The ability of a recombinant baculovirus containing the ectodomain of the mature sequence of glycoprotein D (gD) fused to the amino-terminus of baculoviral glycoprotein gp64 to display gD on its surface and to serve as an improved immunogen against bovine herpesvirus-1 was tested. The gD–gp64 fusion protein was correctly expressed on the virus particles as revealed by immunomicroscopy assays. Mice immunized with 5 × 108 plaque forming units developed antibodies that specifically reacted in an enzyme-linked immunosorbent assay with recombinant gD and whole bovine herpesvirus-1. These antibodies were able to neutralize bovine herpesvirus-1 in vitro, whereas those elicited by a version of gD expressed in Escherichia coli did not. Our data demonstrated that the display on the virion surface of recombinant baculovirus can provide a tool for the development of recombinant vaccines against bovine herpesvirus-1.
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Authors are grateful to María José Mónaco and Vilma Calderón for their invaluable technical assistance.
This work was supported in part by Grant PICT 98 3515 from the Agencia Nacional de Promoción Científica y Tecnológica.
A. P. and P. M. contributed equally to this work.
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Peralta, A., Molinari, P., Conte-Grand, D. et al. A chimeric baculovirus displaying bovine herpesvirus-1 (BHV-1) glycoprotein D on its surface and their immunological properties. Appl Microbiol Biotechnol 75, 407–414 (2007). https://doi.org/10.1007/s00253-006-0825-4
- Baculovirus display
- BHV-1 vaccines
- Viral vectors