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A chimeric baculovirus displaying bovine herpesvirus-1 (BHV-1) glycoprotein D on its surface and their immunological properties

Abstract

The ability of a recombinant baculovirus containing the ectodomain of the mature sequence of glycoprotein D (gD) fused to the amino-terminus of baculoviral glycoprotein gp64 to display gD on its surface and to serve as an improved immunogen against bovine herpesvirus-1 was tested. The gD–gp64 fusion protein was correctly expressed on the virus particles as revealed by immunomicroscopy assays. Mice immunized with 5 × 108 plaque forming units developed antibodies that specifically reacted in an enzyme-linked immunosorbent assay with recombinant gD and whole bovine herpesvirus-1. These antibodies were able to neutralize bovine herpesvirus-1 in vitro, whereas those elicited by a version of gD expressed in Escherichia coli did not. Our data demonstrated that the display on the virion surface of recombinant baculovirus can provide a tool for the development of recombinant vaccines against bovine herpesvirus-1.

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References

  1. Abe T, Takahashi H, Hiroyuki Hamazaki, Miyano-Kurosaki N, Matsuura Y, Takaku H (2003) Baculovirus induces an innate immune response and confers protection from lethal influenza virus infection in mice. J Immunol 171:1133–1139

  2. Abe T, Hemmi H, Miyamoto H, Moriishi K, Tamura S, Takaku H, Akira S, Matsuura Y (2005) Involvement of the toll-like receptor 9 signaling pathway in the induction of innate immunity by baculovirus. J Virol 79:2847–2858

  3. Boublik Y, Di Bonito P, Jones IM (1995) Eukaryotic virus display: engineering the major surface glycoprotein of the Autographa californica nuclear polyhedrosis virus (AcNPV) for the presentation of foreign proteins on the virus surface. Biotechnology (NY) 13:1079–1084

  4. Boyce FM, Bucher NLR (1996) Baculovirus-mediated gene transfer into mammalian cells. Proc Natl Acad Sci USA 93:2348–2352

  5. Campadelli-Fiume G, Arsenakis M, Farabegoli F, Roizman B (1988) Entry of herpes simplex virus 1 in BJ cells that constitutively express viral glycoprotein D is by endocytosis and results in degradation of the virus. J Virol 62:159–167

  6. Chase CCL, Carter-Allen K, Lohff C, Letchworth GJ (1990) Bovine cells expressing bovine herpesvirus 1 (BHV-1) glycoprotein IV resist infection by BHV-1, herpes simplex virus, and pseudorabies virus. J Virol 64:4866–4872

  7. Dasika GK, Letchworth GJ 3rd (1999) Cellular expression of bovine herpesvirus 1 gD inhibits cell-to-cell spread of two closely related viruses without blocking their primary infection. Virology 254:24–36

  8. Dubuisson J, Israel BA, Letchworth GJ 3rd (1992) Mechanism of bovine herpesvirus type 1 neutraliztion by monoclonal antibodies to glycoproteins gI, gIII and gIV. J Gen Virol 73:2031–2039

  9. Francis MJ, Clarke BE (1989) Peptide vaccines based on enhanced immunogenicity of peptide epitopes presented with T-cell determinants of hepatitis B core protein. Meth Enzimol 178:659–673

  10. Gamvrellis A, Leong D, Hanley JC, Xiang SD, Mottram P, Plebanski M (2004) Vaccines that facilitate antigen entry into dendritic cells. Immunol Cell Biol 82:506–516

  11. Hughes G, Babiuk LA, van Drunen Littel-van den Hurk S (1988) Functional and topographical analysis of epitopes on bovine herpesvirus type 1 glycoprotein IV. Arch Virol 103:47–60

  12. Israel BA, Herber R, Gao Y, Letchworth GJ 3rd (1992) Induction of a mucosal barrier to bovine herpesvirus 1 replication in cattle. Virology 188:256–264

  13. Johnson DC, Ligas MW (1988) Herpes simplex viruses lacking glycoprotein D are unable to inhibit virus penetration, quantitative evidence for virus-specific cell surface receptors. J Virol 62:4605–4612

  14. Johnson DC, Burke RL, Gregory T (1990) Soluble forms of herpes simplex virus glycoprotein D bind to a limited number of cell surface receptors and inhibit virus entry into cells. J Virol 64:2569–2576

  15. Kaba SA, Hemmes JC, van Lent JW, Vlak JM, Nene V, Musoke AJ, van Oers MM (2003) Baculovirus surface display of Theileria parva p67 antigen preserves the conformation of sporozoite-neutralizing epitopes. Protein Eng 16:73–78

  16. Kowalski J, Gilvert SA, van Druen Littel-van den Hurk S, van den Hurk JV, Babiuk LA, Zamb TJ (1993) Heat-shock promoter driven synthesis of secreted bovine herpesvirus glycoproteins in transfected cells. Vaccine 11:1100–1108

  17. Maniatis T, Fritsch, EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor, New York

  18. Mottershead D, van der Linden I, von Bonsdorff CH, Keinanen K, Oker-Blom C (1997) Baculoviral display of the green fluorescent protein and rubella virus envelope proteins. Biochem Biophys Res Comm 238:717–722

  19. Noad R, Roy P (2003) Virus-like particles as immunogens. Trends Microbiol 11:438–444

  20. O’Reilly DR, Miller L, Luckow VA (1992) Baculovirus expression vectors. A laboratory manual. Freeman, New York

  21. Perez Filgueira DM, Zamorano PI, Dominguez MG, Taboga O, Del Medico Zajac MP, Puntel M, Romera SA, Morris TJ, Borca MV, Sadir AM (2003) Bovine herpes virus gD protein produced in plants using a recombinant tobacco mosaic virus (TMV) vector possesses authentic antigenicity. Vaccine 21:4201–4209

  22. Roy P (1996) Genetically engineered particulate virus-like structures and their use as vaccine delivery systems. Intervirology 39:62–71

  23. Tami C, Farber M, Palma EL, Taboga O (2000) Presentation of antigenic sites from foot-and-mouth disease virus on the surface of baculovirus and in the membrane of infected cells. Arch Virol 145:1815–1828

  24. Tobin GJ, Nagashima K, Gonda MA (1996) Synthesis and assembly of chimeric human immunodeficiency virus gag pseudovirions. Intervirology 39:40–48

  25. van Drunen Littel-van den Hurk S, Parker MD, Massie B, van den Hurk JV, Harland R, Babiuk LA, Zamb TJ (1993) Protection of cattle from BHV-1 infection by immunization with recombinant glycoprotein gIV. Vaccine 11:25–35

  26. van Drunen Littel-van den Hurk S, Van Donkersgoed J, Kowalski J, van den Hurk JV, Harland R, Babiuk LA, Zamb TJ (1994) A subunit gIV vaccine, produced by transfected mammalian cells in culture, induces mucosal immunity against bovine herpesvirus-1 in cattle. Vaccine 12:1295–1302

  27. Yates WD (1982) A review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral–bacterial synergism in respiratory disease of cattle. Can J Comp Med 46:225–263

  28. Yoshida S, Kondoh D, Arai E, Matsuoka H, Seki C, Tanaka T, Okada M, Ishii A (2003) Baculovirus virions displaying Plasmodium berghei circumsporozoite protein protect mice against malaria sporozoite infection. Virology 10:161–170

  29. Zhu X, Letchworth GJ (1996) Mucosal and systemic immunity to bovine herpesvirus-1 glycoprotein D confer resistance to viral replication and latency. Vaccine 14:61–69

  30. Zhu X, Wu S, Letchworth GJ (1997) Yeast-secreted bovine herpesvirus type1 glycoprotein D authentic conformational structure and immunogenicity. Vaccine 15:679–688

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Aknowledgment

Authors are grateful to María José Mónaco and Vilma Calderón for their invaluable technical assistance.

This work was supported in part by Grant PICT 98 3515 from the Agencia Nacional de Promoción Científica y Tecnológica.

A. P. and P. M. contributed equally to this work.

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Correspondence to O. Taboga.

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Peralta, A., Molinari, P., Conte-Grand, D. et al. A chimeric baculovirus displaying bovine herpesvirus-1 (BHV-1) glycoprotein D on its surface and their immunological properties. Appl Microbiol Biotechnol 75, 407–414 (2007). https://doi.org/10.1007/s00253-006-0825-4

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Keywords

  • Baculovirus display
  • BHV-1 vaccines
  • Viral vectors