Applied Microbiology and Biotechnology

, Volume 78, Issue 2, pp 319–331 | Cite as

Oleic acid delays and modulates the transition from respiratory to fermentative metabolism in Saccharomyces cerevisiae after exposure to glucose excess

  • David Feria-Gervasio
  • Jean-Roch Mouret
  • Nathalie Gorret
  • Gérard Goma
  • Stéphane E. GuillouetEmail author
Applied Microbial and Cell Physiology


This work aimed to study the transition from respiratory to fermentative metabolism in Saccharomyces cerevisiae CEN.PK 113-7D and more specifically to evaluate the implication of the acetyl-coenzymeA-derived carbon transport from cytosol to mitochondria in the onset of the metabolic shift. The strategy consisted in introducing, during aerobic glucose-limited chemostat (D = 0.16 h1), a local perturbation around the step to be studied by the addition of cosubstrate and in analyzing the consequences of such a perturbation on the metabolic transition. Oleic acid and l-carnitine were among the tested cosubstrates because they were known to stimulate enzymes implicated in the acetyl-coenzymeA transport between the different cell compartments, such as the carnitine acetyl transferases. The metabolic transition was then comparatively quantified in sole glucose and in glucose/oleic acid chemostats in presence/absence of l-carnitine after a pulse of glucose. Feeding the culture with oleic acid (Dole = 0.0041 and 0.0073 h1) led to a delay in the onset of the metabolic shift (up to 15 min), a 33% decrease in the ethanol production and a redirection of the carbon flux toward biomass production. The data clearly showed a modulation of the carbon distribution among respiration and fermentation, in favor of a decrease in the “short-term” Crabtree effect by the oleic acid.


Crabtree effect Saccharomyces Ethanolic fermentation Oleic acid Carnitine Carnitine acetyl transferase 



We thank Prof. J. Nielsen for giving us the opportunity to carry out isotopic labeling experiment in his laboratory at T.U. Lyngby (Denmark) and for his helpful discussion. J.R. Mouret was supported by a doctoral grant from the French Ministry of Education and Research and during his stay in Lyngby from the Federation of European Microbiological Societies. D. Feria-Gervasio gratefully acknowledges financial doctoral support by the Conacyt (Mexico).


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • David Feria-Gervasio
    • 1
  • Jean-Roch Mouret
    • 1
  • Nathalie Gorret
    • 1
  • Gérard Goma
    • 1
  • Stéphane E. Guillouet
    • 1
    Email author
  1. 1.UMR5504, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, CNRS, INRA, INSAToulouse CedexFrance

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