The two homologous genes GPD1 and GPD2, encoding two isoenzymes of NAD+-dependent glycerol-3-phosphate dehydrogenase in industrial yeast Saccharomyces cerevisiae CICIMY0086, had been deleted. The obtained two kinds of mutants gpd1Δ and gpd2Δ were studied under alcoholic fermentation conditions. gpd1Δ mutants exhibited a 4.29% (relative to the amount of substrate consumed) decrease in glycerol production and 6.83% (relative to the amount of substrate consumed) increased ethanol yield while gpd2Δ mutants exhibited a 7.95% (relative to the amount of substrate consumed) decrease in glycerol production and 7.41% (relative to the amount of substrate consumed) increased ethanol yield compared with the parental strain. The growth rate of the two mutants were slightly lower than that of the wild type under the exponential phase whereas ANG1 (gpd1Δ) and the decrease in glycerol production was not accompanied by any decline in the protein content of the strain ANG1 (gpd1Δ) but a slight decrease in the strain ANG2 (gpd2Δ). Meanwhile, dramatic decrease of acetate acid formation was observed in strain ANG1 (gpd1Δ) and ANG2 (gpd2Δ) compared to the parental strain. Therefore, it is possible to improve the ethanol yield by interruption of glycerol pathway in industrial alcoholic yeast.
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This work was financial supported by the Natural Science Foundation of China (20706024), the “863” Program(2006AA020101, 2007AA10Z359), the Open Project Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (KLIB-KF200701) and Technology Development Programme for institute of Yunnan Province (No.2006KFZX-10).
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Guo, Z., Zhang, L., Ding, Z. et al. Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production. Appl Microbiol Biotechnol 82, 287–292 (2009). https://doi.org/10.1007/s00253-008-1777-7
- Saccharomyces cerevisiae
- Glycerol-3-phosphate dehydrogenase
- Glycerol production
- Ethanol yield