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Colonization and Competition Dynamics of Plant Growth-Promoting/Inhibiting Bacteria in the Phytosphere of the Duckweed Lemna minor

  • Hidehiro Ishizawa
  • Masashi Kuroda
  • Kanako Inoue
  • Daisuke Inoue
  • Masaaki Morikawa
  • Michihiko IkeEmail author
Plant Microbe Interactions
  • 72 Downloads

Abstract

Despite the considerable role of aquatic plant-associated bacteria in host plant growth and nutrient cycling in aquatic environments, the mode of their plant colonization has hardly been understood. This study examined the colonization and competition dynamics of a plant growth-promoting bacterium (PGPB) and two plant growth-inhibiting bacteria (PGIB) in the aquatic plant Lemna minor (common duckweed). When inoculated separately to L. minor, each bacterial strain quickly colonized at approximately 106 cells per milligram (plant fresh weight) and kept similar populations throughout the 7-day cultivation time. The results of two-membered co-inoculation assays revealed that the PGPB strain Aquitalea magnusonii H3 consistently competitively excluded the PGIB strain Acinetobacter ursingii M3, and strain H3 co-existed at almost 1:1 proportion with another PGIB strain, Asticcacaulis excentricus M6, regardless of the inoculation ratios (99:1–1:99) and inoculation order. We also found that A. magnusonii H3 exerted its growth-promoting effect over the negative effects of the two PGIB strains even when only a small amount was inoculated, probably due to its excellent competitive colonization ability. These experimental results demonstrate that there is a constant ecological equilibrium state involved in the bacterial colonization of aquatic plants.

Keywords

Duckweed Plant growth-promoting bacteria Plant growth-inhibiting bacteria Colonization Competition Aquatic plant 

Notes

Acknowledgments

We thank Dr. Takao Sakata and Ms. Minami Tada for their support in experiments.

Funding Information

This work was supported by the Advanced Low Carbon Technology Research and Development Program Grant Number JPMJAL1108 of the Japan Science and Technology Agency and the Japan Society for the Promotion of Science KAKENHI Grant Number JP18J10181. A part of this work was supported by the Advanced Characterization Nanotechnology Platform, Nanotechnology Platform Program of the Ministry of Education, Culture, Sports, Science and Technology, Japan, at the Research Center for Ultra-High Voltage Electron Microscopy (Nanotechnology Open Facilities) in Osaka University.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

248_2018_1306_MOESM1_ESM.docx (16 kb)
Supplementary Table 1 (DOCX 16 kb)
248_2018_1306_MOESM2_ESM.xlsx (13 kb)
Supplementary Table 2 (XLSX 13 kb)

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Copyright information

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Authors and Affiliations

  1. 1.Division of Sustainable Energy and Environmental Engineering, Graduate School of EngineeringOsaka UniversityOsakaJapan
  2. 2.Research Center for Ultra-High Voltage Electron MicroscopyOsaka UniversityOsakaJapan
  3. 3.Division of Biosphere Science, Graduate School of Environmental ScienceHokkaido UniversitySapporoJapan

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