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Marine Biology

, Volume 156, Issue 1, pp 25–38 | Cite as

Effect of reproduction on escape responses, metabolic rates and muscle mitochondrial properties in the scallop Placopecten magellanicus

  • Edouard Kraffe
  • Réjean Tremblay
  • Sonia Belvin
  • Jeqn-René LeCoz
  • Yanic Marty
  • Helga Guderley
Original Paper

Abstract

In scallops, gametogenesis and spawning can diminish the metabolic capacities of the adductor muscle and reduce escape response performance. To evaluate potential mechanisms underlying this compromise between reproductive investment and escape response, we examined the impact of reproductive stage (pre-spawned, spawned and reproductive quiescent) of the giant scallop, Placopecten magellanicus, on behavioural (i.e., escape responses), physiological (i.e., standard metabolic rates and metabolic rates after complete fatigue) and mitochondrial capacities (i.e., oxidative rates) and composition. Escape responses changed markedly with reproductive investment, with spawned scallops making fewer claps and having shorter responses than pre-spawned or reproductive-quiescent animals. After recuperation, spawned scallops also recovered a lower proportion of their initial escape response. Scallop metabolic rate after complete fatigue (VO2max) did not vary significantly with reproductive stage whereas standard metabolic rate (VO2min) was higher in spawned scallops. Thus spawned scallops had the highest maintenance requirements (VO2min/VO2max). Maximal capacities for glutamate oxidation by muscle mitochondria did not change with reproductive stage although levels of ANT and cytochromes as well as cytochrome C oxidase (CCO) activity did. Total mitochondrial phospholipids, sterols and the proportion of phospholipid classes differed only slightly between reproductive stages. Few modifications were detected in the fatty acid (FA) composition of the phospholipid classes except in cardiolipin (CL). In this class, pre-spawned and spawned scallops had fairly high proportions of 20:5n-3 whereas this FA in reproductive-quiescent scallops was threefold lower and 22:6n-3 was significantly higher. These changes paralleled the increases in CCO activity and suggest an important role of CL on the modifications of CCO activity in scallops. However, mitochondrial properties could not explain the decreased recuperation ability from exhausting exercise in spawned scallops. Shifts in maintenance requirements (VO2min/VO2max) and aerobic scope (VO2max − VO2min) provided the best explanation for the impact of reproduction on escape response performance.

Keywords

Reproductive Stage Escape Response Phospholipid Class Reproductive Investment Standard Metabolic Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

ANT

Adenine nucleotide translocase

AVF

Atresic volume fraction

BSA

Bovine serum albumin

CAT

Carboxyatractyloside

CL

Cardiolipin

CCO

Cytochrome C oxidase

diacylPC

Diacylphosphatidylcholine

diacylPE

Diacylphosphatidylethanolamine

GVF

Gamete volume fraction

GSI

Gonadosomatic index

MUFA

Monounsaturated fatty acids

PI

Phosphatidylinositol

PS

Phosphatidylserine

PlsmPE

Plasmalogen ethanolamine

PUFA

Polyunsaturated fatty acids

SFA

Saturated fatty acids

SMR

Standard metabolic rates

Notes

Acknowledgments

This research was supported by a grant from NSERC to HG. EK received post-doctoral support from the Reseau Aquacole du Québec and from the Université de Bretagne Occidentale. The direction of innovation and technology of the Ministère de l’Agriculture, des Pêcheries et de l’Alimentation du Québec (MAPAQ) allowed accessibility and metabolic measurements at CAMGR.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Edouard Kraffe
    • 1
    • 2
  • Réjean Tremblay
    • 3
  • Sonia Belvin
    • 3
  • Jeqn-René LeCoz
    • 4
  • Yanic Marty
    • 1
  • Helga Guderley
    • 2
  1. 1.Unité mixte CNRS 6521Université de Bretagne OccidentaleBrest Cedex 3France
  2. 2.Département de BiologieUniversité LavalQuebecCanada
  3. 3.Institut des Sciences de la MerRimouskiCanada
  4. 4.UMR 100 Physiologie et Ecophysiologie des Mollusques Marins, IfremerCentre de BrestPlouzanéFrance

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