Applied Microbiology and Biotechnology

, Volume 72, Issue 4, pp 783–789 | Cite as

A leuC mutation leading to increased L-lysine production and rel-independent global expression changes in Corynebacterium glutamicum

  • Mikiro Hayashi
  • Hiroshi Mizoguchi
  • Junko Ohnishi
  • Satoshi Mitsuhashi
  • Yoshiyuki Yonetani
  • Shin-ichi Hashimoto
  • Masato IkedaEmail author
Genomics and Proteomics


We previously found by transcriptome analysis that global induction of amino acid biosynthetic genes occurs in a classically derived industrial l-lysine producer, Corynebacterium glutamicum B-6. Based on this stringent-like transcriptional profile in strain B-6, we analyzed the relevant mutations from among those identified in the genome of the strain, with special attention to the genes that are involved in amino acid biosynthesis and metabolism. Among these mutations, a Gly-456→Asp mutation in the 3-isopropylmalate dehydratase large subunit gene (leuC) was defined as a useful mutation. Introduction of the leuC mutation into a defined l-lysine producer, AHD-2 (hom59 and lysC311), by allelic replacement led to the phenotype of a partial requirement for l-leucine and approximately 14% increased l-lysine production. Transcriptome analysis revealed that many amino acid biosynthetic genes, including lysC-asd operon, were significantly upregulated in the leuC mutant in a rel-independent manner.


Cornynebacterium glutamicum l-lysine Fermentation DNA array 



The authors thank Drs. S. Teshiba and A. Ozaki for encouraging support of our work, and also T. Abe, Y. Adachi, S. Nakagawa, S. Koizumi, and M. Yagasaki for useful discussions, and A. Yamane and K. Ishimaru for their excellent technical assistance.


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Mikiro Hayashi
    • 1
  • Hiroshi Mizoguchi
    • 1
  • Junko Ohnishi
    • 1
  • Satoshi Mitsuhashi
    • 1
  • Yoshiyuki Yonetani
    • 1
  • Shin-ichi Hashimoto
    • 2
  • Masato Ikeda
    • 3
    Email author
  1. 1.BioFrontier LaboratoriesKyowa Hakko Kogyo Co., Ltd.TokyoJapan
  2. 2.Technical Research LaboratoriesKyowa Hakko Kogyo Co., Ltd.YamaguchiJapan
  3. 3.Department of Bioscience and Biotechnology,Faculty of AgricultureShinshu UniversityNaganoJapan

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