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Rare carotenoids, (3R)-saproxanthin and (3R,2′S)-myxol, isolated from novel marine bacteria (Flavobacteriaceae) and their antioxidative activities

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Abstract

We isolated three orange or yellow pigment-producing marine bacteria, strains 04OKA-13-27 (MBIC08261), 04OKA-17-12 (MBIC08260), and YM6-073 (MBIC06409), off the coast of Okinawa Prefecture in Japan. These strains were classified as novel species of the family Flavobacteriaceae based on their 16S rRNA gene sequence. They were cultured, and the major carotenoids produced were purified by chromatographic methods. Their structures were determined by spectral data to be (3R)-saproxanthin (strain 04OKA-13-27), (3R,2′S)-myxol (strain YM6-073), and (3R,3′R)-zeaxanthin (strains YM6-073 and 04OKA-17-12). Saproxanthin and myxol, which are monocyclic carotenoids rarely found in nature, demonstrated significant antioxidative activities against lipid peroxidation in the rat brain homogenate model and a neuro-protective effect from l-glutamate toxicity.

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Acknowledgement

The hard coral used was identified by Hiroki Taniguchi of Akajima Marine Science Laboratory, to whom we are grateful. This work was supported in part by the Biotechnology and Medical Technology Development Department of the New Energy and Industrial Technology Development Organization of Japan (NEDO).

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Correspondence to Kazutoshi Shindo.

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Shindo, K., Kikuta, K., Suzuki, A. et al. Rare carotenoids, (3R)-saproxanthin and (3R,2′S)-myxol, isolated from novel marine bacteria (Flavobacteriaceae) and their antioxidative activities. Appl Microbiol Biotechnol 74, 1350–1357 (2007). https://doi.org/10.1007/s00253-006-0774-y

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Keywords

  • Carotenoid
  • Lycopene
  • Astaxanthin
  • Circular Dichroism Spectrum
  • Zeaxanthin