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Bacillus circulans WZ-12—a newly discovered aerobic dichloromethane-degrading methylotrophic bacterium

Abstract

A novel dichloromethane (DCM)-degrading bacterial strain named WZ-12 (GenBank accession no. EF100968) was isolated and identified as Bacillus circulans based on standard morphological and physiological properties and nucleotide sequence analysis of enzymatically amplified 16S ribosomal deoxyribonucleic acid. DCM dehalogenase from B. circulans WZ-12 was purified to 8.27-fold with a yield of 34.83%. The electrophoretically homogeneous-purified enzyme exhibited a specific activity of 118.82 U/mg. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis of purified DCM dehalogenase gave a distinct band with an estimated molecular mass of 20,000 ± 1,000.

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Acknowledgments

This research work was sponsored by the Natural Science Foundations of China and Zhejiang Province (20276070, 20476099, 2056124, Y304091).

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Correspondence to J. M. Chen.

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Wu, S.J., Zhang, L.L., Wang, J.D. et al. Bacillus circulans WZ-12—a newly discovered aerobic dichloromethane-degrading methylotrophic bacterium. Appl Microbiol Biotechnol 76, 1289–1296 (2007). https://doi.org/10.1007/s00253-007-1100-z

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Keywords

  • Dichloromethane
  • Bacillus circulans
  • Dehalogenase
  • Biodegradation