To develop a microbial treatment of edible oil-contaminated wastewater, microorganisms capable of rapidly degrading edible oil were screened. The screening study yielded a yeast coculture comprising Rhodotorula pacifica strain ST3411 and Cryptococcus laurentii strain ST3412. The coculture was able to degrade efficiently even at low contents of nitrogen ([NH4–N] = 240 mg/L) and phosphorus sources ([PO4–P] = 90 mg/L). The 24-h degradation rate of 3,000 ppm mixed oils (salad oil/lard/beef tallow, 1:1 w/w) at 20°C was 39.8% ± 9.9% (means ± standard deviations of eight replicates). The highest degradation rate was observed at 20°C and pH 8. In a scaled-up experiment, the salad oil was rapidly degraded by the coculture from 671 ± 52.0 to 143 ± 96.7 ppm in 24 h, and the degradation rate was 79.4% ± 13.8% (means ± standard deviations of three replicates). In addition, a repetitive degradation was observed with the cell growth by only pH adjustment without addition of the cells.
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Bednarski W, Adamczak M, Kowalewska-Piontas J, Zadernowski R (1994) Biotechnological methods for the up-grading and modification of animal waste fats. Acta Biotechnol 14:387–393
Kurtzman CP, Fell JW (1998) The Yeasts, a taxonomic study. Amsterdam, Netherlands: Elsevier
Matsumiya Y, Wakita D, Kimura A, Sanpa S, Kubo M (2007) Isolation and characterization of a lipid-degrading bacterium and its application to lipid-containing wastewater treatment. J Biosci Bioeng 103:325–330
Okuda S, Ito K, Ozawa H, Izaki K (1991) Treatment of lipid-containing wastewater using bacteria which assimilate lipids. J Ferment Bioeng 71:424–429
Sugimori D, Nakamura M, Mihara Y (2002) Microbial degradation of lipid by Acinetobacter sp. strain SOD-1. Biosci, Biotechnol, Biochem 66:1579–1582
Sugimoto Y, Maruoka T, Seo Y, Fujita M (1994) Microbe capable of degrading edible fatty oil and usage thereof. Japan Patent 06-153922
Suzuki T, Nakayama T, Kurihara T, Nishino T, Esaki N (2001) Cold-active lipolytic activity of psychrotrophic Acinetobacter sp. strain No. 6. J Biosci Bioeng 92:144–148
Taki H, Inoue R (2000) Microorganism having oil and fat-decomposing activity and treatment of waste water. Japan Patent 2000-270845
Wakelin NM, Forster CF (1997) An investigation into microbial removal of fats, oils and greases. Biores Technol 59:37–43
A part of this research was supported by the “Research for Promoting Technological Seeds” from Innovation Plaza Miyagi, Japan Science and Technology Agency.
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Sugimori, D. Edible oil degradation by using yeast coculture of Rhodotorula pacifica ST3411 and Cryptococcus laurentii ST3412. Appl Microbiol Biotechnol 82, 351–357 (2009). https://doi.org/10.1007/s00253-008-1834-2
- Oil degradation
- Wastewater treatment