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Marine Biology

, Volume 156, Issue 8, pp 1625–1632 | Cite as

Bacterial symbionts as an additional cytological marker for identification of sponges without a skeleton

  • Andrey E. Vishnyakov
  • Alexander V. Ereskovsky
Original Paper

Abstract

Symbiotic bacteria from six Oscarella species (adults and embryos) collected in the Mediterranean Sea (O. lobularis, O. tuberculata, O. imperialis, O. microlobata, O. viridis) and the Sea of Japan (O. malakhovi) were investigated by scanning electron microscopy and transmission electron microscopy. In most cases, symbionts are rather numerous. Each sponge species has a definite set of bacterial morphological types. All bacteria are extracellular. Symbionts occupy the mesohyl of adult sponges or intercellular space in embryos and are often in contact with mesohylar filaments or cells. Bacteria of some morphotypes have characteristic blebs. Most symbionts are gram-negative, and two types of bacteria have traits of Archaea and one type of bacteria is similar to Planctomycetes. Data on morphology of bacterial symbionts can be a good additional character for identification of Oscarella species, which have no skeleton.

Keywords

Sponge Archaea Symbiotic Bacterium Sponge Species Bacterial Symbiont 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors are grateful to Chantal Bézac, Centre d’Océanologie de Marseille, France and Daria Tokina, Zoological Institute RAS, St. Petersburg, Russia, for technical assistance, Thierry Perez, Roland Graille and Pierre Chevaldonné for diving assistance. Financial support for this work was provided by grants RFBR NN 07-04-01097, 06-04-48660 and European Marie Curie Mobility program (fellowship of A. Ereskovsky, MIF1-CT-2006-040065).

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Andrey E. Vishnyakov
    • 1
  • Alexander V. Ereskovsky
    • 1
    • 2
  1. 1.Department of Invertebrate Zoology, Biological FacultySt. Petersburg State UniversitySt. PetersburgRussia
  2. 2.Centre d’Océanologie de Marseille, Station marine d’EndoumeAix-Marseille Université, CNRS UMR 6540-DIMARMarseilleFrance

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