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Autocatalysis in Reactive Black 5 biodecolorization by Rhodopseudomonas palustris W1

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Abstract

Autocatalysis in biological decolorization of Reactive Black 5 (RB5) by Rhodopseudomonas palustris W1 was investigated in batch assays. An improvement of 1.5-fold in decolorization rate of RB5 was obtained by supplementing decolorization metabolites from 200 mg l−1 RB5. Liquid chromatography-mass spectrometry and cyclic voltammetric analysis revealed that the constituent of dye precursors, from azo bonds breakage, with quinone-like structure and reversible oxidation–reduction activity can be used as redox mediators and was responsible for the catalytic reduction of RB5. The required amount of metabolites for catalytic decolorization was quite small, indicating its possible application in real textile wastewater treatment. Furthermore, decolorization metabolites of RB5 were shown as effective in catalyzing anaerobic decolorization of Direct Yellow 11, an azo dye without autocatalyic activity.

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Acknowledgments

This work was supported by the National Key Basic Research Program of China (973 Program, Grant No. 2004CB418505) and the Foundation for Excellent Youth of Heilongjiang Province of China.

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Correspondence to Dezhi Sun.

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Wang, X., Cheng, X. & Sun, D. Autocatalysis in Reactive Black 5 biodecolorization by Rhodopseudomonas palustris W1. Appl Microbiol Biotechnol 80, 907–915 (2008). https://doi.org/10.1007/s00253-008-1657-1

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Keywords

  • Catalytic decolorization
  • Reactive Black 5
  • Redox mediator
  • Rhodopseudomonas palustris