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Production of l-phenylacetylcarbinol by microbial transformation in polyethylene glycol-induced cloud point system

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Microbial transformation of benzaldehyde into l-phenylacetylcarbinol by whole cell Saccharomyces cerevisiae has been carried out in a novel polyethylene glycol (PEG)-induced cloud point system. The system is composed of 80 g PEG 20,000, 75 ml Triton X-100, 20 g peptone, 10 g yeast extract, 25 g glucose, 1 g MgSO4·7H2O, 0.05 g CaCl2·2H2O, 35 g Na2HPO4·12H2O, and 10.7 g citric acid per liter of tap water. The microbial transformation is conducted at 0.6 ml of acetaldehyde (35% volume content), 0.9 ml of benzaldehyde, and 7 g of wet cell per 100 ml of the PEG-induced cloud point system. Under the conditions, a relatively longer-term bioactivity of whole cell microorganism in the PEG-induced cloud point system has been achieved. A fed-batch microbial transformation process with a discrete addition of glucose and substrate gets a high final product concentration of about 8 g/l.

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This project was financially supported by the National Natural Science Foundation of China (no. 20676080).

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Correspondence to Zhilong Wang.

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Zhang, W., Wang, Z., Li, W. et al. Production of l-phenylacetylcarbinol by microbial transformation in polyethylene glycol-induced cloud point system. Appl Microbiol Biotechnol 78, 233–239 (2008).

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  • Microbial transformation
  • l-phenylacetylcarbinol
  • Polyethylene glycol
  • Nonionic surfactant
  • Cloud point system