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Heat and Mass Transfer

, Volume 49, Issue 11, pp 1647–1658 | Cite as

Three dimensional simulation on binding efficiency of immunoassay for a biosensor with applying electrothermal effect

  • Kuan-Rong HuangEmail author
  • Jeng-Shian Chang
Original

Abstract

In this work, we perform three dimensional finite element simulations on the binding reaction kinetics of the commonly used analyte–ligand protein pairs, namely, C-reactive protein (CRP) and anti-CRP, in a reaction chamber (microchannel) of a biosensor. For the diffusion limited binding biomolecular pairs, due to the slower transport speed of the analyte and the faster reaction rate of analyte–ligand complex, diffusion boundary layers often develop on the reaction surface. To enhance the performance of a biosensor by accelerating the transport speed, a non-uniform AC electric field is applied to induce the electrothermal force to stir the flow field. The swirling flow in the fluid can accelerate the transport of the analyte to and from the reaction surface and hence enhance the association and dissociation of analyte–ligand complex. Four types of biosensors with different arrangements of the geometric locations of the electrode pair and the reaction surface are designed to study the effects of varying geometric configuration on the binding efficiency. The simulation results show that the performance of a biosensor can be better improved by placing the electrodes and the reaction surface on the same side of the microchannel against the opposite side. For the best case studied in this work, the maximum initial slope of the binding curve can be raised up to 6.94 times (with respect to the field-free value) in the association phase, under applying AC field of 15 Vrms and operating frequency of 100 kHz. Another important result with applying electrothermal effect is that it is feasible to use the slower sample flow in the microchannel to save a lot of sample consumption without sacrificing the performance of a biosensor. Several control factors not studied in our previous works such as the thermal boundary condition and the effect of electrical conductivity are also discussed.

Keywords

Applied Voltage Reaction Surface Protein Pair Binding Reaction Electrode Pair 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by the National Science Council in Taiwan through NSC 97-2221-E-002-017-MY3 and National Taiwan University CQSE 97R0066-69. We thank the NCHC in Taiwan for providing computing resources.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Mechanical and System Engineering ProgramInstitute of Nuclear Energy ResearchTaoyuanTaiwan
  2. 2.Institute of Applied MechanicsNational Taiwan UniversityTaipeiTaiwan

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