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The Journal of Membrane Biology

, Volume 253, Issue 1, pp 1–10 | Cite as

Effect of Triclosan on the Functioning of Liver Mitochondria and Permeability of Erythrocyte Membranes of Marsh Frog (Pelophylax ridibundus (Pallas, 1771))

  • Mikhail V. DubininEmail author
  • Kirill S. Tenkov
  • Anton O. Svinin
  • Victor N. Samartsev
  • Konstantin N. Belosludtsev
Article
  • 173 Downloads

Abstract

The paper examines the effects of the antimicrobial agent triclosan on the functioning of the liver mitochondria of marsh frog (Pelophylax ridibundus (Pallas, 1771)). It was established that triclosan inhibits DNP-stimulated respiration of mitochondria and decreases respiratory control ratio. In addition, triclosan causes the collapse of the mitochondrial membrane potential on both types of substrates. Such an action of triclosan can be mediated by both a protonophore effect and suppression of the activity of complex II and combined activity of complexes II + III (and, to a lesser degree, the combined activity of complexes I + III) of the mitochondrial respiratory chain. It is shown that high concentrations of triclosan enhance the production of hydrogen peroxide during the oxidation of substrates of the complex I by mitochondria, and decrease it in the case of succinate oxidation. It is found that triclosan is able to induce nonspecific permeability of the liver mitochondria of these amphibians, as well as the plasma membrane of erythrocytes. The possible mechanisms of triclosan effect on marsh frog liver mitochondria and red blood cells are discussed.

Graphic Abstract

Keywords

Triclosan Toxicology Mitochondria Pelophylax ridibundus Erythrocytes Membrane permeability 

Abbreviations

TCS

Triclosan

CsA

Cyclosporine

MPT

Mitochondrial permeability transition

RBC

Red blood cells

Notes

Acknowledgements

The study was supported by grants from the Russian Foundation for Basic Research (18-315-00033) and the Ministry of Science and Higher Education of the Russian Federation (17.4999.2017/8.9 and 6.5170.2017/8.9).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Mari State UniversityYoshkar-OlaRussia
  2. 2.Institute of Theoretical and Experimental BiophysicsRussian Academy of SciencesMoscowRussia

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