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European Biophysics Journal

, Volume 48, Issue 3, pp 231–248 | Cite as

Algal cell response to laboratory-induced cadmium stress: a multimethod approach

  • Nadica Ivošević DeNardisEmail author
  • Jadranka Pečar Ilić
  • Ivica Ružić
  • Nives Novosel
  • Tea Mišić Radić
  • Andreas Weber
  • Damir Kasum
  • Zuzana Pavlinska
  • Ria Katalin Balogh
  • Bálint Hajdu
  • Alžbeta Marček Chorvátová
  • Béla Gyurcsik
Original Article

Abstract

We examined the response of algal cells to laboratory-induced cadmium stress in terms of physiological activity, autonomous features (motility and fluorescence), adhesion dynamics, nanomechanical properties, and protein expression by employing a multimethod approach. We develop a methodology based on the generalized mathematical model to predict free cadmium concentrations in culture. We used algal cells of Dunaliella tertiolecta, which are widespread in marine and freshwater systems, as a model organism. Cell adaptation to cadmium stress is manifested through cell shape deterioration, slower motility, and an increase of physiological activity. No significant change in growth dynamics showed how cells adapt to stress by increasing active surface area against toxic cadmium in the culture. It was accompanied by an increase in green fluorescence (most likely associated with cadmium vesicular transport and/or beta-carotene production), while no change was observed in the red endogenous fluorescence (associated with chlorophyll). To maintain the same rate of chlorophyll emission, the cell adaptation response was manifested through increased expression of the identified chlorophyll-binding protein(s) that are important for photosynthesis. Since production of these proteins represents cell defence mechanisms, they may also signal the presence of toxic metal in seawater. Protein expression affects the cell surface properties and, therefore, the dynamics of the adhesion process. Cells behave stiffer under stress with cadmium, and thus, the initial attachment and deformation are slower. Physicochemical and structural characterizations of algal cell surfaces are of key importance to interpret, rationalize, and predict the behaviour and fate of the cell under stress in vivo.

Keywords

Adhesion kinetics Autofluorescence Cadmium bioavailability Cell stress adaptation Nanomechanics Protein expression 

Notes

Acknowledgements

This work was conducted and supported by project Algal cell biophysical properties as markers for environmental stress in aquatic systems (ID 21720055) funded through the International Visegrad Fund. AMH acknowledges support from the Integrated Initiative of European Laser Infrastructures LASERLAB-EUROPE IV (H2020 grant agreement no. 654148). AW acknowledges funding from the Austrian Science Fund (project number P29562N28). We would like to thank (i) Tarzan Legović for discussing cell motility analysis with us, (ii) Jagoba Iturri and José Luis Toca-Herrera for discussing cell nanomechanics with AW and (iii) project partner Josef Sepitka for his participation and interest in this work. The authors acknowledge networking effort within COST Action CA15126 ARBRE MOBIEU.

Supplementary material

249_2019_1347_MOESM1_ESM.pdf (386 kb)
Supplementary material 1 (PDF 386 kb)

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Copyright information

© European Biophysical Societies' Association 2019

Authors and Affiliations

  • Nadica Ivošević DeNardis
    • 1
    Email author
  • Jadranka Pečar Ilić
    • 1
  • Ivica Ružić
    • 1
  • Nives Novosel
    • 2
  • Tea Mišić Radić
    • 1
  • Andreas Weber
    • 3
  • Damir Kasum
    • 1
  • Zuzana Pavlinska
    • 4
    • 5
  • Ria Katalin Balogh
    • 6
  • Bálint Hajdu
    • 6
  • Alžbeta Marček Chorvátová
    • 4
    • 5
  • Béla Gyurcsik
    • 6
  1. 1.Division for Marine and Environmental ResearchRuđer Bošković InstituteZagrebCroatia
  2. 2.Department of Biology, Faculty of ScienceUniversity of ZagrebZagrebCroatia
  3. 3.Department of Nanobiotechnology, Institute for BiophysicsUniversity of Natural Resources and Life SciencesViennaAustria
  4. 4.Department of Biophysics, Faculty of Natural SciencesUniversity of Ss. Cyril and MethodiusTrnavaSlovakia
  5. 5.Department of BiophotonicsInternational Laser CentreBratislavaSlovakia
  6. 6.Department of Inorganic and Analytical ChemistryUniversity of SzegedSzegedHungary

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