The new marine Halomonas sp. strain GWS-BW-H8hM (DSM 17996) was found to produce 3-(4′-hydroxyphenyl)-4-phenylpyrrole-2,5-dicarboxylic acid (HPPD-1) and 3,4-bis(4′-hydroxy- phenyl)pyrrole-2,5-dicarboxylic acid (HPPD-2). In initial cultivations using marine broth, only low contents of these compounds have been isolated. Improving the conditions and growing the strain on artificial seawater supplemented with tryptone 10 g l−1, yeast extract 5 g l−1, l-tyrosine 0.6 g l−1, glycine 1 g l−1, and glucose 6 g l-1, the growth-associated HPPD-1 and HPPD-2 production of a 40-l batch cultivation reached the amounts of 47 mg l−1 and 116 mg l−1, respectively, after 65 h. Both compounds showed potent anti-tumor-promoting activities.
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The authors would like to thank very much B. Körner and M. Karaman for experimental help. This work was supported by the Volkswagen Foundation within the Lower Saxonian priority program ‘Marine Biotechnology’.
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Wang, L., Groβe, T., Stevens, H. et al. Bioactive hydroxyphenylpyrrole-dicarboxylic acids from a new marine Halomonas sp.: production and structure elucidation. Appl Microbiol Biotechnol 72, 816–822 (2006). https://doi.org/10.1007/s00253-006-0370-1
- Dicarboxylic Acid
- Artificial Seawater
- Marine Broth
- Iron Citrate