Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging
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We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples.
KeywordsFluorescence Yield Photosynthetic Performance Actinic Light Rapid Light Curf Rapid Light Curf
This study was supported by grants from the Danish Natural Science Research Council (MK) and the Carlsberg Foundation (MK). We acknowledge the assistance of Egil Nielsen for mechanical constructions and the staff at the Heron Island Research Station for technical assistance and support.
- Jakob T, Schreiber U, Kirschesch V, Langner U, Wilhelm C (2005) Estimation of chlorophyll content and daily primary production of the major algal groups by means of multiwavelength-excitation PAM chlorophyll fluorometry: performance and methodological limits. Photosynth Res 83:343–361CrossRefGoogle Scholar
- Papageorgiou GC, Govindjee (2004) Chlorophyll fluorescence: a signature of photosynthesis. Kluwer Academic Publishers, DordrechtGoogle Scholar
- Xupeng HU, Rongguo SU, Zhang F, Wang X, Wang H, Zheng Z (2010) Multiple excitation wavelength fluorescence emission spectra technique for discrimination of phytoplankton. J Ocean Univ China (Ocean Coastal Sea Res) 9:16–24Google Scholar