Enterodiol is Actively Transported by Rat Liver Cell Membranes
The interaction of enterodiol and the well-described polyphenol epigallocatechin gallate (EGCG) with hepatic membranes has been matter of interest in the last few years. On one hand, EGCG is only able to bind to the phospholipid polar head groups, as it has been already described in synthetic lipid bilayers and erythrocyte membranes but cannot get inserted into the hydrophobic core or be transported into the lumen of membrane vesicles. On the other, enterodiol has no interaction with non-energized membranes either, but it is able to interact and even be transported upon addition of ATP. In fact, the ATPase activity undergoes a twofold increase in the presence of enterodiol but not in the presence of EGCG. This is the first report on the transport of enterodiol by liver membranes, and it may help explain the rather high blood concentrations of this estrogenic enterolignan compared to EGCG, which is extensively metabolized by the intestine and the liver. The present results suggest that a fraction of enterodiol may escape the liver inactivation by being pumped out from the hepatocytes to the bloodstream.
KeywordsPolyphenols Membrane binding ATPase activity Lignans
This work was supported by CONICET (Grant PIP 0183), Agencia (Grant PICT 2012 N°2998), and UNT (Grant PIUNT 2014 D548/1). A.A.M.C. and P.B.S. are recipients of a CONICET fellowship. C.M. is a career investigator of CONICET. The authors would like to thank N.S. Rios Colombo for constructive criticism of the manuscript.
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- de Athayde Moncorvo Collado A, Dupuy FG, Morero RD, Minahk yC (2016) Cholesterol induces surface localization of polyphenols in model membranes thus enhancing vesicle stability against lysozyme, but reduces protection of distant double bonds from reactive-oxygen species. Biochim Biophys Acta 1858(7 Pt A):1479–1487CrossRefPubMedGoogle Scholar