Marine Biology

, 167:4 | Cite as

Regional comparison of leatherback sea turtle maturation attributes and reproductive longevity

  • Larisa AvensEmail author
  • Lisa R. Goshe
  • George R. Zug
  • George H. Balazs
  • Scott R. Benson
  • Heather Harris
Original Paper


Data characterizing somatic growth patterns and the ages and sizes at which organisms mature are fundamental to understanding population dynamics. However, obtaining this information for endangered leatherback sea turtles (Dermochelys coriacea) is particularly challenging due to unusual physiology and prevalence of remote oceanic habitat use, which limit direct observation. While inference has been made through indirect approaches such as captive, genetic, and/or skeletal growth mark (skeletochronology) studies, these diverse methods have yielded similarly varied results, limiting usefulness of available information for management and conservation. To address this data gap, we conducted refined skeletochronological analysis of Atlantic and Pacific leatherback scleral ossicle bones, allowing estimation of carapace length-at-age relationships throughout individual turtles’ lives, including the juvenile life stage. In addition, this improved approach made it possible to estimate mean and range for age and size at sexual maturation (ASM and SSM, respectively), as well as post-maturation longevity. Updated mean ASM estimates from the current study of 17–19 years were lower than those previously proposed using skeletochronology and more similar to predictions from captive growth and genetic data. Maximum estimates of reproductive longevity (18–22 years) were consistent with the 16–19 years reported previously from mark–recapture of nesting females. Together, these results indicate that the application of the refined analytical approach described in the current study may offer opportunities to increase understanding of leatherback age and growth.



We are grateful for the sample and data collection conducted by the participants in the National Sea Turtle Stranding and Salvage Network, the NMFS Pacific Islands Fisheries Science Center’s Marine Turtle Biology and Assessment Program, and the NMFS Pacific Islands Regional Office Observer Program, without which this study would not have been possible. The research and manuscript were improved through discussions with and comments from A. Chester, M. Godfrey, A. Hohn, K. Stewart, and two anonymous reviewers.


The authors have no sources of funding to report for this study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Human and animal rights statement

All procedures performed in the current study were approved and permitted by the United States Fish and Wildlife Service (permit no. TE-67379 issued to the National Marine Fisheries Service Southeast Fisheries Science Center).


  1. Avens L, Snover ML (2013) Age and age estimation in sea turtles. In: Musick JA, Wyneken J, Lohmann KJ (eds) Biology of Sea Turtles, vol III. CRC Press, Boca Raton, pp 97–134CrossRefGoogle Scholar
  2. Avens L, Taylor JC, Goshe LR, Jones TT, Hastings M (2009) Use of skeletochronological analysis to estimate the age of leatherback sea turtles Dermochelys coriacea in the western North Atlantic. Endanger Species Res 8:165–177CrossRefGoogle Scholar
  3. Avens L, Goshe LR, Harms CA, Anderson ET, Hall AG, Cluse WM, Godfrey MH, Braun-McNeill J, Stacy B, Bailey R, Lamont MM (2012) Population characteristics, age structure, and growth dynamics of neritic juvenile green turtles in the northeastern Gulf of Mexico. Mar Ecol Prog Ser 458:213–229CrossRefGoogle Scholar
  4. Avens L, Goshe LR, Pajuelo M, Bjorndal KA, MacDonald BD, Lemons GE, Bolten AB, Seminoff JA (2013) Complementary skeletochronology and stable isotope analyses offer new insight into juvenile loggerhead oceanic stage duration and growth dynamics. Mar Ecol Prog Ser 491:235–251CrossRefGoogle Scholar
  5. Avens L, Goshe LR, Coggins L, Snover ML, Pajuelo M, Bjorndal KA, Bolten AB (2015) Age and size at maturation and adult stage duration for loggerhead sea turtles in the western North Atlantic. Mar Biol 162:1749–1767CrossRefGoogle Scholar
  6. Avens L, Goshe LR, Coggins L, Shaver DJ, Higgins B, Landry AM Jr, Bailey R (2017) Variability in age and size at maturation, reproductive longevity, and long-term growth dynamics for Kemp’s ridley sea turtles in the Gulf of Mexico. PLoS One 12(3):e0173999. CrossRefPubMedPubMedCentralGoogle Scholar
  7. Bels B, Rimbolt-Baly F, Lescure J (1988) Croissance et maintien en captivité, de la tortue luth Dermochelys coriacea (Vandelli, 1761). Rev Fr Aquariol 15:59–64Google Scholar
  8. Benson SR, Tapilatu RF, Pilcher N, Santidrián Tomillo P, Sarti Martínez L (2015) Leatherback turtle populations in the Pacific Ocean. In: Spotila JR, Tomillo PS (eds) The leatherback turtle: biology and conservation. Johns Hopkins University Press, Baltimore, pp 110–122Google Scholar
  9. Birkenmeier E (1971) Juvenile leathery turtles, Dermochelys coriacea (Linnaeus), in captivity. Brunei Mus J 2:160–172Google Scholar
  10. Bjorndal KA, Parsons J, Mustin W, Bolten AB (2013) Threshold to maturity in a long-lived reptile: interactions of age, size, and growth. Mar Biol 160:607–616. CrossRefGoogle Scholar
  11. Bjorndal KA, Parsons J, Mustin W, Bolten AB (2014) Variation in age and size at sexual maturity in Kemp’s ridley sea turtles. Endanger Species Res 25:57–67. CrossRefGoogle Scholar
  12. Bostrom BL, Jones TT, Hastings M, Jones DR (2010) Behaviour and physiology: the thermal strategy of leatherback turtles. PLoS One 5(11):e13925. CrossRefPubMedPubMedCentralGoogle Scholar
  13. Caillouet CW Jr, Shaver DJ, Landry AM Jr, Owens DW, Pritchard PCH (2011) Kemp’s ridley sea turtle (Lepidochelys kempii) age at first nesting. Chelonian Conserv Biol 10:288–293CrossRefGoogle Scholar
  14. Chan E-H, Liew H-C (1996) Decline of the leatherback population in Terengganu, Malaysia, 1956–1995. Chelonian Conserv Biol 2:196–203Google Scholar
  15. Deraniyagala PEP (1952) A colored atlas of some vertebrates from Ceylon, vol I. The Ceylon Government Press, Colombo, pp 3–21Google Scholar
  16. Dodge KL, Logan JM, Lutcavage ME (2011) Foraging ecology of leatherback sea turtles in the Western North Atlantic determined through multi-tissue stable isotope analyses. Mar Biol 158:2813–2824CrossRefGoogle Scholar
  17. Duchene S, Frey A, Alfaro-Núñez A, Dutton PH, Gilbert TP, Morin PA (2012) Marine turtle mitogenome phylogenetics and evolution. Mol Phylogenet Evol 65:241–250PubMedCrossRefGoogle Scholar
  18. Dutton PH, Bowen BW, Owens DW, Barragan A, Davis SK (1999) Global phylogeography of the leatherback turtle (Dermochelys coriacea). J Zool Lond 248:397–409CrossRefGoogle Scholar
  19. Dutton DL, Dutton PH, Chaloupka M, Boulon RH (2005) Increase of a Caribbean leatherback turtle Dermochelys coriacea nesting population linked to long-term nest protection. Biol Conserv 126:186–194CrossRefGoogle Scholar
  20. Eckert SA (2002) Distribution of juvenile leatherback sea turtle Dermochelys coriacea sightings. Mar Ecol Prog Ser 230:289–293CrossRefGoogle Scholar
  21. Eckert KL, Wallace BP, Frazier JG, Eckert SA, Pritchard PCH (2012) Synopsis of the biological data on the leatherback sea turtle (Dermochelys coriacea). Biological Technical Publication BTP-R4015-2012 USFWSGoogle Scholar
  22. El Mouden PE, Francillon-Viellot HJ, Castanet J, Znari M (1997) Age individual, maturite, croissance et longevite chez l’agamide nord-african, Agama impalearis Boettger, 1874, etudies a l’aide de la squelettochronologie. Ann Sci Nat Zool Paris 18:63–70Google Scholar
  23. Forestry Division (2010) (Government of the Republic of Trinidad and Tobago), Save our Seaturtles-Tobago, and Nature Seekers. WIDECAST Sea Turtle Recovery Action Plan for Trinidad & Tobago (KL Eckert, Ed). CEP Technical Report No. 49. UNEP Caribbean Environment Programme. Kingston, Jamaica. Xx + 132 pGoogle Scholar
  24. Francillon-Viellot H, Arntzen JW, Géraudie J (1990) Age, growth, and longevity of sympatric Triturus cristatus, T. marmoratus and their hybrids (Amphibia, Urodela): a skeletochronological comparison. J Herpetol 24:13–22CrossRefGoogle Scholar
  25. Frazer NB, Ehrhart LM (1985) Preliminary growth models for green, Chelonia mydas, and loggerhead, Caretta caretta, turtles in the wild. Copeia 1985:73–79CrossRefGoogle Scholar
  26. Gaspar P, Benson SR, Dutton PH, Réveillère A, Jacob G, Meetoo C, Dehecq A, Fossette S (2012) Oceanic dispersal of juvenile leatherback turtles: going beyond passive drift modeling. Mar Ecol Prog Ser 457:265–284CrossRefGoogle Scholar
  27. Gillespie D, Caillat M, Gordon J (2013) Automatic detection and classification of odontocete whistles. J Acoust Soc America 134:2427–2437CrossRefGoogle Scholar
  28. Girondot M, Fretey J (1996) Leatherback turtles, Dermochelys coriacea, nesting in French Guiana, 1978–1995. Chelonian Conserv Biol 2:204–208Google Scholar
  29. Goshe LR, Avens L, Scharf FS, Southwood AL (2010) Estimation of age at maturation and growth of Atlantic green turtles (Chelonia mydas) using skeletochronology. Mar Biol 257:1725–1740CrossRefGoogle Scholar
  30. Goshe LR, Snover ML, Hohn AA, Balazs GH (2016) Validation of back-calculated body lengths and timing of growth mark deposition in Hawaiian green sea turtles. Ecol Evol 6:3208–3215PubMedPubMedCentralCrossRefGoogle Scholar
  31. Guarino FM, Di Giá I, Sindaco R (2008) Age structure in a declining population of Rana temporaria from northern Italy. Acta Zool Acad Sci Hung 54:99–112Google Scholar
  32. Huang H-W (2015) Conservation hotspots for the turtles on the high seas of the Atlantic Ocean. PLoS One 10(8):e0133614. CrossRefPubMedPubMedCentralGoogle Scholar
  33. Humburg NI, Balazs GH (2014) Forty years of research: recovery records of green turtles observed or originally tagged at French Frigate Shoals in the Northwestern Hawaiian Islands, 1973–2013. NOAA Technical Memorandum, NOAA-TM-NMFS-PIFSC-40Google Scholar
  34. Jones TT, Hastings MD, Bostrom BL, Pauly D, Jones DR (2011) Growth of captive leatherback turtles, Dermochelys coriacea, with inferences on growth in the wild: implications for population decline and recovery. J Exp Mar Biol Ecol 399:84–92CrossRefGoogle Scholar
  35. Lalire M, Gaspar P (2019) Modeling the active dispersal of juvenile leatherback turtles in the North Atlantic Ocean. Mov Ecol 7:7. CrossRefPubMedPubMedCentralGoogle Scholar
  36. Lee Lum L (2006) Assessment of incidental sea turtle catch in the artisanal gillnet fishery in Trinidad and Tobago, West Indies. Appl Herpetol 3:357–368CrossRefGoogle Scholar
  37. Lutcavage ME, Lutz PL (1997) Diving behavior. In: Lutz PL, Musick JA (eds) The biology of sea turtles. CRC Press, Boca Raton, pp 277–296Google Scholar
  38. Musick JA, Limpus CJ (1997) Habitat utilization and migration in juvenile sea turtles. In: Lutz PL, Musick JA (eds) The biology of sea turtles, vol 1. CRC Press. Boca Raton, FL, pp 137–164Google Scholar
  39. NMFS (National Marine Fisheries Service) and USFWS (U.S. Fish and Wildlife Service) (2013) Leatherback sea turtle (Dermochelys coriacea) 5-year review: summary and evaluation. National Marine Fisheries Service, Silver Spring, p 89Google Scholar
  40. Northwest Atlantic Leatherback Working Group (2018) Northwest Atlantic Leatherback Turtle (Dermochelys coriacea) Status Assessment (B Wallace, K Eckert, Compilers and Editors). Conservation Science Partners and the Wider Caribbean Sea Turtle Conservation Network (WIDECAST). WIDECAST Technical Report No. 16, Godfrey, Illinois, USA. 36 p Accessed 29 Nov 2018
  41. Plotkin P (2003) Adult migrations and habitat use. In: Lutz P, Musick JA, Wyneken J (eds) The biology of sea turtles, vol 2. CRC Press, Boca Raton, pp 225–242Google Scholar
  42. Pritchard PCH (2015) Introduction: phylogeny and evolutionary biology of the leatherback turtle. In: Spotila JR, Tomillo PS (eds) The leatherback turtle: biology and conservation. Johns Hopkins University Press, Baltimore, pp 3–7Google Scholar
  43. Ramirez MD, Avens L, Goshe LR, Seminoff J, Heppell SS (2015) Patterns of loggerhead turtle ontogenetic shifts revealed through isotopic analysis of annual skeletal growth increments. Ecosphere 6:1–17CrossRefGoogle Scholar
  44. Ramirez M, Avens L, Seminoff JA, Goshe LR, Heppell SS (2017) Growth dynamics of loggerhead sea turtles undergoing an ontogenetic habitat shift. Oecologia 183:1087. CrossRefPubMedPubMedCentralGoogle Scholar
  45. Ramirez MD, Miller JA, Parks E, Avens L, Goshe LR, Seminoff JA, Snover ML, Heppell SS (2019) Reconstructing sea turtle ontogenetic habitat shifts through trace element analysis of bone tissue. Mar Ecol Prog Ser 608:247–262. CrossRefGoogle Scholar
  46. Rhodin AGJ (1985) Comparative chondro-osseous development and growth of marine turtles. Copeia 1985:752–771CrossRefGoogle Scholar
  47. Saba VS, Shillinger GL, Swithenbank AM, Block BA, Spotila JR, Musick JA, Paladino FV (2008) An oceanographic context for the foraging ecology of eastern Pacific leatherback turtles: consequences of ENSO. Deep Sea Res I 55:646–660CrossRefGoogle Scholar
  48. Sarti Martínez L, Barragán AR, Muñoz DG, García N, Huerta P, Vargas F (2007) Conservation and biology of the leatherback turtle in the Mexican Pacific. Chelonian Conserv Biol 6:70–78CrossRefGoogle Scholar
  49. Scott R, Biastoch A, Agamboue PD, Bayer T, Boussamba FL, Formia A, Godley BJ, Mabert BDK, Manfoumbi JC, Schwarzkopf FU, Sounguet G-P, Wagner P, Witt MJ (2017) Spatio-temporal variation in ocean current-driven hatchling dispersion: implications for the world’s largest leatherback sea turtle nesting region. Divers Distrib 23:604–614CrossRefGoogle Scholar
  50. Snover ML, Avens L, Hohn AA (2007) Back-calculating length from skeletal growth marks in loggerhead sea turtles Caretta caretta. Endanger Species Res 3:95–104CrossRefGoogle Scholar
  51. Stewart K, Johnson C, Godfrey MH (2007) The minimum size of leatherbacks at reproductive maturity, with a review of sizes for nesting females from the Indian, Atlantic, and Pacific Ocean basins. Herpetol J 17:123–128Google Scholar
  52. Stewart K, Sims M, Meylan A, Witherington B, Brost B, Crowder LB (2011) Leatherback nests increasing significantly in Florida, USA; trends assessed over 30 years using multilevel modeling. Ecol Appl 21:263–273PubMedCrossRefPubMedCentralGoogle Scholar
  53. Steyermark AC, Williams K, Spotila JR, Paladino FV, Rostal DC, Morreale SJ, Koberg MT, Arauz R (1996) Nesting leatherback turtles at Las Baulas National Park, Costa Rica. Chelonian Conserv Biol 2:173–183Google Scholar
  54. Tapilatu RF, Dutton PH, Tiwari M, Wibbels T, Ferdinandus HV, Iwanggin WG, Nugroho BH (2013) Long-term decline of the western Pacific leatherback, Dermochelys coriacea: a globally important sea turtle population. Ecosphere 4:1–15CrossRefGoogle Scholar
  55. R Core Team (2017) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Accessed 28 Nov 2018
  56. TEWG (Turtle Expert Working Group) (2007) An assessment of the leatherback turtle population in the Atlantic Ocean. NOAA Technical Memorandum NMFS-SEFSC-555, p 116Google Scholar
  57. Tomillo PS, Vélez E, Reina RD, Piedra R, Paladino FV, Spotila JR (2007) Reassessment of the leatherback turtle (Dermochelys coriacea) nesting population at Parque Nacional Marino Las Baulas, Costa Rica: effects of conservation efforts. Chelonian Conserv Biol 6:54–62CrossRefGoogle Scholar
  58. Tucker AD, Frazer NB (1991) Reproductive variation in leatherback turtles, Dermochelys coriacea, at Culebra National Wildlife Refuge, Puerto Rico. Herpetologica 1991:115–124Google Scholar
  59. Turner Tomaszewicz CN, Seminoff JA, Peckham SH, Avens L, Kurle CM (2017) Intrapopulation variability in the timing of ontogenetic habitat shifts in sea turtles revealed using 15N values from bone growth rings. J Anim Ecol. CrossRefPubMedPubMedCentralGoogle Scholar
  60. Turner Tomaszewicz CN, Seminoff JA, Avens L, Goshe LR, Rodriguez-Baron JM, Peckham SH, Kurle CM (2018) Expanding the coastal forager paradigm: long-term pelagic habitat use by green turtles Chelonia mydas in the eastern Pacific Ocean. Mar Ecol Prog Ser 587:217–234CrossRefGoogle Scholar
  61. Van Buskirk J, Crowder LB (1994) Life-history variation in marine turtles. Copeia 1994:66–81CrossRefGoogle Scholar
  62. Wallace BP, Jones TT (2015) Leatherback turtle physiological ecology: implications for bioenergetics and population dynamics. In: Spotila JR, Tomillo PS (eds) The leatherback turtle: biology and conservation. Johns Hopkins University Press, Baltimore, pp 149–161Google Scholar
  63. Wallace BP, Saba VS (2009) Environmental and anthropogenic impacts on intra-specific variation in leatherback turtles: opportunities for targeted research and conservation. Endanger Species Res 7:11–21. CrossRefGoogle Scholar
  64. Wallace BP, Kilham SS, Paladino FV, Spotila JR (2006) Energy budget calculations indicate resource limitation in Eastern Pacific leatherback turtles. Mar Ecol Prog Ser 318:263–270CrossRefGoogle Scholar
  65. Wallace BP, Tiwari M, Girondot M (2013) Dermochelys coriacea. The IUCN Red List of Threatened Species 2013: e.T6494A43526147
  66. Wood SN (2006) Generalized additive models: an introduction with R. Chapman & Hall/CRC, Boca RatonCrossRefGoogle Scholar
  67. Zug GR, Parham JF (1996) Age and growth in leatherback turtles, Dermochelys coriacea (Testudines: Dermochelyidae): a skeletochronological analysis. Chelonian Conserv Biol 2:244–249Google Scholar

Copyright information

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2019

Authors and Affiliations

  1. 1.National Marine Fisheries Service, Southeast Fisheries Science CenterNOAA Beaufort LaboratoryBeaufortUSA
  2. 2.Department of Vertebrate ZoologyNational Museum of Natural History, Smithsonian InstitutionWashingtonUSA
  3. 3.Golden Honu Services of OceaniaHonoluluUSA
  4. 4.Marine Mammal and Turtle Division, Southwest Fisheries Science CenterNational Marine Fisheries Service, National Oceanic and Atmospheric AdministrationMoss LandingUSA
  5. 5.Moss Landing Marine LaboratoriesSan Jose State UniversityMoss LandingUSA
  6. 6.National Marine Fisheries ServiceNOAA West Coast RegionMorro BayUSA

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