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Biotechnological production of l-ribose from l-arabinose

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Abstract

l-Ribose is a rare and expensive sugar that can be used as a precursor for the production of l-nucleoside analogues, which are used as antiviral drugs. In this work, we describe a novel way of producing l-ribose from the readily available raw material l-arabinose. This was achieved by introducing l-ribose isomerase activity into l-ribulokinase-deficient Escherichia coli UP1110 and Lactobacillus plantarum BPT197 strains. The process for l-ribose production by resting cells was investigated. The initial l-ribose production rates at 39°C and pH 8 were 0.46 ± 0.01 g g−1 h−1 (1.84 ± 0.03 g l−1 h−1) and 0.27 ± 0.01 g g−1 h−1 (1.91 ± 0.1 g l−1 h−1) for E. coli and for L. plantarum, respectively. Conversions were around 20% at their highest in the experiments. Also partially purified protein precipitates having both l-arabinose isomerase and l-ribose isomerase activity were successfully used for converting l-arabinose to l-ribose.

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Acknowledgments

The authors thank Dr. Ossi Pastinen for excellent advice. Kalle Salonen, Marjaana Rytelä, and Auli Murrola are acknowledged for technical support. The research was funded by the Academy of Finland (210778).

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Correspondence to M. Helanto.

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Helanto, M., Kiviharju, K., Granström, T. et al. Biotechnological production of l-ribose from l-arabinose. Appl Microbiol Biotechnol 83, 77–83 (2009). https://doi.org/10.1007/s00253-008-1855-x

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Keywords

  • l-Ribose
  • l-Arabinose
  • Pentose metabolism
  • Metabolic engineering
  • Lactic acid bacteria