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A new strain of Streptomyces avermitilis produces high yield of oligomycin A with potent anti-tumor activity on human cancer cell lines in vitro

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A new actinomycete strain, isolated from soil in China, strongly inhibited in vitro proliferation of human hepatoma, chronic myelogenous leukemia, and colonic carcinoma cell lines. The strain, designated L033, was identified as a strain of Streptomyces avermitilis based on cultural property, morphology, carbon source utilization, 16s rRNA gene analysis, and DNA–DNA relatedness studies. The anticancer component from L033 was purified to homogeneity by preparative positive-phase high-performance liquid chromatography and crystallization. Nuclear magnetic resonance and mass spectrometric analysis showed that this compound had the same structure as oligomycin A. Different with other reported naturally occurring strains of S. avermitilis, L033 produced high quantity of oligomycin A (maximal 1,461 μg/ml). Therefore, L033 was considered of great potential as an industrial oligomycin-A-producing strain.

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This study was supported by grants from the National Basic Research Program of China (grant no. 2003CB114205) and the National High Technology Research and Development Program (grant no. 2006AA10A209). Anti-tumor activities of oligomycin A in vitro were determined by the National Center for Pharmaceutical Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College. We are grateful to Han Bing (Beijing Institute of Biomedicine) for structural elucidation of the compound.

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Correspondence to Jilun Li.

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Fig. S1

1H-NMR spectrum of compound 1 (CDCl3). (DOC 419 KB)

Fig. S2

13C-NMR spectrum of compound 1 (CDCl3). (DOC 1.10 MB)

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Lin, X., Wen, Y., Li, M. et al. A new strain of Streptomyces avermitilis produces high yield of oligomycin A with potent anti-tumor activity on human cancer cell lines in vitro. Appl Microbiol Biotechnol 81, 839–845 (2009).

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  • Streptomyces avermitilis
  • Oligomycin A
  • Anti-tumor activity