Applied Microbiology and Biotechnology

, Volume 72, Issue 4, pp 732–737 | Cite as

Therapeutic effect of llama derived VHH fragments against Streptococcus mutans on the development of dental caries

  • Carina Krüger
  • Anna Hultberg
  • Harold Marcotte
  • Pim Hermans
  • Sandra Bezemer
  • Leon G. J. Frenken
  • Lennart HammarströmEmail author
Biotechnologically Relevant Enzymes and Proteins


Streptococcus mutans is the main cause of dental caries. We evaluated the therapeutic effect of variable regions of a llama heavy chain antibody fragments directed against S. mutans named S36-VHH (S for Streptococcus) alone or fused with glucose oxidase (GOx) from Aspergillus niger. Western blot analysis and ELISA revealed binding of the S36-VHH to the streptococcal antigen I/II adhesin molecule of S. mutans serotype C. In a rat-desalivated caries model, daily administration of S36-VHH significantly reduced the development of smooth surface caries. No additional therapeutic effect of GOx was observed. Our results suggest that llama VHH antibodies may be a potential benefit as prophylaxis against dental caries.


Dental Caries Streptococcus Mutans Heavy Chain Antibody Streptococcal Antigen Talon Metal Affinity Resin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the Foundation for Preventive Caries Research (Patentmedelsfonden) and the Swedish Research Council.


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Carina Krüger
    • 1
  • Anna Hultberg
    • 1
  • Harold Marcotte
    • 1
  • Pim Hermans
    • 2
  • Sandra Bezemer
    • 2
  • Leon G. J. Frenken
    • 3
  • Lennart Hammarström
    • 1
    Email author
  1. 1.Division of Clinical ImmunologyKarolinska Institutet at Karolinska University HospitalStockholmSweden
  2. 2.Biotechnology Application Centre (BAC) BVNaardenThe Netherlands
  3. 3.Unilever Foods and Health Research InstituteVlaardingenThe Netherlands

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