Applied Microbiology and Biotechnology

, Volume 80, Issue 5, pp 873–880 | Cite as

Growth of Salinispora tropica strains CNB440, CNB476, and NPS21184 in nonsaline, low-sodium media

  • Ginger Tsueng
  • Kin S. LamEmail author
Applied Microbial and Cell Physiology


We recently described the development of a potassium-chloride-based salt formulation containing low sodium concentration (5.0 mM) to support the growth of Salinispora tropica strain NPS21184 and its production of salinosporamide A (NPI-0052). In order to determine whether the above low-sodium salt formulation can also support the growth of other S. tropica strains, we examined the growth of the type strain CNB440 and the parent strain CNB476, from which strain NPS21184 was derived as a single colony isolate. We demonstrated that good growth rate and yield of S. tropica strains CNB440 and CNB476, similar to S. tropica strain NPS21184 reported earlier, were detected in both agar and liquid media containing the potassium-chloride-based salt formulation with sodium concentration of 5.0 mM. Furthermore, we also detected good growth rate and yield of all three S. tropica strains on potassium-sulfate-based salt formulation agar medium containing both low-sodium (5.7 mM) and low-chloride (14 mM) content. This finding confirms the observation that the species of S. tropica does not have a seawater growth requirement but requirement for a specific combination of salts to provide a balance of salts and maintain a high enough ionic strength for growth.


Salinosporamide A NPI-0052 Salinispora tropica Low-sodium salt formulation Nonsaline fermentation Marine actinomycete 


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Nereus PharmaceuticalsSan DiegoUSA

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