the maximum power at 35°C after 60 weeks of operation being 2.14 W m−3 and power densities for the 10°C and 20°C reactors being and 4.29 W m−3. Methanogenic activity was also shown to be higher at 35°C, with a rate of 10.1 mmol CH4 biofilm per gram of volatile suspended solid (VSS) per day, compared to 0.28 mmol CH4 per gram of VSS per day produced at 20°C. These results demonstrate that higher MFC operating temperatures could be detrimental to the biocatalytic performance of electrochemically active bacteria in anodic biofilms due to biomass accumulation with enhanced development of non-electrogenic communities (e.g. methanogens and fermenters), meaning that, over time, psychro- or mesophilic operation can have beneficial effects for the development of electrogenically active populations in the reactor.

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Acknowledgement

This research was funded by the RCUK Energy Programme, SUPERGEN Biological Fuel Cell project (EP/D047943/1 and EP/H019480/1). The Energy Programme is an RCUK cross-council initiative led by EPSRC and contributed to by ESRC, NERC, BBSRC and STFC.

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Correspondence to Iain S. Michie.

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Michie, I.S., Kim, J.R., Dinsdale, R.M. et al. Operational temperature regulates anodic biofilm growth and the development of electrogenic activity. Appl Microbiol Biotechnol 92, 419–430 (2011). https://doi.org/10.1007/s00253-011-3531-9

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Keywords

  • Microbial fuel cell (MFC)
  • Tubular reactor
  • Biofilm
  • Temperature
  • Electricity generation