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Deep-sea thermophilic Geobacillus bacteriophage GVE2 transcriptional profile and proteomic characterization of virions

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Abstract

Thermophilic bacteria and viruses represent novel sources of genetic materials and enzymes with great potential for use in industry and biotechnology. In this study, GVE2, a virulent tailed Siphoviridae bacteriophage infecting deep-sea thermophilic Geobacillus sp. E263, was characterized. The bacteriophage contained a 40,863-bp linear double-stranded genomic deoxyribonucleic acid (DNA) with 62 presumptive open reading frames (ORFs). A viral DNA microarray was developed to monitor the viral gene transcription program. Microarray analysis indicated that 74.2% of the presumptive ORFs were expressed. The structural proteins of purified GVE2 virions were identified by mass spectrometric analysis. The purified virions contained six protein bands. Of the newly retrieved proteins, VP371 was further characterized. The immuno-electron microscopy indicated that the VP371 protein was a component of the viral capsid. Transcriptional analyses and proteomic characterization of GVE2 would be helpful to understand the complex host–virus interaction during virus infection.

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Acknowledgments

This work was financially supported by China Ocean Mineral Resources R & D Association (DYXM-115-02-2-15), National Natural Science Foundation of China (40576076), and Hi-Tech Research and Development Program of China (863 program of China; 2007AA091407).

Author information

Correspondence to Xiaobo Zhang.

Appendix

Appendix

Table 1 Viral DNA fragments spotted on the microarray
Table 2 DNA microarray
Table 3 Listing of potentially expressed ORFs in GVE2

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Liu, B., Zhang, X. Deep-sea thermophilic Geobacillus bacteriophage GVE2 transcriptional profile and proteomic characterization of virions. Appl Microbiol Biotechnol 80, 697–707 (2008). https://doi.org/10.1007/s00253-008-1575-2

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Keywords

  • Thermophilic Geobacillus
  • Bacteriophage
  • Transcription
  • Proteomics