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Characterization of estrogen-degrading bacteria isolated from an artificial sandy aquifer with ultrafiltered secondary effluent as the medium

Abstract

This study investigated the aerobic and anoxic biodegradation of four estrogens [estrone (E1), estradiol (E2), estriol (E3), and the synthetic 17α-ethinylestradiol (EE2)] in microcosms constructed with marine sand and ulftrafiltered (UF) secondary effluent. Three estrogen-degrading bacteria, LHJ1, LHJ3, and CYH, were isolated. Based on gram-stain morphology and 16S rRNA sequence homology, LHJ1 and LHJ3 belong to the genus Acinetobacter and Agromyces, respectively; CYH matched to 95% with the genus Sphingomonas. Aerobically LHJ3 degrades E3, CYH degrades E1, and all three isolates oxidize E2 to E1. Under anoxic conditions, CYH degrades E1 and LHJ3 degrades E2, whereas E3 and EE2 were not degraded by the three isolates; EE2 was transformed in microcosms incubated with site ground water. The degradation kinetics of E1 and E2 by CYH and E2 by LHJ3 under aerobic conditions was linearly correlated with the initial concentration, which ranged from 50 to 2,000 μg/l. The degradation of E1 by CYH under anoxic conditions followed Michaelis–Menten kinetics. 16α-Hydroxyestrone was found to be a transient transformation product of E3 under aerobic conditions.

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References

  1. Abdel-EI-Hallem D (2003) Acinetobacter: environmental and biotechnological applications. Afr J Biotechnol 2:71–74

  2. Ahmad S, Shuy EB, Ngonidzashe M, Kem CM (2001) A preliminary study on feasibility of using treated wastewater to recharge reclaimed land. XXXIX Iahr Congress Proceeding. Theme A: development, planing and management of surface and groundwater resource: 152. September 15–21, Beijing, China

  3. Barnoti C, Curini R, Ascenzo G, Corcia A, Samperi R (2000) Monitoring natural and synthetic estrogens at activated sludge sewage treatment plants and in a receiving river water. Environ Sci Technol 34:5059–5066

  4. Belfroid AC, Van der Horst A, Vethaak AD, Schafer AJ, Rijs GBJ, Wegener J, Cofino WP (1999) Analysis and occurrence of estrogenic hormones and their glucuronides in surface water and waste water in The Netherlands. Sci Total Environ 225:101–108

  5. Berg UT, Nyholm N (1996) Biodegradability simulation studies in semicontinuous activated sludge reactors with low (microgram/l range) and standard (ppm range) chemical concentrations. Chemosphere 33:711–735

  6. Colucci MS, Topp E (2001) Persistence of estrogenic hormones in agricultural soils: II. 17α-ethynylestradiol. J Environ Qual 30:2077–2080

  7. Drewes JE, Reinhard M, Fox P (2003) Comparing microfiltration-reverse osmosis and soil-aquifer treatment for indirect potable reuse of water. Water Res 37:3612–3621

  8. Fujii K, Kikuchi S, Satomi M, Ushio-Sata N, Morita N (2002) Degradation of 17β-estradiol by a gram-negative bacterium isolated from activated sludge in a sewage treatment plant in Tokyo, Japan. Appl Environ Microbiol 68:2057–2060

  9. Fujii K, Satomi M, Morita N, Motomura T, Tanaka T, Kikuchi S (2003) Novosphingobium tardaugens sp. nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo. Int J Syst Evol Microbiol 53:47–52

  10. Järvenpää P, Kosunen T, Fotsis T, Adlercreuta H (1980) In vitro metabolism of estrogens by isolated intestinal micro-organisms and by human faecal microflora. J Steroid Biochem 13:345–349

  11. Lane DJ (1991) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, United Kingdom

  12. Lee HB, Liu D (2002) Degradation of 17β-estradiol and its metabolites by sewage bacteria. Water Air Soil Pollut 134:351–366

  13. López de Alda MJ, Barceló D (2000) Determination of steroid sex hormones and related synthetic compounds considered as endocrine disrupters in water by liquid chromatography-diode array detection-mass spectrometry. J Chromatogr Libr 892:391–406

  14. Mash H, Fox P, Westerhoff P, Arnold R, Quanrud D, Littlehart P (2003). Estrogenicity and size fractionation of organic material during soil aquifer treatment of wastewater. 11th Biennial Symposium on the Water Recharge. Phoenix, AZ

  15. Shi JH, Suzuki Y, Lee BD, Nakai S, Hosomi M (2002) Isolation and characterization of the ethynylestradiol biodegrading microorganism Fusarium proliferatum strain HNS-1. Water Sci Technol 45:175–179

  16. Shi JH, Fujisawa S, Nakai S, Hosomi M (2004a) Biodegradation of natural and synthetic estrogens by nitrifying activated sludge and ammonia-oxidizing bacterium Nitrosomonas europaea. Water Res 38:2323–2330

  17. Shi JH, Suzuki Y, Nakai S, Hosomi M (2004b) Microbial degradation of estrogens using activated sludge and night soil-composting microorganisms. Water Sci Technol 50:153–159

  18. Sóle M, Lopez de Alda MJ, Castillo M, Porte C, Ladegaard-Pedersen K, Barcelo D (2000) Estrogenicity determination in sewage treatment plants and surface waters from the Catalonian area (NE Spain). Environ Sci Technol 34:5076–5083

  19. Ternes TA, Stumpf M, Mueller J, Haberer K, Wilken RD, Servos M (1999) Behavior and occurrence of estrogens in municipal sewage treatment plants—I. Investigations in Germany, Canada and Brazil. Sci Total Environ 225:81–90

  20. Vader JS, van Ginkel CG, Sperling FM, de Jong J, de Boer W, de Graaf JS, van der Most M, Stokman PG (2000) Degradation of ethinyl estradiol by nitrifying activated sludge. Chemosphere 41:1239–1243

  21. Ying GG, Kookana RS (2003) Degradation of five selected endocrine-disrupting chemicals in seawater and marine sediment. Environ Sci Technol 37:1256–1260

  22. Ying GG, Kookana RS, Dillon P (2003) Sorption and degradation of selected five endocrine disrupting chemicals in aquifer material. Water Res 37:3785–3791

  23. Yoshimoto T, Nagai F, Fujimoto J, Watanabe K, Mizukoshi H, Makino T, Kimura K, Saino H, Sawada H, Omura H (2004) Degradation of estrogens by Rhodococcus zopfii and Rhodococcus equi isolates from activated sludge in wastewater treatment plants. Appl Environ Microbiol 70:5283–5289

  24. Yu CP, Roh H, Chu KH (2007) 17β-Estradiol-degrading bacteria isolated from activated sludge. Environ Sci Technol 41(2):486–492

  25. Zhuang WQ, Tay JH, Maszenan A, Tay S (2002) Bacillus naphthovorans sp. nov. from oil-contaminated tropical marine sediments and its role in naphthalene biodegradation. Appl Microbiol Biotechnol 58:547–554

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Acknowledgments

This study was supported by the Agency for Science and Technology, Singapore (ASTAR) and Nanyang Technological University. We would like to thank Yuehua Chen, Jason Leong, and Lim Han Liew for the assistance in the isolation work and Shuang Xie and Tiong Gim Aw for their assistance in microbial counting.

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Correspondence to Karina Yew-Hoong Gin.

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Ke, J., Zhuang, W., Gin, K.Y. et al. Characterization of estrogen-degrading bacteria isolated from an artificial sandy aquifer with ultrafiltered secondary effluent as the medium. Appl Microbiol Biotechnol 75, 1163–1171 (2007). https://doi.org/10.1007/s00253-007-0923-y

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Keywords

  • Estrogens
  • Biodegradation
  • Bacteria
  • Isolation
  • Tropical aquifer