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Diatoms in biotechnology: modern tools and applications


Diatoms have played a decisive role in the ecosystem for millions of years as one of the foremost set of oxygen synthesizers on earth and as one of the most important sources of biomass in oceans. Previously, diatoms have been almost exclusively limited to academic research with little consideration of their practical uses beyond the most rudimentary of applications. Efforts have been made to establish them as decisively useful in such commercial and industrial applications as the carbon neutral synthesis of fuels, pharmaceuticals, health foods, biomolecules, materials relevant to nanotechnology, and bioremediators of contaminated water. Progress in the technologies of diatom molecular biology such as genome projects from model organisms, as well as culturing conditions and photobioreactor efficiency, may be able to be combined in the near future to make diatoms a lucrative source of novel substances with widespread relevance.

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  1. Al-Degs Y, Khraisheh MA, Tutunji MF (2001) Sorption of lead ions on diatomite and manganese oxides modified diatomite. Water Res 35:3724–3728

  2. Allen JT, Brown L, Sanders R, Moore CM, Mustard A, Fielding S, Lucas M, Rixen M, Savidge G, Henson S, Mayor D (2005) Diatom carbon export enhanced by silicate upwelling in the northeast Atlantic. Nature 437:728–732

  3. Apt KE, Kroth-Pancic PG, Grossman AR (1996) Stable nuclear transformation of the diatom Phaeodactylum tricornutum. Mol Gen Genet 252:572–579

  4. Apt KE, Zaslavkaia L, Lippmeier JC, Lang M, Kilian O, Wetherbee R, Grossman AR, Kroth PG (2002) In vivo characterization of diatom multipartite plastid targeting signals. J Cell Sci 115:4061–4069

  5. Armbrust EV, Berges JA, Bowler C, Green BR, Martinez D, Putnam NH, Zhou S, Allen AE, Apt KE, Bechner M, Brzezinski MA, Chaal BK, Chiovitti A, Davis AK, Demarest MS, Detter JC, Glavina T, Goodstein D, Hadi MZ, Hellsten U, Hildebrand M, Jenkins BD, Jurka J, Kapitonov VV, Kroger N, Lau WW, Lane TW, Larimer FW, Lippmeier JC, Lucas S, Medina M, Montsant A, Obornik M, Parker MS, Palenik B, Pazour GJ, Richardson PM, Rynearson TA, Saito MA, Schwartz DC, Thamatrakoln K, Valentin K, Vardi A, Wilkerson FP, Rokhsar DS (2004) The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. Science 306:79–86

  6. Becker EW (2007) Micro-algae as a source of protein. Biotechnol Adv 25:207–210

  7. Bidle KD, Manganelli M, Azam F (2002) Regulation of oceanic silicon and carbon preservation by temperature control on bacteria. Science 298:1980–1984

  8. Bismuto A, Setaro A, Maddalena P, Stefano LD, Stefano MD (2008) Marine diatoms as optical chemical sensors: a time-resolved study. Sens Actuators B, Chem 130:396–399

  9. Cavalier-Smith T (2000) Membrane heredity and early chloroplast evolution. Trends Plant Sci 5:174–182

  10. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306

  11. Cobbett C, Goldsbrough P (2002) Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Annu Rev Plant Biol 53:159–182

  12. De Martino A, Meichenin A, Shi J, Pan K, Bowler C (2007) Genetic and phenotypic characterization of Phaeodactylum tricornutum (Bacillariophyceae) accessions. J Phycol 43:992–1009

  13. Delhaize E, Ryan PR (1995) Aluminum toxicity and tolerance in plants. Plant Physiol 107:315–321

  14. Drum RW, Gordon R (2003) Star trek replicators and diatom nanotechnology. Trends Biotechnol 21:325–328

  15. Dunahey T, Jarvis E, Roessler P (1995) Genetic transformation of the diatoms Cyclotella cryptica and Navicula saprophila. J Phycol 31:1004–1012

  16. Eriksen NT (2008) The technology of microalgal culturing. Biotechnol Lett 30:1525–1536

  17. Falciatore A, Bowler C (2002) Revealing the molecular secrets of marine diatoms. Annu Rev Plant Biol 53:109–130

  18. Falciatore A, Casotti R, Leblanc C, Abrescia C, Bowler C (1999) Transformation of nonselectable reporter genes in marine diatoms. Mar Biotechnol (NY) 1:239–251

  19. Falkowski PG, Barber RT, Smetacek VV (1998) Biogeochemical controls and feedbacks on ocean primary production. Science 281:200–207

  20. Field CB, Behrenfeld MJ, Randerson JT, Falkowski P (1998) Primary production of the biosphere: integrating terrestrial and oceanic components. Science 281:237–240

  21. Fischer H, Robl I, Sumper M, Kröger N (1999) Targeting and covalent modification of cell wall and membrane proteins heterologously expressed in the diatom Cylindrotheca fusiformis (Bacillariophyceae). J Phycol 35:113–120

  22. Gibbs SP (1979) The route of entry of cytoplasmically synthesized proteins into chloroplasts of algae possessing chloroplast ER. J Cell Sci 35:253–266

  23. Gilmore S, Weston P, Thomson J (1993) A simple, rapid, inexpensive and widely applicable technique for purifying plant DNA. Aust Syst Bot 6:139–142

  24. Gordon JM, Polle JE (2007) Ultrahigh bioproductivity from algae. Appl Microbiol Biotechnol 76:969–975

  25. Gould SB, Sommer MS, Hadfi K, Zauner S, Kroth PG, Maier UG (2006a) Protein targeting into the complex plastid of cryptophytes. J Mol Evol 62:674–681

  26. Gould SB, Sommer MS, Kroth PG, Gile GH, Keeling PJ, Maier UG (2006b) Nucleus-to-nucleus gene transfer and protein retargeting into a remnant cytoplasm of cryptophytes and diatoms. Mol Biol Evol 23:2413–2422

  27. Gould SB, Waller RF, Mcfadden GI (2008) Plastid evolution. Annu Rev Plant Biol 59:491–517

  28. Grill E, Winnacker EL, Zenk MH (1985) Phytochelatins: the principal heavy-metal complexing peptides of higher plants. Science 230:674–676

  29. Hempel F, Bozarth A, Sommer MS, Zauner S, Przyborski JM, Maier UG (2007) Transport of nuclear-encoded proteins into secondarily evolved plastids. Biol Chem 388:899–906

  30. Hildebrand M, Doktycz MJ, Allison DP (2008) Application of AFM in understanding biomineral formation in diatoms. Pflugers Arch 456:127–137

  31. Janech MG, Krell A, Mock T, Kang J-S, Raymond JA (2006) Ice-binding proteins from sea ice diatoms (Bacillariophyceae). J Phycol 42:410–416

  32. Jia Y, Hana W, Xionga G, Yanga W (2007) Diatomite as high performance and environmental friendly catalysts for phenol hydroxylation with H2O2. Sci Technol Adv Mater 8:106–109

  33. Kilian O, Kroth PG (2005) Identification and characterization of a new conserved motif within the presequence of proteins targeted into complex diatom plastids. Plant J 41:175–183

  34. Kroth P (2007) Molecular biology and the biotechnological potential of diatoms. Springer, Berlin

  35. Kroth PG, Chiovitti A, Gruber A, Martin-Jezequel V, Mock T, Parker MS, Stanley MS, Kaplan A, Caron L, Weber T, Maheswari U, Armbrust EV, Bowler C (2008) A model for carbohydrate metabolism in the diatom Phaeodactylum tricornutum deduced from comparative whole genome analysis. PLoS One 3:e1426

  36. Lang M, Apt KE, Kroth PG (1998) Protein transport into “complex” diatom plastids utilizes two different targeting signals. J Biol Chem 273:30973–30978

  37. Lebeau T, Robert JM (2003) Diatom cultivation and biotechnologically relevant products. Part I: cultivation at various scales. Appl Microbiol Biotechnol 60:612–623

  38. Lopez PJ, Descles J, Allen AE, Bowler C (2005) Prospects in diatom research. Curr Opin Biotechnol 16:180–186

  39. Maier UG, Douglas SE, Cavalier-Smith T (2000) The nucleo morph genomes of cryptophytes and chlorarachniophytes. Protist 151:103–109

  40. Meiser A, Schmid-Staiger U, Trösch W (2004) Optimization of eicosapentaenoic acid production by Phaeodactylum tricornutum in the flat panel airlift (FPA) reactor. J Appl Phycol 16:215–225

  41. Mock T, Samanta P, Iverson V, Berthiaume C, Robison M, Holtermann K, Durkin C, Bondurant S, Richmond K, Rodesch M, Kallas T, Huttlin E, Cerrina F, Sussman M, Armbrust EV (2007) Whole-genome expression profiling of the marine diatom Thalassiosira pseudonana identifies genes involved in silicon bioprocesses. PNAS 105:1579–1584

  42. Mussgnug JH, Thomas-Hall S, Rupprecht J, Foo A, Klassen V, Mcdowall A, Schenk PM, Kruse O, Hankamer B (2007) Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion. Plant Biotechnol J 5:802–814

  43. Nigh R, Benbrook C, Brush S, Garcia-Barrios L, Ortega-Paczka R, Perales HR (2000) Transgenic crops: a cautionary tale. Science 287:1927

  44. Parkinson J, Brechet Y, Gordon R (1999) Centric diatom morphogenesis: a model based on a DLA algorithm investigating the potential role of microtubules. Biochim Biophys Acta 1452:89–102

  45. Pérez-Caberoa M, Puchola V, Beltrána D, Amorós P (2008) Thalassiosira pseudonana diatom as biotemplate to produce a macroporous ordered carbon-rich material. Carbon 46:297–304

  46. Poulsen N, Kroger N (2005) A new molecular tool for transgenic diatoms: control of mRNA and protein biosynthesis by an inducible promoter-terminator cassette. FEBS J 272:3413–3423

  47. Poulsen N, Chesley P, Kröger N (2006) Molecular genetic manipulation of the diatom Thalassiosira pseudonana (Bacillariophyceae). J Phycol 42:1059–1065

  48. Prasad M, Freitas H (2003) Metal hyperaccumulation in plants—biodiversity prospecting for phytoremediation technology. Electron J Biotechnol 93:285–321

  49. Round FE, Crawford RM, Mann DG (2000) Diatoms: biology and morphology of the genera. Cambridge University Press, Cambridge, England

  50. Sakaue K, Harada H, Matsuda Y (2008) Development of gene expression system in a marine diatom using viral promoters of a wide variety of origin. Physiol Plant 133:59–67

  51. Salt DE, Blaylock M, Kumar NP, Dushenkov V, Ensley BD, Chet I, Raskin I (1995) Phytoremediation: a novel strategy for the removal of toxic metals from the environment using plants. Biotechnology (NY) 13:468–474

  52. Salt DE, Smith RD, Raskin I (1998) Phytoremediation. Annu Rev Plant Physiol Plant Mol Biol 49:643–668

  53. Sheehan J, Dunahay T, Benemann J, Roessler P (1998) A look back at the U.S. Department of energy’s aquatic species program: biodiesel from algae

  54. Sommer MS, Gould SB, Lehmann P, Gruber A, Przyborski JM, Maier UG (2007) Der1-mediated preprotein import into the periplastid compartment of chromalveolates? Mol Biol Evol 24:918–928

  55. Toppi LSD, Gabbrielli R (1999) Response to cadmium in higher plants. Environ Exp Bot 41:105–130

  56. Weatherspoon MR, Allan SM, Hunt E, Cai Y, Sandhage KH (2005) Sol–gel synthesis on self-replicating single-cell scaffolds: applying complex chemistries to nature’s 3-D nanostructured templates. Chem Commun 5:651–653

  57. Wee KM, Rogers TN, Altan BS, Hackney SA, Hamm C (2005) Engineering and medical applications of diatoms. J Nanosci Nanotechnol 5:88–91

  58. Wen ZY, Chen F (2001) A perfusion-cell bleeding culture strategy for enhancing the productivity of eicosapentaenoic acid by Nitzschia laevis. Appl Microbiol Biotechnol 57:316–322

  59. Wolfenbarger LL, Phifer PR (2000) The ecological risks and benefits of genetically engineered plants. Science 290:2088–2093

  60. Zaslavkaia L, Lippmeier C, Kroth P, Grossman A, Apt KE (2000) Transformation of the diatom Phaeodactylum tricornutum (Bacillariophyceae) with a variety of selectable marker and reporter genes. J Phycol 36:379–386

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Our work is support by the Deutsche Forschungsgemeinschaft (Graduate School 1216 AB, SZ; DFG Priority Program SFB593 UGM).

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Correspondence to Andrew Bozarth.

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Bozarth, A., Maier, U. & Zauner, S. Diatoms in biotechnology: modern tools and applications. Appl Microbiol Biotechnol 82, 195–201 (2009).

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  • Diatom
  • Nanotechnology
  • Biofuel
  • Bioremediation