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Monitoring Arthrobacter protophormiae RKJ100 in a ‘tag and chase’ method during p-nitrophenol bio-remediation in soil microcosms

Abstract

Monitoring of micro-organisms released deliberately into the environment is essential to assess their movement during the bio-remediation process. During the last few years, DNA-based genetic methods have emerged as the preferred method for such monitoring; however, their use is restricted in cases where organisms used for bio-remediation are not well characterized or where the public domain databases do not provide sufficient information regarding their sequence. For monitoring of such micro-organisms, alternate approaches have to be undertaken. In this study, we have specifically monitored a p-nitrophenol (PNP)-degrading organism, Arthrobacter protophormiae RKJ100, using molecular methods during PNP degradation in soil microcosm. Cells were tagged with a transposon-based foreign DNA sequence prior to their introduction into PNP-contaminated microcosms. Later, this artificially introduced DNA sequence was PCR-amplified to distinguish the bio-augmented organism from the indigenous microflora during PNP bio-remediation.

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Acknowledgements

We are indebted to Debarati Paul for helpful discussions, to Aparajita Basu for her excellent technical assistance and to Dr. Karl-Heinz Gartemann for kindly providing the transposon delivery vector. This work, in part, was supported by the Indo Swiss Collaboration in Biotechnology (ISCB) and Department of Biotechnology (DBT). This is IMTECH Communication No. 36/2004.

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Correspondence to Rakesh K. Jain.

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Pandey, G., Pandey, J. & Jain, R.K. Monitoring Arthrobacter protophormiae RKJ100 in a ‘tag and chase’ method during p-nitrophenol bio-remediation in soil microcosms. Appl Microbiol Biotechnol 70, 757–760 (2006). https://doi.org/10.1007/s00253-005-0134-3

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Keywords

  • Soil Microcosm
  • Microbial Potential
  • Soil Microcosm Experiment
  • Strain RKJ100
  • Arthrobacter Protophormiae