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Marine Biology

, Volume 155, Issue 4, pp 353–361 | Cite as

δ13C and δ15N values in reef corals Porites lutea and P. cylindrica and in their epilithic and endolithic algae

  • Eduard A. Titlyanov
  • Serguei I. Kiyashko
  • Tamara V. Titlyanova
  • Tatyana L. Kalita
  • John A. Raven
Original Paper

Abstract

In summer 1998, shallow water corals at Sesoko Island, Japan (26°38′N, 127°52′E) were damaged by bleaching. In August 2003, partially damaged colonies of the massive Porites lutea and the branching P. cylindrica were collected at depths of 1.0–2.5 m. The species composition of epilithic algal communities on dead skeletal surfaces of the colonies (‘red turfs’, ‘green turfs’, ‘red crusts’) and the endolithic algae (living in coral skeletons) growing close to and away from living coral polyps was determined. Carbon and nitrogen stable isotope values of organic matter (δ13C and δ15N) from all six of these biological entities were determined. There were no significant differences in the isotope composition of coral tissues of the two corals, with P. lutea having δ13C of −15.3 to −9.6‰ and δ15N of 4.7–6.1‰ and P. cylindrica having similar values. Polyps in both species living close to an interface with epilithic algae had similar isotope values to polyps distant from such an interface. Despite differences in the relative abundance of the algal species in red turfs and crusts, their δ13C and δ15N values were not significantly different from each other (−18.2 to −13.9, −20.6 to −16.2, 1.1–4.3, and 3.3 to 4.9‰, respectively). The green algal turf had significantly higher δ13C values (−14.9 to −9.3‰) than that of red turfs and crusts but similar δ15N (1.2–4.1‰) to the red algae. The data do not suggest that adjoining associations of epilithic algae and coral polyps exchange carbon- and nitrogen-containing metabolites to a significant extent. The endolithic algae in the coral skeletons had δ13C values of −14.8 to −12.3‰ and δ15N of 4.0–5.4‰. Thus they did not differ significantly from the coral polyps in their carbon and nitrogen isotope values. The similarity in carbon isotope values between the coral polyps and endolithic algae may be attributed to a common source of CO2 for zooxanthellae and endolithic algae, namely, from respiration by the coral host. While it is difficult to fully interpret similarity in the nitrogen isotope composition of coral tissue and of green endolithic algae and the difference in δ15N between green epilithic and endolithic algae, the data are consistent with nitrogen-containing metabolites from the scleractinian coral serving as a significant source of nitrogen for the endolithic algae.

Keywords

Dissolve Inorganic Carbon Dissolve Inorganic Nitrogen Dissolve Organic Nitrogen Coral Species Coral Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by the State Program ‘Comprehensive analyses on biodiversity in coral reef and island ecosystems in Asian and Pacific regions’ (Japan, Leader of the program Prof. Makoto Tsuchiya). We are grateful to all staff at the Sesoko Station (Ryukyu University) for use of facilities, technical help, hospitality, and facilitation of research work. The University of Dundee is a registered Scottish charity, No. SC015096.

References

  1. Allemand D, Ferrier-Pages C, Furla P, Houlbreque F, Puverel S, Reynaud S et al (2004) Biomineralisation in reef-building corals: from molecular mechanisms to environmental control. Comptes Rendus Palevol Acad Sci Paris 3:453–467CrossRefGoogle Scholar
  2. Deuser WG, Hunt JM (1969) Stable isotope ratios of dissolved inorganic carbon in the Atlantic. Deep Sea Res 16:221–225Google Scholar
  3. Diaz-Pulido G, McCook LJ (2002) The fate of bleached corals: patterns and dynamics of algal recruitment. Mar Ecol Prog Ser 232:115–128. doi: 10.3354/meps232115 CrossRefGoogle Scholar
  4. Fine M, Loya Y (2002) Endolithic algae: an alternative source of photoassimilates during coral bleaching. Proc R Soc Lond B Biol Sci 269:1205–1210. doi: 10.1098/rspb.2002.1983 CrossRefGoogle Scholar
  5. Giordano M, Beardall J, Raven JA (2005) CO2 concentrating mechanisms in algae: mechanisms, environmental modulation and evolution. Annu Rev Plant Biol 56:99–131. doi: 10.1146/annurev.arplant.56.032604.144052 CrossRefGoogle Scholar
  6. Heikoop JM, Dunn JJ, Risk MJ, Sandeman IM, Schwarcz HP, Waltho N (1998) Relationship between light and the δ15N of coral tissue: examples from Jamaica and Zanzibar. Limnol Oceanogr 43:909–920CrossRefGoogle Scholar
  7. Heikoop JM, Risk MJ, Lazier AV, Edinger EN, Jompa J, Limmon GV, Dunn JJ, Browne DR, Schwarcz HP (2000) Nitrogen–15 signals of anthropogenic nutrient loading in reef corals. Mar Pollut Bull 40:628–636. doi: 10.1016/S0025-326X(00)00006-0 CrossRefGoogle Scholar
  8. Kevekordes K, Holland D, Häubner N, Jenkins S, Kos R, Roberts S et al (2006) Inorganic carbon acquisition by eight species of Caulerpa (Caulerpaceae, Chlorophyta). Phycologia 45:442–449. doi: 10.2216/05-55.1 CrossRefGoogle Scholar
  9. Le Campion-Alsumard T, Golubic S, Hutchings P (1995) Microbial endoliths in skeletons of live and dead corals: Porites lobata (Moorea, French Polynesia). Mar Ecol Prog Ser 117:149–157. doi: 10.3354/meps117149 CrossRefGoogle Scholar
  10. Minagawa M, Wada E (1984) Stepwise enrichment of 15N along food chains: further evidence and the relation between 15N and animal age. Geochim Cosmochim Acta 48:1135–1140. doi: 10.1016/0016-7037(84)90204-7 CrossRefGoogle Scholar
  11. Muscatine L, D’Elia CF (1978) The uptake, retention, and release of ammonium by reef corals. Limnol Oceanogr 23:725–734CrossRefGoogle Scholar
  12. Muscatine L, Kaplan IR (1994) Resource partitioning by reef corals as determined from stable isotope composition II. δ15N of zooxanthellae and animal tissue versus depth. Pac Sci 48:304–312Google Scholar
  13. Muscatine L, Porter JW, Kaplan IR (1989) Resource partitioning by reef corals as determined from stable isotope composition I. δ13C of zooxanthellae and animal tissue versus depth. Mar Biol (Berl) 100:185–193. doi: 10.1007/BF00391957 CrossRefGoogle Scholar
  14. Rau GH, Teyssie JL, Rassoulzadegan F, Fowler SW (1990) 13C/12C and 15N/14N variations among size-fractionated marine particles: implications for their origin and trophic relationships. Mar Ecol Prog Ser 59:33–38. doi: 10.3354/meps059033 CrossRefGoogle Scholar
  15. Raven JA (2003) Inorganic carbon concentrating mechanisms in relation to the biology of algae. Photosynth Res 77:155–171. doi: 10.1023/A:1025877902752 CrossRefGoogle Scholar
  16. Raven JA, Johnston AM, Kübler JE, Korb RE, McInroy SG, Handley LL et al (2002) Mechanistic interpretation of carbon isotope discrimination by marine macroalgae and sea grasses. Funct Plant Biol 29:355–378. doi: 10.1071/PP01201 CrossRefGoogle Scholar
  17. Raven JA, Ball LA, Beardall J, Giordano M, Maberly SC (2005) Algae lacking carbon-concentrating mechanisms. Can J Bot Rev Canadienne Botanique 83:879–890. doi: 10.1139/b05-074 Google Scholar
  18. Risk MJ, Sammarco PW, Schwarcz HP (1994) Cross-continental shelf trends in δ13C in coral on the Great Barrier Reef. Mar Ecol Prog Ser 106:121–130. doi: 10.3354/meps106121 CrossRefGoogle Scholar
  19. Roberts JM, Davies PS, Fixter LM (1999) Symbiotic anemones can grow when starved: nitrogen budget for Anemonia viridis in ammonium-supplemented seawater. Mar Biol (Berl) 133:29–35. doi: 10.1007/s002270050439 CrossRefGoogle Scholar
  20. Rodrigues AU, Grottoli AG (2006) Calcification rate and the stable carbon, oxygen, and nitrogen isotopes in the skeleton, host tissue, and zooxanthellae bleached and recovering Hawaiian corals. Geochim Cosmochim Acta 70:2781–2789. doi: 10.1016/j.gca.2006.02.014 CrossRefGoogle Scholar
  21. Sammarco PW, Risk MJ, Schwarcz HP, Heikoop JM (1999) Cross-continental shelf trends in δ15N in coral on the Great Barrier Reef: further consideration of the reef nutrient paradox. Mar Ecol Prog Ser 180:131–138. doi: 10.3354/meps180131 CrossRefGoogle Scholar
  22. Schlichter D, Zscharnack B, Krisch H (1995) Transfer of photoassimilates from endolithic algae to coral tissue. Naturwissenschaften 82:561–564. doi: 10.1007/BF01140246 CrossRefGoogle Scholar
  23. Swart PK, Saied A, Lamb K (2005) Temporal and spatial variation in the delta N-15 and delta C-13 of coral tissue and zooxanthellae in Montastrea faveolata collected from the Florida reef tract. Limnol Oceanogr 50:1049–1058CrossRefGoogle Scholar
  24. Tanaka Y, Miyajima T, Koike I, Hayashibara T, Ogawa H (2006) Translocation and conservation of organic nitrogen within the coral-zooxanthella symbiotic system of Acropora pulchra, as demonstrated by dual isotope-labeling techniques. J Exp Mar Biol Ecol 336:110–119. doi: 10.1016/j.jembe.2006.04.011 CrossRefGoogle Scholar
  25. Titlyanov EA, Titlyanova TV (2002) Reef-building corals–symbiotic autotrophic organisms: 1. General structure, feeding pattern and light-dependent distribution in the shelf. Russ J Mar Biol 28:1–15. doi: 10.1023/A:1021836204655 CrossRefGoogle Scholar
  26. Titlyanov EA, Novojilov AD, Cherbadgy II (1993) Ahnfeltia tobuchiensis: Biology, ecology, productivity. “Nauka” Moscow, 208 pGoogle Scholar
  27. Titlyanov EA, Titlyanova TV, Yakovleva IM, Nakano Y, Bhagooli R (2005) Regeneration of artificial injuries on scleractinian corals and coral/algal competition for newly formed substrate. J Exp Mar Biol Ecol 323:27–42. doi: 10.1016/j.jembe.2005.02.015 CrossRefGoogle Scholar
  28. Titlyanov EA, Kiyashko SI, Titlyanova TV, Yakovleva IM, Wada E (2006) Coral-algal competition as determined from the rate of overgrowth, physiological condition of polyps of the scleractinian coral Porites lutea, and structure of algal associations within boundary areas. Proc 10th int coral reef symp, pp 1931–1942Google Scholar
  29. Titlyanov EA, Titlyanova TV, Yakovleva IM, Sergeeva OS (2007) Interaction between benthic algae (Lyngbya bouillonii, Dictyota dichotoma) and scleractinian coral Porites lutea in direct contact. J Exp Mar Biol Ecol 342:282–291CrossRefGoogle Scholar
  30. Tribollet A, Landon C, Golubic S, Atkinson M (2006) Endolithic microflora are major primary production in dead carbonate substrates of Hawaiian Coral Reefs. J Phycol 42:290–303. doi: 10.1111/j.1529-8817.2006.00198.x CrossRefGoogle Scholar
  31. Wang WL, Yeh HW (2003) Delta C-13 values of marine macroalgae from Taiwan. Bot Bull Acad Sin 44:107–112Google Scholar
  32. Williams PM, Gordon LI (1970) Carbon-13: carbon-12 ratios in dissolved and particulate organic matter in the sea. Deep-Sea Res 17:19–27Google Scholar
  33. Yamamuro M, Kyanne H, Minagawa M (1995) Carbon and nitrogen stable isotopes of primary producers in coral reef ecosystems. Limnol Oceanogr 40:617–621CrossRefGoogle Scholar
  34. Yellowlees D, Rees TA, Fitt WK (1994) Effect of ammonium-supplemented seawater on glutamine synthetase and glutamate dehydrogenase activities in host tissue and zooxanthellae of Pocillopora damicornis and on ammonium uptake rates of the zooxanthellae. Pac Sci 48:291–295Google Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Eduard A. Titlyanov
    • 1
    • 2
  • Serguei I. Kiyashko
    • 1
  • Tamara V. Titlyanova
    • 1
  • Tatyana L. Kalita
    • 1
  • John A. Raven
    • 3
  1. 1.Institute of Marine BiologyFar East Branch of Russian Academy of SciencesVladivostokRussia
  2. 2.Tropical Biosphere Research CenterUniversity of the RyukyusOkinawaJapan
  3. 3.Division of Plant SciencesUniversity of Dundee at SCRI, Scottish Crop Research InstituteDundeeUK

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