Microbial Ecology

, Volume 75, Issue 1, pp 113–122 | Cite as

A Combination of Stable Isotope Probing, Illumina Sequencing, and Co-occurrence Network to Investigate Thermophilic Acetate- and Lactate-Utilizing Bacteria

  • Weimin SunEmail author
  • Valdis Krumins
  • Yiran Dong
  • Pin GaoEmail author
  • Chunyan Ma
  • Min Hu
  • Baoqin Li
  • Bingqing Xia
  • Zijun He
  • Shangling Xiong
Environmental Microbiology


Anaerobic digestion is a complicated microbiological process that involves a wide diversity of microorganisms. Acetate is one of the most important intermediates, and interactions between acetate-oxidizing bacteria and archaea could play an important role in the formation of methane in anoxic environments. Anaerobic digestion at thermophilic temperatures is known to increase methane production, but the effects on the microbial community are largely unknown. In the current study, stable isotope probing was used to characterize acetate- and lactate-oxidizing bacteria in thermophilic anaerobic digestion. In microcosms fed 13C-acetate, bacteria related to members of Clostridium, Hydrogenophaga, Fervidobacterium, Spirochaeta, Limnohabitans, and Rhodococcus demonstrated elevated abundances of 13C-DNA fractions, suggesting their activities in acetate oxidation. In the treatments fed 13C-lactate, Anaeromyxobacter, Desulfobulbus, Syntrophus, Cystobacterineae, and Azospira were found to be the potential thermophilic lactate utilizers. PICRUSt predicted that enzymes related to nitrate and nitrite reduction would be enriched in 13C-DNA fractions, suggesting that the acetate and lactate oxidation may be coupled with nitrate and/or nitrite reduction. Co-occurrence network analysis indicated bacterial taxa not enriched in 13C-DNA fractions that may also play a critical role in thermophilic anaerobic digestion.


Acetate oxidizing bacteria Thermophilic anaerobic digestion Stable isotope probing Illumina sequencing 



This research was funded by the High-level Leading Talent Introduction Program of GDAS, SPICC Program (2016GDASPT-0105), Construction of Innovative Talents for Pollution Control and Management of Heavy Metals in Farmland (2016B070701015), Innovation-driven Development Capability Construction Program of GDAS (2017GDASCX-0106), the Natural Science Foundation of Shanghai (17ZR1400400), the Fundamental Research Funds for the Central Universities (Donghua University), the DHU Distinguished Young Professor Program (Donghua University), and the Natural Science Foundation of Zhejiang Province (LY12C01003).

Supplementary material

248_2017_1017_MOESM1_ESM.docx (40 kb)
ESM 1 (DOCX 40 kb).


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and ManagementGuangdong Institute of Eco-environment Science and TechnologyGuangzhouChina
  2. 2.Department of Environmental SciencesRutgers UniversityNew BrunswickUSA
  3. 3.Carl R. Woese Institute for Genomic BiologyUniversity of Illinois, Urbana-ChampaignUrbanaUSA
  4. 4.College of Environmental Science and Engineering, State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile IndustryDonghua UniversityShanghaiChina
  5. 5.Biological and Environmental CollegeZhejiang Wanli UniversityNingboChina

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