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Marine Biology

, Volume 158, Issue 6, pp 1223–1231 | Cite as

Variations in cardiac performance and heat shock protein expression to thermal stress in two differently zoned limpets on a tropical rocky shore

  • Yun-wei Dong
  • Gray A. Williams
Original Paper

Abstract

Understanding variation in physiological adaptations to thermal stress is vital when investigating intertidal species’ distribution patterns. The thermal sensitivities of two limpets, Cellana grata and C. toreuma, differed in accordance with their vertical distributions. Cardiac performance was maintained at higher temperatures (~47°C) for the high-zone C. grata than the mid-zone C. toreuma (~42°C). At 40°C, C. grata maintained regular heart function for ~4 h, while heart function of C. toreuma decreased rapidly. Heat shock protein expression revealed that C. toreuma had two constitutive isoforms, Hsp77 and Hsp72, and C. grata one inducible form, Hsp75, which was upregulated at 40°C, suggesting C. grata has a more effective heat shock response than C. toreuma. The temperature-adaptive differences in cardiac thermal tolerance and Hsp expression match observed differences in thermally induced mortalities with the onset of summer and may help predict differential effects of climate change on the two congeners.

Keywords

Heat Stress Heat Shock Protein Heat Shock Response Cardiac Performance Rocky Shore 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

YD was supported by a Sino-British Fellowship Trust Visitorship awarded by The University of Hong Kong, National Natural Science Foundation of China (41076083) and the Fundamental Research Funds for the Central Universities (201012028). We thank Stephen Cartwright for his help in specimen collection, Wai Chuen Ng and Priscilla Leung (all SWIMS) for their help in heat shock protein assay and David Marshall (Universiti Brunei Darussalam) for his suggestions in heart rate measurement. We are also grateful to Tomoyuki Nakano (Museum of National History of Japan) for sharing information on the phylogeography of the two species and especially appreciate comments from Prof. George Somero (Hopkins Marine Station, Stanford University), Prof Hans-Otto Pörtner (Alfred Wegener Institute for Polar and Marine Research, Germany) and three referees, which have improved the manuscript.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.The State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science, Xiamen UniversityXiamenPeople’s Republic of China
  2. 2.The Swire Institute of Marine Science and Division of Ecology & Biodiversity, School of Biological Sciences, The University of Hong KongHong Kong SARPeople’s Republic of China

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