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Production of oligosaccharides and cellobionic acid by Fibrobacter succinogenes S85 growing on sugars, cellulose and wheat straw

Abstract

Extracellular culture fluid of Fibrobacter succinogenes S85 grown on glucose, cellobiose, cellulose or wheat straw was analysed by 2D-NMR spectroscopy. Cellodextrins did not accumulate in the culture medium of cells grown on cellulose or straw. Maltodextrins and maltodextrin-1P were identified in the culture medium of glucose, cellobiose and cellulose grown cells. New glucose derivatives were identified in the culture fluid under all the substrate conditions. In particular, a compound identified as cellobionic acid accumulated at high levels in the medium of F. succinogenes S85 cultures. The production of cellobionic acid (and cellobionolactone also identified) was very surprising in an anaerobic bacterium. The results suggest metabolic shifts when cells were growing on solid substrate cellulose or straw compared to soluble sugars.

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Acknowledgements

MM was visiting professor of the University Blaise Pascal, Aubière, France. RN is grateful to the Région Auvergne, Centre National de la Recherche Scientifique and Institut National de la Recherche Agronomique for a Ph.D. grant. MM and VP were supported by VEGA 2/0108/08, APVV-0650-07 and the Slovak State Programme Project No. 2003SP200280203.

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Correspondence to Evelyne Forano.

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Nouaille, R., Matulova, M., Pätoprstý, V. et al. Production of oligosaccharides and cellobionic acid by Fibrobacter succinogenes S85 growing on sugars, cellulose and wheat straw. Appl Microbiol Biotechnol 83, 425–433 (2009). https://doi.org/10.1007/s00253-009-1884-0

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Keywords

  • Rumen
  • Fibrobacter succinogenes
  • Cellobionate
  • NMR
  • Oligosaccharides