Thermodynamics of protein folding: methodology, data analysis and interpretation of data

  • Seyedeh Akram Shirdel
  • Khosrow KhalifehEmail author
Technical Primer


Thermodynamics of protein folding refers to the stability measurements where structural changes of a given protein in the presence of a denaturing agent are monitored by spectroscopic or calorimetric techniques. In macroscopic point of view, protein stability represents the ratio of the population of its unfolded state to that of folded one in equilibrium condition, while in microscopic point of view, the stability is actually a net value from a combination of favorable and unfavorable contributions that affect the structural integrity of a protein molecule. In this manuscript, the principles and methodological aspects of thermodynamic studies and methods of data analysis as well as interpretation of the results are presented.

Graphical abstract


Thermodynamics Folding Stability Equilibrium Protein Data analysis 



The authors confirm that there are no known conflicts of interest associated with this publication. There has been no significant financial support for this work.


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© European Biophysical Societies' Association 2019

Authors and Affiliations

  1. 1.Department of Biochemistry, Faculty of Biological SciencesTarbiat Modares UniversityTehranIran
  2. 2.Department of Biology, Faculty of SciencesUniversity of ZanjanZanjanIran

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