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European Biophysics Journal

, Volume 48, Issue 7, pp 659–671 | Cite as

Impact of semen-derived amyloid (SEVI) on sperm viability and motility: its implication in male reproductive fitness

  • Vijay Kumar
  • Pradeep G. Kumar
  • Jay Kant YadavEmail author
Original Article

Abstract

Human semen contains a large number of macromolecules, including proteins/enzymes and carbohydrates, regulating and protecting sperm cells. Proteomic analysis of human seminal fluid led to the discovery of semen amyloids derived from short peptide fragments of the proteins prostatic acid phosphatase (PAP) and semenogelin (SG) which are known to play a crucial role in enhancing HIV infection. However, the relevance of their existence in human semen and role in maintaining sperm behavior remains unclear. Distinct physiological, biochemical, and biophysical attributes might cause these amyloids to influence sperm behavior positively or negatively, affecting fertilization or other reproductive processes. We assessed the direct effect of amyloids derived from a PAP248–286 fragment, on sperm motility and viability, which are crucial parameters for assessment of sperm quality in semen. Co-incubation of human sperm with PAP248–286 amyloids at normal physiological concentrations formed in buffer led to significant reduction in sperm viability, though approximately a 10× higher concentration was needed to show a similar effect with amyloid formed in seminal fluid. Both forms of PAP248–286 amyloid also had a significant impact on sperm motility at physiological levels, in agreement with a previous report. Our study suggests that PAP248–286 amyloids can directly influence sperm motility and viability in a concentration-dependent manner. We hypothesise that the direct toxic effect of PAP248–286 amyloid is normally mitigated by other seminal fluid ingredients, but that in pathological conditions, where PAP248–286 concentrations are elevated and it plays a role in determining sperm health and viability, with relevance for male fertility as well as sterility.

Keywords

Semen-derived amyloids PAP248–286 SEVI Male sterility 

Abbreviations

PAP

Prostatic acid phosphatase

SG

Semenogelin

SEVI

Semen-derived enhancer of viral infection

SP

Seminal plasma

DPBS

Dulbecco’s phosphate buffer saline

RPM

Revolutions per minutes

CR

Congo red

TEM

Transmission electron microscope

SMI

Sperm motility index

PI

Propidium iodide

FACS

Fluorescence-activated cell sorting

Notes

Acknowledgements

We are thankful to the technical staff of central instrument facility at RGCB, and KJK hospital Thiruvananthapuram, Kerala, India for their unconditional support. Mr. Vijay Kumar is thankful to the lab members of molecular reproduction at RGCB for their help during this project. Mr. Vijay Kumar acknowledges University Grants Commission, India and Social Justice and Empowerment Department, Govt. of India for RGN fellowship.

Funding

This study was partially supported by Department of Science and Technology, Government of India (Grant number SB/YS/LS-130/2013).

Compliance with ethical standards

Conflict of interest

The authors do not have any conflict of interest to declare.

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Copyright information

© European Biophysical Societies' Association 2019

Authors and Affiliations

  1. 1.Department of BiotechnologyCentral University of RajasthanAjmerIndia
  2. 2.Molecular Reproduction DivisionRajiv Gandhi Centre for BiotechnologyThiruvananthapuramIndia

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