Advertisement

Microbial Ecology

, Volume 78, Issue 4, pp 792–803 | Cite as

Influences of Iron Compounds on Microbial Diversity and Improvements in Organic C, N, and P Removal Performances in Constructed Wetlands

  • Zhimiao Zhao
  • Xiao Zhang
  • Mengqi Cheng
  • Xinshan SongEmail author
  • Yinjiang Zhang
  • Xiangmei Zhong
Microbiology of Aquatic Systems
  • 148 Downloads

Abstract

The effects of various combinations of iron compounds on the contaminant removal performance in constructed wetlands (CWs) were explored under various initial iron concentrations, contaminant concentrations, different hydraulic retention time (HRT), and different temperatures. The Combo 6 (nanoscale zero-valent iron combined with Fe3+) in CW treatments showed the highest pollutant removal performance under the conditions of C2 initial iron dosage concentration (total iron 0.2 mM) and I2 initial contaminant concentration (COD:TN:TP = 60 mg/L:60 mg/L:1 mg/L) in influent after 72-h HRT. These results were directly verified by two different microbial tests (Biolog test and high-throughput pyrosequencing) and microbial community analysis (principal component analysis of community-level physiological profile, biodiversity index, cluster tree, relative abundance at order of taxonomy level). Specific bacteria related to significant improvements in contaminant removal were domesticated by various combinations of iron compounds. Iron dosage was advised as a green, new, and effective option for wastewater treatment.

Graphical Abstract

.

Keywords

Combinations of iron compounds Multiple contaminants Oxidation-reduction Microbial community optimization 

Supplementary material

248_2019_1379_MOESM1_ESM.docx (2 mb)
ESM 1 (DOCX 2000 kb)

References

  1. 1.
    Xu P, Zeng GM, Huang DL, Feng CL, Hu S, Zhao MH, Lai C, Wei Z, Huang C, Xie GX, Liu ZF (2012) Use of iron oxide nanomaterials in wastewater treatment: a review. Sci Total Environ 424:1–10.  https://doi.org/10.1016/j.scitotenv.2012.02.023 CrossRefPubMedGoogle Scholar
  2. 2.
    Kumar P, Prot T, Korving L, Keesman KJ, Dugulan L, Van Loosdrecht MCM, Witkamp GJ (2017) Effect of pore size distribution on iron oxide coated granular activated carbons for phosphate adsorption - importance of mesopores. Chem Eng J 326:231–239.  https://doi.org/10.1016/j.cej.2017.05.147 CrossRefGoogle Scholar
  3. 3.
    Ma XC, Zhou WG, Fu ZQ, Cheng YL, Min M, Liu YH, Zhang YK, Chen P, Ruan R (2014) Effect of wastewater-borne bacteria on algal growth and nutrients removal in wastewater-based algae cultivation system. Bioresour Technol 167:8–13.  https://doi.org/10.1016/j.biortech.2014.05.087 CrossRefPubMedGoogle Scholar
  4. 4.
    Skoog A, Arias-Esquivel VA (2009) The effect of induced anoxia and re-oxygenation on benthic fluxes of organic carbon, phosphate, iron, and manganese. Sci Total Environ 407:6085–6092.  https://doi.org/10.1016/j.scitotenv.2009.08.030 CrossRefPubMedGoogle Scholar
  5. 5.
    Zhou LJ, Zhuang WQ, Wang X, Yu K, Yang SF, Xia SQ (2017) Potential effects of loading nano zero valent iron discharged on membrane fouling in an anoxic/oxic membrane bioreactor. Water Res 111:140–146.  https://doi.org/10.1016/j.watres.2017.01.007 CrossRefPubMedGoogle Scholar
  6. 6.
    Choi H, Al-Abed SR, Agarwal S, Dionysiou D (2008) Synthesis of reactive nano-Fe/Pd bimetallic system-impregnated activated carbon for the simultaneous adsorption and dechlorination of PCBs. Chem Mater 20(11):3649–3655.  https://doi.org/10.1021/cm8003613 CrossRefGoogle Scholar
  7. 7.
    O’Carroll D, Sleep B, Krol M, Boparai H, Kocur C (2013) Nanoscale zero valent iron and bimetallic particles for contaminated site remediation. Adv Water Resour 51:104–122.  https://doi.org/10.1016/j.advwatres.2012.02.005 CrossRefGoogle Scholar
  8. 8.
    Kumar MA, Choe JK, Lee WJ, Yoon SH (2017) Synthesis of benzaldoxime from benzaldehyde using nanoscale zero-valent iron and dissolved nitrate or nitrite. Environ Nanotechnol Monit Manage 8:97–102.  https://doi.org/10.1016/j.enmm.2017.06.003 CrossRefGoogle Scholar
  9. 9.
    Lu Q, Jeen SW, Gui L, Gillham RW (2017) Nitrate reduction and its effects on trichloroethylene degradation by granular iron. Water Res 112:48–57.  https://doi.org/10.1016/j.watres.2017.01.031 CrossRefPubMedGoogle Scholar
  10. 10.
    Jordan TE, Cornwell JC, Boynton WR, Anderson JT (2008) Changes in phosphorus biogeo-chemistry along an estuarine salinity gradient: the iron conveyer belt. Limnol Oceanogr 53:172–184.  https://doi.org/10.4319/lo.2008.53.1.0172 CrossRefGoogle Scholar
  11. 11.
    Song XS, Wang SY, Wang YH, Zhao ZM, Yan DH (2016) Addition of Fe2+ increase nitrate removal in vertical subsurface flow constructed wetlands. Ecol Eng 91:487–494.  https://doi.org/10.1016/j.ecoleng.2016.03.013 CrossRefGoogle Scholar
  12. 12.
    Rodriguez-Narvaez OM, Peralta-Hernandez JM, Goonetilleke A, Bandala ER (2017) Treatment technologies for emerging contaminants in water: a review. Chem Eng J 323:261–380.  https://doi.org/10.1016/j.cej.2017.04.106 CrossRefGoogle Scholar
  13. 13.
    Zhao ZM, Song XS, Wang W, Xiao YP, Gong ZJ, Wang YH, Zhao YF, Chen Y, Mei MY (2016) Influences of iron and calcium carbonate on wastewater treatment performances of algae-based reactors. Bioresour Technol 216:1–11.  https://doi.org/10.1016/j.biortech.2016.05.043 CrossRefPubMedGoogle Scholar
  14. 14.
    Vymazal J (2007) Removal of nutrients in various types of constructed wetlands. Sci Total Environ 380:48–65.  https://doi.org/10.1016/j.scitotenv.2006.09.014 CrossRefPubMedGoogle Scholar
  15. 15.
    Vymazal J (2010) Constructed wetlands for wastewater treatment: five decades of experience. Environ Sci Technol 45(1):61–69.  https://doi.org/10.1021/es101403q CrossRefPubMedGoogle Scholar
  16. 16.
    Zhao ZM, Song XS, Zhao YF, Xiao YP, Wang YH, Wang JF, Yan DH (2017) Effects of iron and calcium carbonate on the variation and cycling of carbon source in integrated wastewater treatments. Bioresour Technol 225:262–271.  https://doi.org/10.1016/j.biortech.2016.11.074 CrossRefGoogle Scholar
  17. 17.
    Zhao ZM, Song XS, Xiao YP, Zhao YF, Gong ZJ, Lin FD, Ding Y, Wang W, Qin TL (2016) Influences of seasons, N/P ratios and chemical compounds on phosphorus removal performance in algal pond combined with constructed wetlands. Sci Total Environ 573:906–914.  https://doi.org/10.1016/j.scitotenv.2016.08.148 CrossRefGoogle Scholar
  18. 18.
    Zhao ZM, Song XS, Zhang YJ, Zhao YF, Wang BD, Wang YH (2017) Effects of iron and calcium carbonate on contaminant removal efficiencies and microbial communities in integrated wastewater treatment systems. Chemosphere 189:10–20.  https://doi.org/10.1016/j.chemosphere.2017.09.020 CrossRefPubMedGoogle Scholar
  19. 19.
    Choi KH, Dobbs FC (1999) Comparison of two kinds of Biolog microplates (GN and ECO) in their ability to distinguish among aquatic microbial communities. J Microbiol Methods 36:203–213.  https://doi.org/10.1016/S0167-7012(99)00034-2 CrossRefPubMedGoogle Scholar
  20. 20.
    Pan F, Xu AH, Xia DS, Yu Y, Chen G, Meyer M, Zhao DY, Huang CH, Wu QW, Fu J (2015) Effects of octahedral molecular sieve on treatment performance, microbial metabolism, and microbial community in expanded granular sludge bed reactor. Water Res 87:127–136.  https://doi.org/10.1016/j.watres.2015.09.022 CrossRefPubMedGoogle Scholar
  21. 21.
    Allen B, Kon M, Bar-Yam Y (2009) A new phylogenetic diversity measure generalizing the Shannon index and its application to phyllostomid bats. Am Nat 174(2):236–243.  https://doi.org/10.1086/600101 CrossRefPubMedGoogle Scholar
  22. 22.
    Jiang L, Han GM, Lan Y, Liu SN, Gao JP, Yang X, Meng J, Chen WF (2017) Corn cob biochar increases soil culturable bacterial abundance without enhancing their capacities in utilizing carbon sources in Biolog Eco-plates. J Integr Agric 16(3):713–724.  https://doi.org/10.1016/S2095-3119(16)61338-2 CrossRefGoogle Scholar
  23. 23.
    Rutgers M, Wouterse M, Drost SM, Breure AM, Mulder C, Stone D, Creamer RE, Winding A, Bloem J (2016) Monitoring soil bacteria with community-level physiological profiles using Biolog TM ECO-plates in the Netherlands and Europe. Appl Soil Ecol 97:23–35.  https://doi.org/10.1016/j.apsoil.2015.06.007 CrossRefGoogle Scholar
  24. 24.
    Chang J, Wu SQ, Dai Y, Liang W, Wu ZB (2012) Treatment efficiency of integrated vertical-flow constructed wetland plots for domestic wastewater. Ecol Eng 44:152–159.  https://doi.org/10.1016/j.ecoleng.2012.03.019 CrossRefGoogle Scholar
  25. 25.
    Akratos CS, Tsihrintzis VA (2007) Effect of temperature, HRT, vegetation and porous media on removal efficiency of pilot-scale horizontal subsurface flow constructed wetlands. Ecol Eng 29(2):173–191.  https://doi.org/10.1016/j.ecoleng.2006.06.013 CrossRefGoogle Scholar
  26. 26.
    Mateus DMR, Vaz MN, Pinho HJO (2012) Fragmented limestone wastes as a constructed wetland substrate for phosphorus removal. Ecol Eng 41:65–69.  https://doi.org/10.1016/j.ecoleng.2012.01.014 CrossRefGoogle Scholar
  27. 27.
    Ding Y, Song XS, Wang YH, Yan DH (2013) Effect of supplying a carbon extracting solution on denitrification in horizontal subsurface flow constructed wetlands. Korean J Chem Eng 30(2):379–384.  https://doi.org/10.1007/s11814-012-0139-4 CrossRefGoogle Scholar
  28. 28.
    Moussavi G, Jafari SJ, Yaghmaeian K (2015) Enhanced biological denitrification in the cyclic rotating bed reactor with catechol as carbon source. Bioresour Technol 189:266–272.  https://doi.org/10.1016/j.biortech.2015.04.019 CrossRefPubMedGoogle Scholar
  29. 29.
    Stefanakis AI, Tsihrintzis VA (2012) Effects of loading, resting period, temperature, porous media, vegetation and aeration on performance of pilot-scale vertical flow constructed wetlands. Chem Eng J 181-182:416–430.  https://doi.org/10.1016/j.cej.2011.11.108 CrossRefGoogle Scholar
  30. 30.
    Picardal F (2012) Abiotic and microbial interactions during anaerobic transformations of Fe (II) and NOx-. Front Microbiol 3:1–7.  https://doi.org/10.3389/fmicb.2012.00112 CrossRefGoogle Scholar
  31. 31.
    Zhao ZM, Song XS, Wang YH, Wang DY, Wang SY, He Y, Ding Y, Wang W, Yan DH, Wang JF (2016c) Effects of algal ponds on vertical flow constructed wetlands under different sewage application techniques. Ecol Eng 93:120–128.  https://doi.org/10.1016/j.ecoleng.2016.05.033 CrossRefGoogle Scholar
  32. 32.
    Shrestha J, Rich J, Ehrenfeld JG, Jaffe PR (2009) Oxidation of ammonium to nitrite under iron-reducing conditions in wetland soils: laboratory, field demonstrations, and push-pull rate determination. Soil Sci 174:156–164.  https://doi.org/10.1097/SS.0b013e3181988fbf CrossRefGoogle Scholar
  33. 33.
    Straub KL, Benz M, Schink B, Widdel F (1996) Anaerobic: nitrate-dependent microbial oxidation of ferrous iron. Appl Environ Microbiol 62:1458–1460.  https://doi.org/10.1016/0921-8777(95)00057-7 CrossRefPubMedPubMedCentralGoogle Scholar
  34. 34.
    Rozan TF, Taillefert M, Trouwborst RE, Glazer BT, Ma S, Herszage J, Lexia MV, Kent SP, George WL (2002) Iron-sulfur-phosphorus cycling in the sediments of a shallow coastal bay: implications for sediment nutrient release and benthic microalga blooms. Limnol Oceanogr 47:1346–1354.  https://doi.org/10.4319/lo.2002.47.5.1346 CrossRefGoogle Scholar
  35. 35.
    Bruch I, Fritsche J, Bänninger D, Alewell U, Sendelov M, Hürlimann H, Hasselbach R, Alewell C (2011) Improving the treatment efficiency of constructed wetlands with zeolite-containing filter sands. Bioresour Technol 102:937–941.  https://doi.org/10.1016/j.biortech.2010.09.0 CrossRefPubMedGoogle Scholar
  36. 36.
    Bongoua-Devisme AJ, Mustin C, Berthelin J (2012) Responses of iron-reducing bacteria to salinity and organic matter amendment in paddy soils of Thailand. Pedosphere 22:375–393.  https://doi.org/10.1016/S1002-0160(12)60024-1 CrossRefGoogle Scholar
  37. 37.
    Li XQ, Zhang WX (2006) Iron nanoparticles: the core-shell structure and unique properties for Ni (II) sequestration. Langmuir 22:4638–4642.  https://doi.org/10.1021/la060057k CrossRefPubMedGoogle Scholar
  38. 38.
    Li L, Stanforth R (2000) Distinguishing adsorption and surface precipitation of phosphate on goethite (a-FeOOH). J Colloid Interface Sci 230:12–21.  https://doi.org/10.1006/jcis.2000.7072 CrossRefPubMedGoogle Scholar
  39. 39.
    Asnicar F, Weingart G, Tickle TL, Huttenhower C, Segata N (2015) Compact graphical representation of phylogenetic data and metadata with GraPhlAn. Peer J 3:e1029.  https://doi.org/10.7717/peerj.1029 CrossRefPubMedGoogle Scholar
  40. 40.
    Vandewalle JL, Goetz GW, Huse SM, Morrison HG, Sogin ML, Hoffmann RG, Yan K, Mclellan SL (2012) Acinetobacter, Aeromonas and Trichococcus populations dominate the microbial community within urban sewer infrastructure. Environ Microbiol 14(9):2538–2552.  https://doi.org/10.1111/j.1462-2920.2012.02757.x CrossRefPubMedPubMedCentralGoogle Scholar
  41. 41.
    Mohanakrishnan J, Gutierrez O, Sharma KR, Guisasola A, Werner U, Meyer RL, Keller J, Yuan Z (2009) Impact of nitrate addition on biofilm properties and activities in rising main sewers. Water Res 43:4225–4237.  https://doi.org/10.1016/j.watres.2009.06.021 CrossRefPubMedGoogle Scholar
  42. 42.
    Kong X, Wang C, Ji M (2013) Analysis of microbial metabolic characteristics in mesophilic and thermophilic biofilters using Biolog plate technique. Chem Eng J 230:415–421.  https://doi.org/10.1016/j.cej.2013.06.073 CrossRefGoogle Scholar
  43. 43.
    Park SJ, Kim J, Lee JS, Rhee SK, Kim H (2014) Characterization of the fecal microbiome in different swine groups by high-through put sequencing. Anaerobe 28:157–162.  https://doi.org/10.1016/j.anaerobe.2014.06.002 CrossRefPubMedGoogle Scholar
  44. 44.
    Desai C, Parikh RY, Vaishnav T, Shouche YS, Madamwar D (2009) Tracking the influence of long-term chromium pollution on soil bacterial community structures by comparative analyses of 16S rRNA gene phylotypes. Res Microbiol 160:1–9.  https://doi.org/10.1016/j.resmic.2008.10.003 CrossRefPubMedGoogle Scholar
  45. 45.
    Sinninghe Damsté JS, Rijpstra WIC, Geenevasen JAJ, Strous M, Jetten MSM (2005) Structural identification of ladderane and other membrane lipids of planctomycetes capable of anaerobic ammonium oxidation (anammox). FEBS J 272:4270–4283.  https://doi.org/10.1111/j.1742-4658.2005.04842.x CrossRefPubMedGoogle Scholar
  46. 46.
    Dunfield PF, Yuryev A, Senin P, Angela VS, Matthew BS, Hou SB, Ly B, Saw JH, Zhou ZM, Ren Y, Wang JM, Mountain BW, Crowe MA, Weatherby TM, Bodelier PLE, Liesack W, Feng L, Wang L, Alam M (2007) Methane oxidation by an extremely acidophilic bacterium of the phylum Verrucomicrobia. Nature 450(7171):879–882.  https://doi.org/10.1038/nature06411 CrossRefPubMedGoogle Scholar
  47. 47.
    Yousuf B, Keshri J, Mishra A, Jha B (2012) Application of targeted metagenomics to explore abundance and diversity of CO2-fixing bacterial community using cbbL gene from the rhizosphere of Arachis hypogaea. Gene 506:18–24.  https://doi.org/10.1016/j.gene.2012.06.083 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Zhimiao Zhao
    • 1
    • 2
  • Xiao Zhang
    • 1
    • 2
  • Mengqi Cheng
    • 1
    • 2
  • Xinshan Song
    • 3
    Email author
  • Yinjiang Zhang
    • 1
    • 2
  • Xiangmei Zhong
    • 1
    • 2
  1. 1.College of Marine Ecology and EnvironmentShanghai Ocean UniversityShanghaiChina
  2. 2.Engineering Research Center for Water Environment Ecology in ShanghaiShanghaiChina
  3. 3.State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, College of Environmental Science and EngineeringDonghua UniversityShanghaiChina

Personalised recommendations