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Cell-surface modification of non-GMO without chemical treatment by novel GMO-coupled and -separated cocultivation method

Abstract

We developed a novel method to coat living non-genetically modified (GM) cells with functional recombinant proteins. First, we prepared GM yeast to secrete constructed proteins that have two domains: a functional domain and a binding domain that recognizes other cells. Second, we cocultivated GM and non-GM yeasts that share and coutilize the medium containing recombinant proteins produced by GM yeasts using a filter-membrane-separated cultivation reactor. We confirmed that GM yeast secreted enhanced green fluorescent protein (EGFP) fusion proteins to culture medium. After cocultivation, EGFP fusion proteins produced by GM yeast were targeted to non-GM yeast (Saccharomyces cerevisiae BY4741ΔCYC8 strain) cell surface. Yeast cell-surface engineering is a useful method that enables the coating of GM yeast cell surface with recombinant proteins to produce highly stable and accumulated protein particles. The results of this study suggest that development of cell-surface engineering from GM organisms (GMOs) to living non-GMOs by our novel cocultivation method is possible.

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Correspondence to Mitsuyoshi Ueda.

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Miura, N., Aoki, W., Tokumoto, N. et al. Cell-surface modification of non-GMO without chemical treatment by novel GMO-coupled and -separated cocultivation method. Appl Microbiol Biotechnol 82, 293–301 (2009). https://doi.org/10.1007/s00253-008-1787-5

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Keywords

  • Non-GMO (genetically modified organism)
  • Cocultivation
  • Cell-surface engineering
  • MSSM (molecular sniping and shooting method)