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Enhancement of a pentacyclic tyrosine kinase inhibitor production in Cladosporium cf. cladosporioides by Cladosporol

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The binaphthyl derivative cladosporol 3 was supplied from 60 to 200 mg l−1 to shaken cultures of Cladosporium cf. cladosporioides. Compared to blank, fungal biomass was not affected by adding cladosporol till 100 mg l−1: it rather increased at higher ratios between 150 and 200 mg l−1. The production of the major pentacyclic metabolite 1, a cytokine production and tyrosine kinase inhibitor, was enhanced tenfold when cladosporol was supplied at the highest ratio (200 mg l−1) to shaken growing cultures of the fungus. The bioconversion of cladosporol to cladosporol D through reductive cleavage of the epoxide group was also observed. Interest in this kind of metabolites lies in their potential activity vs DNA topoisomerase I.

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This work was in part supported by a Grant of MIUR-FIRB (Italy). Thanks are due to Prof. Wrigley and his collaborators for the C. cladosporioides culture and helpful discussion on results.

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Correspondence to Gemma Assante.

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Assante, G., Bava, A. & Nasini, G. Enhancement of a pentacyclic tyrosine kinase inhibitor production in Cladosporium cf. cladosporioides by Cladosporol. Appl Microbiol Biotechnol 69, 718–721 (2006). https://doi.org/10.1007/s00253-005-0054-2

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  • Biotransformation
  • Cladosporium
  • Reductive Cleavage
  • Binaphthyl
  • Specific Cell Activity