Effects of Quorum Quenching on Biofilm Metacommunity in a Membrane Bioreactor
- 167 Downloads
Quorum quenching (QQ) has received attention for the control of biofilms, e.g., biofilms that cause biofouling in membrane bioreactors (MBRs). Despite the efficacy of QQ on biofouling, it is elusive how QQ influences biofilm formation on membranes. A pilot-scale QQ-MBR and non-QQ-MBR were identically operated for 4 days and 8 days to destructively sample the membranes. QQ prolonged the membrane filterability by 43% with no harmful influence on MBR performance. qPCR showed no effect of QQ on microbial density during either of these time periods. Community comparisons revealed that QQ influenced the bacterial and fungal community structures, and the fungal structure corresponded with the bacterial structure. Metacommunity and spatial analyses showed that QQ induced structural variation rather than compositional variation of bacteria and fungi. Moreover, QQ considerably enhanced the bacterial dispersal across membrane during the early development. As the dispersal enhancement by QQ counteracted the ecological drift, it eliminated the distance–decay relationship, reflecting a neutral theory archetype of metacommunity. Network analyses showed that QQ substantially reduced the amount and magnitude of interactions, e.g., competition and cooperation, for bacteria and fungi, and weakened their network structures, irrespective of time. Additionally, QQ suppressed the growth of specific microbial species (e.g., Acinetobacter), abundant and widespread at the early stage. These findings suggest that QQ influenced the community dynamics at the regional and local levels, correspondingly the ecological selection and dispersal processes, during the biofilm development.
KeywordsMetacommunity Biofilm Quorum quenching Biofouling Membrane bioreactor
This study was supported by the basic science research program through the National Research Foundation of Korea funded by the Ministry of Education (2018R1D1A1B07048872).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 4.Lee S, Park S-K, Kwon H, Lee SH, Lee K, Nahm CH, Jo SJ, Oh H-S, Park P-K, Choo K-H, Lee C-H, Yi T (2016) Crossing the border between laboratory and field: bacterial quorum quenching for anti-biofouling strategy in an MBR. Environ Sci Technol 50:1788–1795. https://doi.org/10.1021/acs.est.5b04795 CrossRefPubMedGoogle Scholar
- 8.Oh H-S, Yeon K-M, Yang C-S, Kim S-R, Lee C-H, Park SY, Han JY, Lee J-K (2012) Control of membrane biofouling in MBR for wastewater treatment by quorum quenching bacteria encapsulated in microporous membrane. Environ Sci Technol 46:4877–4884. https://doi.org/10.1021/es204312u CrossRefPubMedGoogle Scholar
- 12.Leibold MA, Holyoak M, Mouquet N, Amarasekare P, Chase JM, Hoopes MF, Holt RD, Shurin JB, Law R, Tilman D, Loreau M, Gonzalez A (2004) The metacommunity concept: a framework for multi-scale community ecology. Ecol Lett 7:601–613. https://doi.org/10.1111/j.1461-0248.2004.00608.x CrossRefGoogle Scholar
- 13.Leibold MA, Chase JM (2018) Metacommunity ecology. Princeton University PressGoogle Scholar
- 15.Tan CH, Koh KS, Xie C, Tay M, Zhou Y, Williams R, Ng WJ, Rice SA, Kjelleberg S (2014) The role of quorum sensing signalling in EPS production and the assembly of a sludge community into aerobic granules. ISME J 8:1186–1197. https://doi.org/10.1038/ismej.2013.240 CrossRefPubMedPubMedCentralGoogle Scholar
- 22.Deveau A, Bonito G, Uehling J, Paoletti M, Becker M, Bindschedler S, Hacquard S, Hervé V, Labbé J, Lastovetsky OA, Mieszkin S, Millet LJ, Vajna B, Junier P, Bonfante P, Krom BP, Olsson S, van Elsas JD, Wick LY (2018) Bacterial–fungal interactions: ecology, mechanisms and challenges. FEMS Microbiol Rev 42:335–352. https://doi.org/10.1093/femsre/fuy008 CrossRefPubMedGoogle Scholar
- 29.Nemergut DR, Schmidt SK, Fukami T, O’Neill SP, Bilinski TM, Stanish LF, Knelman JE, Darcy JL, Lynch RC, Wickey P, Ferrenberg S (2013) Patterns and processes of microbial community assembly. Microbiol Mol Biol Rev 77:342–356. https://doi.org/10.1128/mmbr.00051-12 CrossRefPubMedPubMedCentralGoogle Scholar