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Fe(III)-enhanced Azo Reduction by Shewanella decolorationis S12


Shewanella decolorationis S12 is capable of high rates of azo dye decolorization and dissimilatory Fe(III) reduction. Under anaerobic conditions, when Fe(III) and azo dye were copresent in S12 cultures, dissimilatory Fe(III) reduction and azo dye biodecolorization occurred simultaneously. Furthermore, the dye decolorization was enhanced by the presence of Fe(III). When 1 mM Fe(III) was added, the methyl red decolorizing efficiency was 72.1% after cultivation for 3 h, whereas the decolorizing efficiency was only 60.5% in Fe(III)-free medium. The decolorizing efficiencies increased as the concentration of Fe(III) was increased from 0 to 6 mM. Enzyme activities, which mediate the dye decolorization and Fe(III) reduction, were not affected by preadaption of cells to Fe(III) and azo dye nor by the addition of chloramphenicol. Both the Fe(III) reductase and the azo reductase were membrane associated. The respiratory electron transport chain inhibitors metyrapone, dicumarol, and stigmatellin showed significantly different effects on Fe(III) reduction than on azo dye decolorization.

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This research was supported by Chinese National Natural Science Foundations (3050009 and 30670020), Guangdong Provincial key Programs for Science and Technology Development (05100365), Guangdong Provincial Natural Science Foundation (No.015017), Guangdong Provincial Programs for Science and Technology Development (2006B36703001), and Guangzhou Programs for Science and Technology Development (2006Z3-E0461). In addition, Dr. Joy D. Van Nostrand was very kind to correct the language errors. Finally, we would like to thank the three anonymous reviewers for useful and constructive comments.

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Correspondence to Guoping Sun.

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Xu, M., Guo, J., Kong, X. et al. Fe(III)-enhanced Azo Reduction by Shewanella decolorationis S12. Appl Microbiol Biotechnol 74, 1342–1349 (2007). https://doi.org/10.1007/s00253-006-0773-z

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  • Dissimilatory Fe(III) reduction
  • Azo dye decolorization
  • Shewanella decolorationis S12
  • Enhancement