57Fe enrichment in mice for β-thalassaemia studies via Mössbauer spectroscopy of blood samples
In this work, wild-type and heterozygous β-thalassaemic mice were enriched with 57Fe via gastrointestinal absorption to characterize in greater detail the iron complexes then identifiable via Mössbauer spectroscopy. The 57Fe enrichment method was validated and Mössbauer spectra were obtained at 80 K from blood samples from wild-type and β-thalassaemic mice at 1, 3, 6, and 9 months of age. As expected, the haemoglobin levels of the thalassaemic mice were lower than from normal mice, indicating anaemia. Furthermore, significant amounts of ferritin-like iron were observed in the thalassaemic mice samples, which decreased with mouse age, reflecting the pattern of reticulocyte count reduction reported in the literature.
KeywordsMössbauer spectroscopy β-Thalassaemia Mice 57Fe enrichment Ferritin Blood
We gratefully acknowledge the personnel of the Transgenic Mouse Facility of the Cyprus Institute of Neurology and Genetics for the planning of the mouse breeding and mouse feed preparation guidance.
Compliance with ethical standards
All applicable international, national and/or institutional guidelines for the care and use of animals were followed.
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