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Screening of β-fructofuranosidase-producing microorganisms and effect of pH and temperature on enzymatic rate

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Seventeen different strains of filamentous fungi were grown in batch cultures to compare their abilities for the production of β-fructofuranosidase. Three of them, Aspergillus oryzae IPT-301, Aspergillus niger ATCC 20611 and strain IPT-615, showed high production with total fructosyltransferase activity higher than 12,500 units l−1. In addition, the β-fructofuranosidases of those strains have a high fructosyltransferase activity-to-hydrolytic activity ratio. The temperature and pH effects on the sucrose-β-fructofuranosidase reaction rate were studied using a 22 factorial experimental design. The comparative analysis of the tested variable coefficients shows that the variable pH contributes mostly to the changes in the fructosyltransferase and hydrolytic rates and in the V t/V h ratio. At 40 and 50°C, there were no significant differences between the fructosyltransferase and hydrolytic velocities of these enzymes.

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Authors gratefully acknowledge The State of São Paulo Research Foundation (FAPESP) for providing financial support. We thank Dr. Antonio Bonomi for critical review of the manuscript.

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Correspondence to Maria Filomena de Andrade Rodrigues.

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Fernandez, R.C., Ottoni, C.A., da Silva, E.S. et al. Screening of β-fructofuranosidase-producing microorganisms and effect of pH and temperature on enzymatic rate. Appl Microbiol Biotechnol 75, 87–93 (2007).

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  • β-fructofuranosidase
  • Fructosyltransferase
  • Fructooligosaccharides
  • Aspergillus