Skin wound healing properties of Hypericum perforatum, Liquidambar orientalis, and propolis mixtures
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Traditional formulation therapies based on natural origin compounds offer new alternatives for treatment of skin wounds. Hypericum perforatum (HP), Liquidambar orientalis (LO), and propolis have been proved to promote skin wound healing. Extracts of these compounds are traditionally used as folk remedies. They all have different effects on each phases of wound healing. Wound healing effects of the mixtures of these compounds were investigated. HP, LO, and propolis were prepared as combinations of mixtures at an equal rate. Fifty Spraque-Dawley rats were included in this study. They were divided into the following 5 groups: group 1 (control), group 2 (HP-propolis, 1:1), group 3 (HP-LO, 1:1), group 4 (LO-propolis, 1:1), and group 5 (HP-LO-propolis, 1:1:1). Two incisional wounds were made and primarily closed on the interscapular region of every rat. Formulations were applied daily on the wounds. Biopsies were taken on days 3, 7, and 21 postoperatively from every rat. Histopathological and tensile strength parameters were analyzed. Angiogenesis and epithelialization rates were significantly higher in treatment groups compared with control (p < 0.05). Inflammation was significantly lower (p < 0.05) in treatment groups compared with control. There was no significance in tensile strength between groups. There was no difference between treatment groups. Hypericum perforatum, Liquidambar orientalis, and propolis have all improved wound healing in incisional wounds. Although they produced different effects on various parameters, mixtures of these compounds ensure a more stable response to wounds. As a result, we can mention about a positive synergy between the compounds. Level of Evidence: Level III, Experimental study.
KeywordsCombination therapy Hypericum perforatum Liquidambar orientalis Propolis Traditional therapy Wound healing
This study was supported by the Funding of Scientific Research Projects of Muğla Sıtkı Koçman University Grant No: 17/045.
Compliance with ethical standards
Conflict of interest
Mehmet Altıparmak, Mustafa Kule, Yasin Öztürk, Serkan Y. Çelik, Mehmet Öztürk, Mehmet E. Duru, and Uğur Koçer declare that they have no conflict of interest.
This study was ethically approved by Ankara Education and Research Hospital Ethical Committee No: 0030 Decision: 403.
- 4.Schempp CM, Windeck T, Hezel S, Simon JC (2003) Topical treatment of atopic dermatitis with St. John’s wort cream--a randomized, placebo controlled, double blind half-side comparison. Phytomedicine 10(Suppl 4):31–37 Available at: http://www.ncbi.nlm.nih.gov/pubmed/12807340. Accessed: 11 April 2018CrossRefGoogle Scholar
- 7.Maisenbacher P, Kovar KA (1992) Analysis and stability of Hyperici oleum. Planta Med 58(4):351–354Google Scholar
- 10.Irina Prisăcaru A et al (2013) Evaluation of the wound-healing effect of a novel Hypericum perforatum ointment in skin injury. Romanian J Morphol Embryol 54(4):1053–1059 Available at: http://www.rjme.ro/RJME/resources/files/54041310531059.pdf. Accessed: 27 February 2018Google Scholar
- 11.Han MC et al (2005) Effects of Turkish propolis and silver sulfadiazine on burn wound healing in rats. Rev Med Vet 156(12):624–627Google Scholar
- 12.Luiz Cavalcanti de Albuquerque-Júnior R et al (2009) Effect of bovine type-I collagen-based films containing red propolis on dermal wound healing in rodent model Efecto de Películas a Base de Colágeno Bovino Tipo I que Contienen Propóleo Rojo sobre la Cicatrización de la Herida Cutánea en un Modelo Roedor. Int J Morphol 27(4):1105–1110 Available at: https://pdfs.semanticscholar.org/a45e/d45dea1d54c6688f3d35af2a7c2c07dd4d6d.pdf. Accessed: 11 April 2018Google Scholar
- 14.Song YS, Park EH, Jung KJ, Jin C (2002) Inhibition of angiogenesis by Propolis. Arch Pharm Res 25(4):500–504 Available at: https://link.springer.com/content/pdf/10.1007%2FBF02976609.pdf. Accessed: 23 April 2018CrossRefGoogle Scholar
- 16.Henshaw FR, Bolton T, Nube V, Hood A, Veldhoen D, Pfrunder L, McKew GL, Macleod C, McLennan SV, Twigg SM (2014) Topical application of the bee hive protectant propolis is well tolerated and improves human diabetic foot ulcer healing in a prospective feasibility study. J Diabetes Complicat 28(6):850–857CrossRefGoogle Scholar
- 19.Ökmen G, Cantekin Z, Alam MI, Türkcan O, Ergün Y (2017) Antibacterial and antioxidant activities of Liquidambar orientalis mill. various extracts against bacterial pathogens causing mastitis. Turk J Agric Food Sci Technol [s.n.] 5. https://doi.org/10.24925/turjaf.v5i8.883-887.1163
- 24.Yanik ME et al (2016) Comparison of topical treatment with silver sulfadiazine and sweetgum oil (Liquidambar orientalis) on burn wound healing in an experimental rat model. Anal Quant Cytol Histol 38(3):168–174Google Scholar
- 27.Kıyan S et al (n.d.) Investigation of acute effects of Hypericum perforatum (St. John’s Wort-Kantaron) treatment in experimental thermal burns and comparison with silver sulfadiazine treatment. https://doi.org/10.5505/tjtes.2015.63822