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Long-term production of soluble human Fas ligand through immobilization of Dictyostelium discoideum in a fibrous bed bioreactor

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The production of recombinant glycoproteins in Dictyostelium discoideum by conventional cell culture methods was limited by low cell density as well as low growth rate. In this work, cotton towel with a good adsorption capability for D. discoideum cells was used as the immobilization matrix in an external fibrous bed bioreactor (FBB) system. With batch cultures in the FBB, the concentration of immobilized cells in the cotton fiber carrier increased to 1.37 × 108 cells per milliliter after 110-h cultivation, which was about tenfold higher than the maximal cell density in the conventional free-cell culture. Correspondingly, a high concentration of soluble human Fas ligand (hFasL; 173.7 μg l−1) was achieved with a high productivity (23 μg l−1 h−1). The FBB system also maintained a high density of viable cells for hFasL production during repeated-batch cultures, achieving a productivity of 9∼10 μg l−1 h−1 in all three batches studied during 15 days. The repeated-batch culture using immobilized cells of D. discoideum in the FBB system thus provides a good method for long-term and high-level production of hFasL.

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This work was financially supported by the National Natural Science Foundation of China (nos. 20736008, 30370039, and 20676115), the National Basic Research Program of China (2007CB707805), and the Ministry of Science and Technology, China.

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Correspondence to Zhinan Xu.

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Chen, J., Chen, H., Zhu, X. et al. Long-term production of soluble human Fas ligand through immobilization of Dictyostelium discoideum in a fibrous bed bioreactor. Appl Microbiol Biotechnol 82, 241–248 (2009). https://doi.org/10.1007/s00253-008-1769-7

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  • Dictyostelium discoideum
  • Immobilization
  • Soluble human Fas ligand
  • Fibrous bed bioreactor