Genotoxic Evaluation in Tadpoles Associated with Agriculture in the Central Cerrado, Brazil

  • Rinneu Elias BorgesEmail author
  • Lia Raquel de Souza Santos
  • Marcelino Benvindo-Souza
  • Richelle Sousa Modesto
  • Rhayane Alves Assis
  • Classius de Oliveira


Many agricultural practices cause environmental degradation that affects the cellular integrity of anurans. In the present study, we provided in situ data of Dendropsophus minutus, Physalaemus cuvieri, and Scinax fuscovarius collected in soybean/corn and conservation units in the Brazilian Cerrado. The in situ data showed no significant variation in the micronucleus frequency between the sites, only the reniform cells had a higher rate for the agricultural environment. A combined analysis of all nuclear erythrocyte abnormalities (ENAs = nuclear buds, reniform nuclei, apoptotic cell, binucleated, and anucleated cells) was recorded higher frequencies in farmland. Overall, Scinax fuscovarius was considered the best potential bioindicator for soybean/corn plantations. Finally, we recommend expanding the micronucleus test for in situ studies to expand our understanding of the sensitivity of native anuran species and provide a more systematic assessment of the adverse effects of environmental pollutants on wildlife.



The authors are grateful to the Brazilian National Council for Scientific and Technological Development (CNPq), for supporting this study through personal research Grant Number 477044/2013-1, and the São Paulo State Research Foundation (FAPESP) for supporting the analyses (Process No. 2013/02067-5). They also thank the Chico Mendes Institute for Biodiversity Conservation (ICMBio/SISBIO) and the Emas National Park for authorizing fieldwork.

Supplementary material

244_2019_623_MOESM1_ESM.docx (33 kb)
Supplementary material 1 (DOCX 32 kb)


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of BiologyUniversidade de Rio Verde, UniRVRio VerdeBrazil
  2. 2.Laboratory of Animal BiologyInstituto Federal Goiano, IF GoianoRio VerdeBrazil
  3. 3.Department of BiologyUniversidade Estadual Paulista - Júlio de Mesquita Filho, UNESPSão José do Rio PretoBrazil

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